Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides

Han, Zi-Hua; Ye, Jianmin; Wang, Guanfu

doi:10.1016/j.ijbiomac.2012.09.009

Cited by 93 publications

(41 citation statements)

References 24 publications

(24 reference statements)

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“…Hericium erinaceus polysaccharide is a traditional Chinese medicine [12], and beneficial nutrient native to China and other Asian countries, which has previously been employed for treating cancers, lymphatic disease and gastroenteric disorders [13]. Although the involvement of erinacine in HCC has been identified, there's very little known on the molecular mechanisms by which erinacine suppresses human HCC cell growth.…”

Section: Introductionmentioning

confidence: 99%

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Erinacine, which is extracted from the medicinal mushroom Hericium erinaceus, is known to play anticancer roles in human cancers. The following study aims to investigate the role of erinacine in the opening of the mitochondrial permeability transition pore (MPTP) in hepatocellular carcinoma (HCC) through the PI3K/Akt/GSK-3β signaling pathway and highlights the applicability of erinacine in HCC treatments. Methods: HCC and paracancerous tissues were obtained from 85 HCC patients who’ve undergone surgical resection. Immunohistochemistry was adopted to detect positive expression of PI3K, Akt, and GSK-3β. Treatment of HepG-2 with LY294002 (an inhibitor of the PI3K/Akt/GSK-3β signaling pathway) and different concentration of erinacine was performed to determine the involvement of LY294002 in erinacine action. The expressions of PI3K, Akt, GSK-3β, CyclinD1, Vimentin, β-catenin, Bcl-2, E-cadherin, Bax, and caspase-9 were determined by RT-qPCR and Western blot analysis. Cell viability, colony formation rate, migration, invasion, cycle, and apoptosis were detected by MTT, colony formation, wound healing assay, Transwell assay, and flow cytometry, respectively. The size and weight of xenograft tumors were observed in nude mice. Mitochondrial membrane potential in HepG-2 was determined using laser scanning confocal microscopy following JC-1 staining. Mitochondrial Ca²⁺ indicator Rhod-2, AM was used to detect the changes of mitochondrial Ca²⁺, while western blot analysis was employed to detect the presence levels of cytochrome C (cyt-C). Results: The results revealed that PI3K, Akt, and GSK-3β were up-regulated in HCC tissues. Erinacine or LY294002 led to a decrease in mitochondrial membrane potential, increase in intracellular mitochondrial Ca²⁺, and the release of cyt-C in mitochondria. In addition, Erinacine was found to decrease the mitochondrial membrane potential, expression of PI3K, Akt, GSK-3β, CyclinD1, Vimentin, β-catenin, and Bcl-2, cell proliferation, colony formation ability, migration, invasion, and xenograft tumor size, while E-cadherin, Bax, and caspase-9 expression, and cell apoptosis were elevated in a dose-dependent manner. Erinacine also stimulated the effects of LY294002 on the HCC. Following the addition of 500 μM Erinacine and MPTP opening inhibitor CsA, we found that the mitochondrial membrane potential level increased, while mitochondrial Ca2+ and Cyt-C decreased from the mitochondria. Conclusion: The results from the study demonstrated that erinacine induced MPTP opening, facilitates the release of cyt-C, and inhibited cell proliferation, migration, and invasion, while it promotes apoptosis by inactivating the PI3K/Akt/GSK-3β signaling pathway, preventing the progression of HCC.

