Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Abstract

Objectives: This study investigates the in vitro anticancer activity of the violacein extracted from the Chromobacterium vaccinii CV5.Methods: Natural colorants or dyes derived from flora to fauna are believed to be safe because of nontoxic, noncarcinogenic, and biodegradable in nature. There are a number of natural pigments, but only a few are available in sufficient quantities for industrial production. The cytotoxicity activity of pigment was assessed against the cervical (HeLa) and lung cancer (A549) cell lines using the MTT assay and there by potential cytotoxic activity exhibited by the pigment was identified. Results:The result of the pigment shows potent anticancer activity on the two cancer cell lines tested in a concentration dependent manner. The potent anticancer activity was observed with the pigment with IC 50 values of 26 µg/mL on HeLa and 31 µg/mL on A549 cells, respectively. Conclusion:The study is pioneering report for determining the better in vitro anticancer activity of violacein from the novel isolate C. vaccinii CV5.

Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells.

“…The divergences in its IC50 value from other studies suggested the presence of various cell-typespecific mechanisms that operate in the existence of violacein. (Alshatwi et al, 2016, Santhosh andMuthusamy, 2017). The violacein antiproliferative effect achieved by reactive oxygen species (ROS) production in cancer, causing apoptosis promoting via enhances of intracellular calcium and release of cytochrome c (De Carvalho et al, 2006).…”