Evaluation of<i>Ochrobactrum anthropi</i>TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease

The rhizosphere is the soil-plant root interphase and in practice consists of the soil adhering to the root besides the loose soil surrounding it. Plant growth-promoting rhizobacteria (PGPR) are potential agents for the biological control of plant pathogens. A biocontrol strain should be able to protect the host plant from pathogens and fulfill the requirement for strong colonization. Numerous compounds that are toxic to pathogens, such as HCN, phenazines, pyrrolnitrin, and pyoluteorin as well as, other enzymes, antibiotics, metabolites and phytohormones are the means by which PGPR act, just as quorum sensing and chemotaxis which are vital for rhizosphere competence and colonization. The presence of root exudates has a pronounced effect on the rhizosphere where they serve as an energy source, promoting growth and influencing the root system for the rhizobacteria. In certain instances they have products that inhibit the growth of soil-borne pathogens to the advantage of the plant root. A major source of concern is reproducibility in the field due to the complex interaction between the plant (plant species), microbe and the environment (soil fertility and moisture, day length, light intensity, length of growing season, and temperature). This review listed most of the documented PGPR genera and discussed their exploitation.

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“…Ochrobactrum anthropi TRS-2 (Chakraborty et al 2009) isolated from the rhizosphere of tea produces siderophore and IAA in vitro. Pseudomonas spp.…”

Section: Production Of Siderophoresmentioning

confidence: 99%

Beneficial bacteria of agricultural importance

2010

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“…In our previous studies, Ochrobactrum anthropi BMO-111 was more efficient than chemical fungicides in controlling blister blight diseae of tea (Sowndhararajan et al 2013). In support to our study, the biological control potential of the bacterium O. anthropi in controlling the fungal diseases of plants under field and in vitro conditions were evaluated by some authors (Chaiharn et al 2009;Chakraborty et al 2009). Therefore, an attempt was made to reducing the use of chemicals by combined and alternate with O. anthropi BMO-111 in integrated management practices against blister blight disease of tea.…”

Section: Introductionmentioning

confidence: 65%

Integrated control of blister blight disease in tea using the biocontrol agentOchrobactrum anthropistrain BMO-111 with chemical fungicides

Sowndhararajan

Marimuthu²,

Manian

2013

J Appl Microbiol

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Aims: The present study was aimed to evaluate the integration of Ochrobactrum anthropi BMO-111 and chemical fungicides (copper oxychloride and hexaconazole) against blister blight disease of tea. Methods and Results: Application of the liquid culture of O. anthropi BMO-111 (36-h-old culture broth) was found to be effective in combined sprays with individual chemical fungicides (copper oxychloride and hexaconazole). Spray application of O. anthropi BMO-111 to tea bushes improved the biochemical parameters such as the levels of chlorophyll, polyphenols, and catechins in the harvestable tea shoots. Moreover, in the microplot and large scale trials, the integrated treatment of every two O. anthropi BMO-111 sprays followed by a single fungicides spray was found to be more efficient than the stand alone O. anthropi BMO-111 or chemicals sprays. Further, pathogenicity study employing Swiss albino mice showed no mortality in the test animals when challenged with O. anthropi BMO-111 through oral, intravenous and intranasal routes. Conclusions: The field trials clearly established that O. anthropi BMO-111 has capability to reduce incidence in integrated management of blister blight disease of tea and safe to use in the field. Significance and Impact of the Study: The results indicate that O. anthropi BMO-111 can be used as an agricultural input in the integrated crop protection systems.

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“…The efficacy of antagonistic organisms that protect plants from root rot disease has been evaluated. For instance, Chakraborty et al (2009) reported that Ochrobactrum anthropi TRS-2 isolated from tea rhizospheres solubilized phosphates, produced siderophores and indole-3-acetic acid in vitro, and exhibited antifungal activity against six test pathogens. O. anthropi treatment also decreases the incidence of brown root rot caused by P. noxius in tea (Chakraborty et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, Chakraborty et al (2009) reported that Ochrobactrum anthropi TRS-2 isolated from tea rhizospheres solubilized phosphates, produced siderophores and indole-3-acetic acid in vitro, and exhibited antifungal activity against six test pathogens. O. anthropi treatment also decreases the incidence of brown root rot caused by P. noxius in tea (Chakraborty et al, 2009). With regard to rubber root disease, Trichoderma isolates obtained from rubber-growing areas have antagonistic effects on Rigidoporus microporus, and Trichoderma harzianum can also control R. microporus in vitro (Jayasuriya and Thennakoon, 2007 (Ogbebor et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Identification of lipopeptides produced by Bacillus subtilis Czk1 isolated from the aerial roots of rubber trees

Fan

et al. 2017

Genet. Mol. Res.

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ABSTRACT. We obtained a strain of Bacillus subtilis, which we named Czk1, from the aerial roots of rubber trees. This bacterial isolate exhibits strong antagonistic activity against Ganoderma pseudoferreum, Phellinus noxius, Helicobasidium compactum, Rigidoporus lignosus, Sphaerostilbe repens, and Colletotrichum gloeosporioides. Our earlier research has shown that the antagonistic activity of a fermentation supernatant Czk1 isolate produces a complex mixture of lipopeptides. In this study, we used methanol to extract crude lipopeptides, purified them using a Sephadex G-25 column, cloned the lipopeptide genes, and analyzed purified fractions by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF-MS) to identify the lipopeptides from B. subtilis strain Czk1. The cloned lipopeptide genes included those that encode the enzymes lpa, ituD, sfp, and fenB. The crude lipopeptides were purified and found in five fractions. Further analysis revealed that five fractions of the purified composition contained members of the surfactin, iturin, fengycin, and bacillomycin families of antibiotics. This suggests that these lipopeptides from strain Czk1 have potential as plant disease biocontrol agents.

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Evaluation ofOchrobactrum anthropiTRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease

Cited by 92 publications

References 34 publications

Beneficial bacteria of agricultural importance

Beneficial bacteria of agricultural importance

Integrated control of blister blight disease in tea using the biocontrol agentOchrobactrum anthropistrain BMO-111 with chemical fungicides

Identification of lipopeptides produced by Bacillus subtilis Czk1 isolated from the aerial roots of rubber trees

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