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Section: Introductionmentioning

confidence: 99%

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Zhou

et al. 2018

Cell Physiol Biochem

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“…Kim SP, et al demonstrated that the extracts from Hericium erinaceus against bacterium infection in mice occur through the activation of innate immune cells [12]. Han ZH, et al reported that Hericium erinaceus can significantly decrease lipid peroxidation level and increase antioxidant enzymes activities in experimental animals [15]. Many studies have been performed for examining its pharmacological function in many diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Hericium erinaceus has been reported to significantly decrease lipid peroxidation level and increase antioxidant enzymes activities in experimental animals [15]. Yang BK, et al reported that an exo-biopolymer produced from a submerged mycelial culture of Hericium erinaceus possesses hypolipidemic effect in dietary-induced hyperlipidemic rats [16], but the effects of Hericium erinaceus on hyperglycaemia, hyperlipidemia, lipid peroxidation and antioxidant enzymes activities in diabetes have not yet been examined.…”

Section: Introductionmentioning

confidence: 99%

Antihyperglycemic and antihyperlipidemic activities of aqueous extract of Hericium erinaceus in experimental diabetic rats

Liang

Guo

Xie

et al. 2013

BMC Complement Altern Med

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BackgroundHericium erinaceus, as a commonly used medicine or food, has attracted much attention due to its health effects when used as a home remedy for some diseases. The aim of this work was to investigate the hypoglycemic and hypolipidemic effects of aqueous extract of Hericium erinaceus (AEHE) in streptozotocin (STZ)-induced diabetic rats.MethodsDiabetes was induced in Wistar rats by the administration of STZ (55 mg/kg BW.) intraperitoneally. AEHE (100 and 200 mg/kg BW.) was administered for a period of 28 days. The effects of AEHE on glucose, insulin, and lipid files in blood, and oxidative stress parameters in the liver were evaluated. The body weights of rats were recorded at day 0, 14 and 28th days.ResultsThe administration of AEHE for 28 days in STZ diabetic rats resulted in a significant decrease in serum glucose level and a significant rise in serum insulin level. AEHE treatment attenuated lipid disorders. In addition, AEHE administration increased the activities of CAT, SOD, and GSH-Px, and GSH level, and reduced MDA level in the liver tissue significantly.ConclusionOur results suggest that AEHE possesses hypoglycemic, hypolipidemic, and antioxidant properties in STZ-induced diabetes rats.

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“…In literature, arabinose has been reported as one of the minor sugars present in mushrooms (Kalac, 2012), but for the species studied herein, arabinose was the major sugar. High levels of arabinose seem to be a characteristic of the genus Hericinum (Keong, Rashid, Ing & Ismail, 2007;Han, Ye & Wang, 2013). Regarding fatty acids composition of H. erinaceus, saturated fatty acids (SFA) predominated over polyunsaturated (PUFA) and monounsaturated (MUFA) fatty acids, due to the high contents of palmitic (C16:0) and stearic (C18:0) acids (Table 1).…”

Section: Chemical Compositionmentioning

confidence: 99%

Chemical composition, antioxidant activity and bioaccessibility studies in phenolic extracts of two Hericium wild edible species

Heleno

Barros

Martins

et al. 2015

LWT - Food Science and Technology

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Mushrooms are rich sources of bioactive compounds such as phenolic acids. When ingested, these molecules have to be released from the matrix to be transformed/absorbed by the organism, so that they can exert their bioactivity. Several in vitro methodologies have been developed in order to evaluate the bioavailability of bioactive compounds. Herein, two Hericium species were analyzed for their chemical composition and antioxidant activity. Furthermore, an in vitro digestion of the mushrooms and mushroom phenolic extracts was performed, and the digested samples were also submitted to antioxidant activity evaluation in order to evaluate the bioaccessibility of the phenolic acids identified in the samples. Hericium species showed similar chemical profiles (except for tocopherols), varying only in the concentration of the compounds. The phenolic extracts revealed higher antioxidant activity than the in vitro digested samples, meaning that this process decrease the antioxidant properties of the extract/mushroom. Nevertheless, phenolic acids were found in the digested samples, meaning that those molecules are bioaccessible.

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Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides

Cited by 93 publications

References 24 publications

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3β Signaling Pathway in Human Hepatocellular Carcinoma

Antihyperglycemic and antihyperlipidemic activities of aqueous extract of Hericium erinaceus in experimental diabetic rats

Chemical composition, antioxidant activity and bioaccessibility studies in phenolic extracts of two Hericium wild edible species

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