Evaluation of in vitro Antioxidant Activities of Ethanol Extracts of Datura innoxia Mill. Leaves and Seeds Harvested in Mali

Abstract

Aims: The purpose of this study was to evaluate the in vitro antioxidant activities of Datura innoxia Mill. leaves and seeds harvested in Mali.
 Place and Duration of Study: Collection of plant materials were done at Kolondieba (Mali) in june 2016. Evaluation of polyphenols and flavonoids contents, determination of antioxidant activities were done at Laboratory of Plant and Food Biochemistry and Biotechnology of University of Sciences, Techniques and Technologies of Bamako (Mali) between august 2016 and March 2017.
 Methodology: The leaves and seeds were collected in Kolondieba, Mali. The phytochemical screening based on the standard methods of tube reactions has been performed with ethanol extracts. The quantitative estimation of total polyphenols was made by the Folin-Ciocalteu method and that of flavonoids by the use of aluminum trichloride. The in vitro antioxidant activities of the ethanol extracts was determined by three methods: the 1,1- diphenyl - 2- picrylhydrazyl (DPPH) test, the ferric reducing ability power (FRAP) test and the Phosphomolybdate test (PPM).
 Results: The phytochemical screening revealed that leaves and seeds of this plant contains alkaloids, polyphenols, flavonoids, coumarins, tannins, triterpenes and saponin. Phenolic contents of ethanol extracts are 30.97 ± 0.33 mg equivalent gallic acid / g in leaves and 14.02 ± 0.15 mg equivalent gallic acid / g in seeds; those of flavonoids are 15.13 ± 0.2 mg equivalent of quercetin / g in the leaves and 4.93 ± 0.41 mg equivalent of quercetin / g in the seeds. The three tests showed that the leaves have a higher level of antiradical activity in vitro than seeds.
 Conclusion: The results of this work showed that Datura innoxia Mill. has a good antioxidant activity which would justify its use as a potential source of natural antioxidants.

Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali

“…The ANOVA using the Dunnett Test showed that the antiradical activities of our bark extracts (p-value = 0.004 < 0.05) and leaves (p-value = 0.0001 < 0.05) are all lower than those of the positive controls tested: ascorbic acid with 3.05 ± 0.07 µg/ml, rutin with 2.79 ± 0.79 µg/ml and quercetin with 1.17 ± 0.03 µg/ml (Figure 4.). The high content of polyphenols and flavonoids (Figure 2) in the extracts would be responsible for the anti-free radical activities observed in our extracts; since a direct correlation between antioxidant activity and the reducing power of plant extracts involving phenolic compounds has been reported by many authors (Fatima et al, 2015;Iqbal et al, 2017;Togola et al, 2019;Fofié et al, 2017;Bagewadi et al, 2019). Other secondary metabolites (tannins, terpenes, sterols, etc.…”

Evaluation of the Antioxidant Activity of Some Plants Used in the Management of Erectile Dysfunction by the BWA Community in Mali

“…Polyphenols were determined by spectrophotometric method using the Folin-Ciocalteu reagent according to the protocol of 15 , slightly modified by 16 . Briefly, 500 µL of Folin-Ciocalteu reagent (diluted 10% in distilled water) was added to 100 µL of extract with well determined concentrations.…”

“…The Figure 2 summarises the antioxidant activity of the extracts of the three plants studied and this activity is expressed in IC50. Analysis of these data showed that the antioxidant power varies according to the solvent (p=0.4.10- 16 ) and and the species (p=0.35.10 -15 ).…”

Phytochemistry and in Vitro Antioxidant Activities of Four Consumed Picking Products in Sikasso, Mali

“…The trapping activity of the radical DPPH was measured according to the protocol described by Togola et al (2019a). From a solution of 100 µg/mL extract, a calibration range was established.…”

“…Studies have shown that many edible wild plants have a high level of antioxidant activity (Adjdir et al, 2018). Several metabolites are involved in the antioxidant and antimicrobial activities of plant extracts (Fatima et al, 2015;Bagewadi et al, 2019;Togola et al, 2019a). Among these metabolites, total polyphenol and flavonoid are predominant.…”