Evaluation of in vitro aldose reductase inhibitory potential of different fraction of Hybanthus enneaspermus Linn F. Muell

daptation of the in vitro Culture of Origanum majorana L. For Production of Phenolic Acids

“…BAP has a marked effect on stimulating the growth of auxiliary and foliar development of shoot tip cultures [35]. Most Hybanthus enneapermus culture systems were achieved by using BAP as a supplement to basal media [36].…”

Exploration of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays

“…After phytochemical investigations of the leaf extracts of EG, EC and ECA, in vitro-inhibitory assays of α-amylase, α-glucosidase, aldose reductase, ACE, DPP4, antioxidant assays like DPPH free radical scavenging activity, scavenging of hydrogen peroxide and total antioxidant activity in the ferric reducing antioxidant power (FRAP) assay were carried out following standard methods [46][47][48][49][50][51][52][53][54][55][56][57] . The enzymes mentioned above contribute to the pathogenesis of type 2 DM in one way or another.…”

“…Briefly, 2 mg of starch azure Aldose reductase inhibitory assay: The assay was carried out following reported literature methods and the experimental protocol approved by the Institutional Ethical Committee [48][49][50] . Two to three mo old healthy adult Wistar albino rats weighing about 150-200 g were acclimatized to laboratory conditions (12 h light and 12 h dark cycle, 25 ± 5 ℃, 30%-60% relative humidity) with free access to pelleted food and water ad libitum.…”

“…Next, a 10% homogenate was prepared from the rat lens in 0.1 mol/L phosphate-buffered saline (PBS) at pH 7.4, centrifuged at 5000 g for 10 min in the cold and the supernatant collected. The protein content of the supernatant was determined by literature methods [48][49][50] . For the determination of the aldose reductase (AR) inhibitory activity, 0.7 mL of phosphate buffer (0.067 mol), 0.1 mL of NADPH (25 × 10 -5 mol), 0.1 mL of DL-glyceraldehyde (substrate, 5 × 10 -4 mol) and 0.1 mL of lens supernatant were mixed in the sample cuvette.…”

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

“…Several authors have studied and reported on a number of structurally diverse naturally occurring and synthetic AR inhibitors that have proven to be effective for the prevention of diabetic complications in experimental animals, as well as in clinical trials. 14,15 Although, some synthetic aldose reductase inhibitors (ARIs) have been developed as drug candidates, however, virtually all have not been successful in clinical trials due to adverse pharmacokinetic properties, inadequate efficacy, and toxic side effects. The use of medicinal plants, plant extracts or plant-derived pure chemicals to treat human ailments is an important alternative therapeutic approach.…”