Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections

Vázquez, Susana; Hafner, Greg; Ruiz, Didye; Calzada, Naifi; Guzmán, María G.

doi:10.1016/j.jcv.2007.04.003

Cited by 52 publications

(41 citation statements)

References 37 publications

(37 reference statements)

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“…However, this is likely a minor effect because the prevalence of IgM antibodies against dengue was similar among patients who came to a hospital within the first four days of symptom onset (9.4%) compared with patients who came to a hospital within 5-10 days of symptom onset (9.7%). Moreover, the sensitivity of the assay was high, ranging from 83.5% to 96.8%, as reported in several studies, [55][56][57][58] irrespective of the sample collection period from the onset of illness.…”

Section: 54mentioning

confidence: 66%

“…However, the high specificity (99%) of the PanBio (Windsor, Queensland, Australia) IgM ELISA reported in several studies suggests that few of our cases were misclassified. 56,57,60 In Bangladesh, dengue is a year-round threat in urban and rural areas. As vaccine trials continue, 61, 62 a community-based dengue vaccination campaign could be piloted and evaluated as a possible prevention strategy.…”

Section: 54mentioning

confidence: 99%

See 1 more Smart Citation

Hospital-Based Prevalence of Malaria and Dengue in Febrile Patients in Bangladesh

Faruque

Zaman

Alamgir³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Acknowledgments: We thank the head of the departments of medicine and pediatric units of the participating hospitals for their consistent support; the surveillance physicians and field assistants for their continuous effort in collecting data; Abdur Rouf Siddique and Md. Zakaria Hossain for providing technical support for sample collection throughout the study; Dorothy Southern for her guidance in writing and reviewing the drafts; and M. Abu Yushuf Sharker for his statistical support in data analysis.

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Section: 54mentioning

confidence: 66%

Section: 54mentioning

confidence: 99%

Hospital-Based Prevalence of Malaria and Dengue in Febrile Patients in Bangladesh

Faruque

Zaman

Alamgir³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…Although a positive anti-DENV IgM result in the setting of an acute febrile illness is often considered diagnostic of acute DENV infection, this approach can be problematic because anti-DENV IgM is often detectable for 2-3 months after initial infection. 41 Moreover, the reported sensitivity and specificity of the DENV serologic assays used in this study have varied widely against conventional standard tests [42][43][44][45] and little is known about the performance of any DENV serologic assay in East Africa. Consequently, positive anti-DENV serology has obvious shortcomings for confirming acute symptomatic DENV infection in our study.…”

Section: Discussionmentioning

confidence: 99%

Chikungunya and Dengue Fever among Hospitalized Febrile Patients in Northern Tanzania

Hertz

Munishi²,

Ooi³

et al. 2012

The American Society of Tropical Medicine and Hygiene

121

129

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Abstract. Consecutive febrile admissions were enrolled at two hospitals in Moshi, Tanzania. Confirmed acute Chikungunya virus (CHIKV), Dengue virus (DENV), and flavivirus infection were defined as a positive polymerase chain reaction (PCR) result. Presumptive acute DENV infection was defined as a positive anti-DENV immunoglobulin M (IgM) enzyme-linked immunsorbent assay (ELISA) result, and prior flavivirus exposure was defined as a positive anti-DENV IgG ELISA result. Among 870 participants, PCR testing was performed on 700 (80.5%). Of these, 55 (7.9%) had confirmed acute CHIKV infection, whereas no participants had confirmed acute DENV or flavivirus infection. Anti-DENV IgM serologic testing was performed for 747 (85.9%) participants, and of these 71 (9.5%) had presumptive acute DENV infection. Anti-DENV IgG serologic testing was performed for 751 (86.3%) participants, and of these 80 (10.7%) had prior flavivirus exposure. CHIKV infection was more common among infants and children than adults and adolescents (odds ratio [OR] 1.9, P = 0.026) and among HIV-infected patients with severe immunosuppression (OR 10.5, P = 0.007). CHIKV infection is an important but unrecognized cause of febrile illness in northern Tanzania. DENV or other closely related flaviviruses are likely also circulating.

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“…Because all serum samples were collected early after fever onset (between 0 and 5 days), a sample was defined as primary infection when negative for IgG and as secondary infection when positive for IgG. 24,25 The presence of NS1 was qualitatively determined using the Platelia™ NS1 Ag enzyme immunoassay (Bio-Rad Laboratories, Marnes-la-Coquette, France) as indicated by the manufacturer. For quantification of NS1 levels in serum samples with the same assay, standard curves were constructed by using two-fold serial dilutions of affinity-purified recombinant NS1 from DENV-2 expressed in Escherichia coli (kindly provided by Dr. Ronaldo Mohana-Borges, Univesidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil).…”

Section: Introductionmentioning

confidence: 99%

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Cruz-Hernández¹,

Flores-Aguilar²,

González-Mateos³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity.

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Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections

Cited by 52 publications

References 37 publications

Hospital-Based Prevalence of Malaria and Dengue in Febrile Patients in Bangladesh

Hospital-Based Prevalence of Malaria and Dengue in Febrile Patients in Bangladesh

Chikungunya and Dengue Fever among Hospitalized Febrile Patients in Northern Tanzania

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

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