Evaluation of hydrophilic interaction chromatography (HILIC) versus C18 reversed-phase chromatography for targeted quantification of peptides by mass spectrometry

Evaluation of the Effects of Sample Dilution and Volume in Hydrophilic Interaction Liquid Chromatography

“…A large number of HILIC applications have been reported recently, most of which include the determination of highly polar compounds such amino acids [8], organic acids [9], and polar drugs [10]. However, there are some drawbacks compared with RPLC, such as its longer equilibration time and lower robustness [6,11]. Sample preparation is another issue that influences the analyte peak shape and separation efficiency significantly.…”

“…In contrast to reversed-phase liquid chromatography (RPLC), HILIC has several advantageous features such as its increased retention of polar compounds with complete avoidance of ion-pair reagents [2,3], its good peak shape for basic compounds [4], its high sensitivity in mass spectrometry because of the highly organic mobile phase [5,6], and the possibility of applying higher flow rates, which can shorten the analysis time significantly [7]. A large number of HILIC applications have been reported recently, most of which include the determination of highly polar compounds such amino acids [8], organic acids [9], and polar drugs [10].…”

Performance of zwitterionic hydrophilic interaction LC for the determination of iodinated X‐ray contrast agents

“…To circumvent this high ion‐suppression effect, the eluted SPE extract was diluted to 5 mL instead of 1 mL and, with this strategy, the ion suppression decreased to values up to 50% at most. This high matrix effect has already been reported when HILIC was used, since this type of SP may become overloaded more easily and the sensitivity may be seriously compromised in real samples . Therefore, the signal improvement achieved in ultrapure water using HILIC was lost in complex samples.…”

Absolute quantification of dengue virus serotype 4 chimera vaccine candidate in Vero cell culture by targeted mass spectrometry

“…Beside selectivity, robustness should also be given priority consideration when hundreds of assays are carried out over a long period. If sufficient amount of sample is available, recent studies attempt to show that conventional flow is a more judicious choice than nanoflow to meet this expectation . Moreover, the accuracy, selectivity, and multiplexing capability of SRM were recently demonstrated as suitable to quantify viral proteins of Avian H7 Subtype of Influenza virus in order to streamline and speed‐up vaccine production .…”