Evaluation of in vitro Probiotic Potential of Pediococcus pentosaceus OZF Isolated from Human Breast Milk

Abstract

This study was conducted to evaluate the probiotic properties of Pediococcus pentosaceus OZF isolated from human breast milk. The results obtained so far suggest that the strain is resistant to low pH, bile salt, pepsin and pancreatin, so it could survive while passing through the upper part of the gastrointestinal tract and reveal its potential probiotic action on host organism. The strain was non-pathogenic (γ-hemolytic), produced anti-Listerial bacteriocin, exhibited a strong autoaggregating phenotype (85.71%) and demonstrated 6.26 and 12.99% coaggregation with Salmonella enterica serotype Typhimurium SL 1344 and Escherichia coli LMG 3083 (ETEC), respectively. The degree of adhesion of Ped. pentosaceus OZF to the human Caco-2 cell line was investigated and when compared to the adhesion of pathogenic strains tested, it was shown to inhibit the growth of human enterotoxigenic E. coli LMG 3083 (ETEC) and of Salm. Typhimurium SL 1344. Ped. pentosaceus OZF seems to adhere to human intestinal cells via mechanisms that involve different combinations of carbohydrate and lipid factors on the bacteria and eukaryotic cell surface. The percentage of adhesion to n-hexadecane was 34% showing that the surface was rather hydrophilic. Higher affinity displayed by Ped. pentosaceus OZF for chloroform demonstrates the basic property of a cell, which may be due to the presence of carboxylic groups on the cell surface.

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

“…For the pathogen (Listeria monocytogenes MTCC 657) exclusion assay, the monolayers were first treated with the LAB isolates (5 × 10 10 -10 11 CFU mL −1 ) for 90 min, washed with PBS to remove non-adhered LAB followed by addition of Listeria cells (2 × 10 10 CFU mL −1 ) suspended in serum and antibiotic free medium and incubated for 60 min [20]. The excluded Listeria cells were recovered by washing with the PBS and were plated on Listeria selective agar [28]. The amount of excluded Listeria cells were calculated as the amount of Listeria cells recovered after incubation compared to initial amount added to the cell lines with the following formula, Amount of excluded Listeria cells = log (X) − log (Y) where X is the no.…”

Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

“…Pediococcus pentosaceus and Lactobacillus salivarius are most frequently isolated from the duodenal mucosa of piglets (Zhang et al, 2011;Zhang et al, 2015) and human breast milk (Osmanagaoglu et al, 2010;Kiran et al, 2015), but little information is available about the isolated strains from animals and their bioavailability. Therefore, the objectives of this study were to isolate and identify P. pentosaceus and L. salivarius strains from cecal content and investigate their potential probiotic effects on growth, meat quality and microenvironment in specific pathogen free (SPF) chickens, and ammonia treatment in vitro.…”

Molecular Characterization of Bacteriocinogenic, Antifungal and Probiotic Lactic Acid Bacteria Isolated from Chicken Gastrointestinal Tract

“…The aqueous phase was collected and absorbance was checked at 600 nm. The optical density readings were used to calculate the percentage of cell surface hydrophobicity of isolate adhering to solvent [23].…”