Evaluation of Hydroxyl Radical-Scavenging Ability by Chemiluminescence

Hirayama, Osamu; Yida, Masakatsu

doi:10.1006/abio.1997.2282

Cited by 20 publications

(15 citation statements)

References 8 publications

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“…19 where AUC control and AUC sample represent the area under the curve (AUC) of luminol chemiluminescence kinetics in the control and that in samples, respectively.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Soy Isoflavone Genistein on Ischemia-Reperfusion in the Rat Small Intestine

Sato

Itagaki

Oikawa

et al. 2011

Biological & Pharmaceutical Bulletin

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Ischemia-reperfusion (I/R) injury of the intestine is an important factor associated with high rates of morbidity and mortality. Intestinal I/R is a common clinical problem in the settings of severe burns, circulatory shock and strangulation ileus. Intestinal I/R damages remote organs and promotes multi-organ failure. It has been shown that enteral feeding before ischemic insults is beneficial for reducing organ injury and improving survival after intestinal I/R. In that study, the authors used a standard complex enteral diet and they suggested that it is important to find new nutrient formulas. Since reactive oxygen species are responsible for intestinal I/R injury, we focused on a dietary polyphenol, the soy isoflavone genistein. Genistein has a wide spectrum of biochemical and pharmacological activities. However, the possibility of a protective effect of genistein as enteral nutrition on I/R injury has not been investigated. We therefore investigated the protective effect of genistein on oxidative injury using intestinal I/R model rats. We found that genistein, which has combined antioxidant activity from radical scavenging, xanthine oxidase inhibition and chain-breaking effects, exhibits a protective effect on intestinal I/R injury. The results suggest that genistein, a soy isoflavone, has the possibility as a new nutrient formula of enteral feeding.

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“…19 where AUC control and AUC sample represent the area under the curve (AUC) of luminol chemiluminescence kinetics in the control and that in samples, respectively.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Soy Isoflavone Genistein on Ischemia-Reperfusion in the Rat Small Intestine

Sato

Itagaki

Oikawa

et al. 2011

Biological & Pharmaceutical Bulletin

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“…The CL-based determination of scavenging capacity against HO • using luminol has also been described [117][118][119][120]. In these cases, HO • is generated from the reaction between ferrous iron with molecular oxygen, which induces luminol CL.…”

Section: Hydroxyl Radical (Ho • ) Scavenging Capacity Assaysmentioning

confidence: 99%

Methodological aspects about in vitro evaluation of antioxidant properties

Magalhães

Segundo

Reis

et al. 2008

Analytica Chimica Acta

618

404

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“…The method is based on the generation of HO Å by Fenton reaction and the determination of HO Å by chemiluminescence (Hirayama & Yida, 1997). For the Fenton reaction, H 2 O 2 solution (1 mM) was prepared by diluting hydrogen peroxide solution with 25 mM imidazole-nitrite solution (pH 7.0); ferrous sulphate solution (1 mM) was prepared by dissolving FeSO 4 Á 7H 2 O in distilled water and DTPA solution (1 mM) was prepared in distilled water.…”

Section: Measurement Of Hydroxyl Radical-scavenging Activitymentioning

confidence: 99%