Evaluation of homologous and heterologous protection induced by a virulent field strain of orf virus and an orf vaccine in goats

Musser, Jeffrey M.B.; Waldron, D. F.; Taylor, Charles A.

doi:10.2460/ajvr.73.1.86

Cited by 10 publications

(17 citation statements)

References 25 publications

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“…Their daily body temperature was recorded. Kids were observed during the quarantine for clinical signs of CE and other diseases (Buddle and Pulford, 1984; Pye, 1990;Musser et al 2012).…”

Section: Vaccination Of Rabbits Lambs and Kids With Ce Vaccinementioning

confidence: 99%

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Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Gülyaz¹,

Saraç

Hasöksüz³

et al. 2020

Etlik Veteriner Mikrobiyoloji Dergisi

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Contagious ecthyma (CE) is a zoonotic viral infection and common localized cutaneous infection of young sheep and goats caused by a Parapoxvirus with worldwide distribution. The aim of this study was to determine whether rabbits will be used instead of lambs and kids in immune and safety studies after the production of CE vaccine. The titres of Pendik CE pathogen strain isolated from lamb (E(P)CK 4 ) and attenuated vaccine strain originated from lamb (E(P)CK 22 ) used in the study were TCID 50 10 6.50 and 10 7.00 /ml, respectively. In the study, to determine the pathogenicity of CE virus (E(P)CK 4 ) in rabbits, it was found that CE virus-specific hyperemia, vesicles and pustules were not observed and there was no increase in body temperature, and CE virus was not detected by PCR in the scabs located in the skin of back-waist regions. In the blood sera of rabbits treated with pathogen CE virus and vaccinated with CE vaccine virus, no antibodies were detected against CE virus. In the immunity study in lambs and kids vaccinated with CE vaccine (E(P)CK 22 ), it was found that the vesicles, pustules and scabs appeared on day 3 and the lesions healed on the 15 th day seen that CE vaccine virus protects lambs and kids against pathogen CE (E(P)CK 4 ) strain.

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“…Their daily body temperature was recorded. Kids were observed during the quarantine for clinical signs of CE and other diseases (Buddle and Pulford, 1984; Pye, 1990;Musser et al 2012).…”

Section: Vaccination Of Rabbits Lambs and Kids With Ce Vaccinementioning

confidence: 99%

“…Animals should be vaccinated with CE vaccines in the fight against CE disease. There are some kind of vaccine such as live attenuated in tissue culture and not fully attenuated and also produced in sheep are used for the prevention of CE disease, (Pye D, 1990;Musser et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Gülyaz¹,

Saraç

Hasöksüz³

et al. 2020

Etlik Veteriner Mikrobiyoloji Dergisi

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“…Four lambs to be used as controls (mock) were also scarified diagonally by 3-4 scratches with a diameter of 0.5-1 cm with a needle deep enough to pass the first layer in the hairless skin region of the hind legs. Three drops of PBS (50%) + glycerin (50%) were applied to the scarified areas and the animals were released after 3-4 seconds (Ergin and Köklü 1973;Buddle and Pulford, 1984;Pye 1990;Musser et al 2012). Body temperature of the animals were measured 2 times a day for 15 days.…”

Section: Vaccination Of Lambs: Lyophilized Ce (E(p)mentioning

confidence: 99%

“…Body temperatures of lambs were daily measured for 15 days. Following the challenge, vesicles, pustules and subsequent scabbing caused by virus reproduction were observed in the scarification areas on the skin of the control lambs, which were challenged at the same time with vaccinated lambs (Ergin and Köklü 1973;Buddle and Pulford, 1984;Pye, 1990;Musser et al 2012).…”

Section: Challenge Studies In Vaccinated Lambsmentioning

confidence: 99%

“…For the prevention of disease, attenuated tissue culture and vaccines that are not fully attenuated and produced in sheep are used (Pye D 1990;Musser et al 2012) The aim of this study was to adapt the CE vaccine strain to Madin Darby Bovine Kidney (MDBK) cell culture and to produce the vaccine, as well as determining the shelf life, stability, innocuity, and immune response in lambs.…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of Contagious Ecthyma vaccine strain to MDBK cell culture and immunity-stability studies in lambs

Gülyaz¹,

Saraç

Satir³

et al. 2020

Etlik Veteriner Mikrobiyoloji Dergisi

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Contagious ecthyma (CE) is a common viral infection in lambs and kids, and still maintains its importance in sheep and goat breeding. Attenuated, live and lyophilized CE vaccines adapted to cell culture are widely used in the fight against CE. The aim of this project was to adapt the CE vaccine strain to Madin Darby Bovine Kidney (MDBK) cell culture and to produce the vaccine, as well as determining the shelf life, stability, innocuity, and immune response in lambs.The titer of the vaccine virus adapted to the MDBK cell culture was determined to be TCID 50 10 6.5 / ml. In the innocuity study on mice and guinea pigs, no local or general reactions were observed. Body temperature of 10 lambs vaccinated by scarification was found to be between normal values (38.2-39.1°C). Neutralizing antibodies belonging to CE virus with VNT could not be detected in blood sera taken on 30 th day following the vaccination. As a result of challenge performed with pathogen (E(P)CK 5 ) CE virus, the protection was observed against CE infection in vaccinated lambs. In the stability study, the titers of lyophilized CE vaccine were found to be TCID 50 10 6.5 /ml from 1 to 15 months and 10 6.0 /ml at 18, 21 and 24 months during the 24-month storage period at +4/+8°C. It was determined that the vaccine virus remained as stable at TCID 50 10 6.5 /ml titer for 12 hours at 30, 33, 37 and 40°C and after 12 hours, the virus titer regressed to TCID 50 10 5.75 /ml at 42°C and 45°C. This study concluded that CE vaccines could be produced in MDBK cell cultures by adapting CE virus to MDBK cell culture.

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Genetic diversity of Orf virus isolated from sheep in Uruguay

Olivero

Reolón²,

Arbiza³

et al. 2018

Arch Virol

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Orf virus (ORFV) is the etiological agent of contagious ecthyma, a disease widely spread in the world that occasionally causes zoonotic infections. This work is the first molecular characterization of ORFV in Uruguay, where we analyzed twenty-one sheep samples, eighteen of which were recovered from thirteen ORFV outbreaks that occurred during 2004 to 2011 as well as three strains from a national vaccine. Phylogenetic analysis and the derived amino acid sequences from the B2L gene suggest that the Uruguayan virus do not form a unique cluster, with most of them displaying similarities with worldwide ORFV isolates as well as our vaccine strains.

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Evaluation of homologous and heterologous protection induced by a virulent field strain of orf virus and an orf vaccine in goats

Abstract: Vaccination did not result in cross protection for the 2 strains of orf virus. This may have been attributable to antigenic differences and may be a factor in outbreaks of contagious ecthyma in vaccinated goats.

Cited by 10 publications

References 25 publications

Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Adaptation of Contagious Ecthyma vaccine strain to MDBK cell culture and immunity-stability studies in lambs

Genetic diversity of Orf virus isolated from sheep in Uruguay

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