Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Fazelipour, Simin; Tootian, Zahra; Saremi, Zahra; Shafii, Minoo; Sheibani, Mohammad Taghi; Kiaei, Seyed Babak; Kiumarsi, Mehrnoush; Assadi, Fardin

doi:10.3906/sag-1301-109

Cited by 13 publications

(17 citation statements)

References 26 publications

(31 reference statements)

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“…The diameters of fifty seminiferous tubules from each section were randomly examined by using an ocular light micrometer (27). The thicknesses of averagely fifty points of tunica albuginea from each section were measured by a micrometer.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats

Ghanbari

Nejati

Khazaei

2016

IJRM

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Background:Diabetes is the most common endocrine disease. It has adverse effects on male reproductive function. Royal Jelly (RJ) has antioxidant and anti-diabetic effects and show protective effects against diabetes.Objective:This study was conducted to evaluate the effect of RJ on histopathological alterations of the testicular tissue in streptozotocin (STZ)-induced diabetic rats. Materials and Methods:In this experimental study, 28 adult Wistar rats were randomly divided into control (C), royal jelly (R), diabetic (D) and RJ-treated diabetic (D+R) groups. Diabetes was induced by a single intraperitoneal injection of STZ at 50 mg/kg body weight (BW). The rats from the R and D+R groups received daily RJ (100 mg/kg BW) for 6 wks orally. Hematoxylin-Eosin staining was used to analyze histopathological changes including: tunica albuginea thickness (TAT), seminiferous tubules diameter (STsD), Johnsen’s score, tubular differentiation index (TDI), spermiogenesis index (SPI), Sertoli cell index (SCI), meiotic index (MI), and mononuclear immune cells (MICs) in testes. The antioxidant status was examined by evaluating testicular levels of ferric reducing antioxidant power (FRAP) and catalase (CAT) activity.Results: Histological results of the testis from diabetic rats showed significant decrease in STsD, Johnsen’s score, TDI, SPI, SCI and MI, and significant increase in TAT and MICs, while administration of RJ significantly reverted these changes (p<0.05). RJ treatment markedly increased activity of CAT and FRAP. There were significant differences in FRAP levels among C (13.0±0.5), RJ (13.4±0.3), D (7.8±0.6) and D+R (12.4±0.7) groups (p<0.05).Conclusion:RJ improved diabetes-induced impairment in testis, probably through its antioxidant property.

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Section: Methodsmentioning

confidence: 99%

Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats

Ghanbari

Nejati

Khazaei

2016

IJRM

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“…The equivalent dose of MPH in rats to a clinically relevant dose in humans is 1.7 to 5.5 mg/kg (Solanto, 2000). However, doses higher than this range (10–30 mg/kg) were administered for rats in some studies (Fazelipour et al, 2012, 2014; Komatsu et al, 2012), whereas a median to high dose (5–10 mg/kg) was used in another study (Cansu et al, 2011). In juvenile rhesus monkeys with the observed pubertal delay, MPH was administered with 2.5 to 12.5 mg/kg twice per day since approximately 2 y old, for a total duration of 40 months (Mattison et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Avital et al (2011) reported that environment enrichment in adolescent rats is a moderating factor between MPH administration and corticosterone and testosterone levels in their adulthood. By contrast, some studies have indicated that perinatal or postnatal MPH exposure does not significantly affect serum testosterone levels in rats (Ferguson and Boctor, 2010; Panos et al, 2014) and can even increase spermatogonia and testosterone levels (Fazelipour et al, 2014). …”

Section: Introductionmentioning

confidence: 95%

Does Methylphenidate Reduce Testosterone Levels in Humans? A Prospective Study in Children with Attention-Deficit/Hyperactivity Disorder

Wang

Chou

et al. 2016

IJNPPY

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Background:Animal studies and case reports have suggested that methylphenidate exerts adverse effects on gonadal hormones. This study aimed to determine whether methylphenidate alters testosterone levels in children with attention-deficit/hyperactivity disorder through comparison of those with or without methylphenidate treatment.Methods:This 4-week, nonrandomized, prospective study conducted in Taiwan included 203 attention-deficit/hyperactivity disorder patients with a mean age of 8.7 years (boys: 75.8%). After the initial recruitment, 137 received daily methylphenidate treatment (medicated group) and 66 were assessed through naturalistic observation (nonmedicated group). The saliva samples of attention-deficit/hyperactivity disorder patients were used to quantify testosterone levels at baseline and the endpoint by using the chemiluminescence immunoassay. At the 4th week, 86 patients in the medicated group and 46 patients in the nonmedicated group were eligible for statistical analyses.Results:During the study period, salivary testosterone levels did not significantly change in the medicated group (P=.389) or in the nonmedicated group (P=.488). After correction for the potential confounding effects of age and sex, salivary testosterone levels still remained unchanged in the medicated and nonmedicated groups during the 4-week follow-up. In the medicated group, changes in salivary testosterone levels over 4 weeks were not significantly correlated with the methylphenidate daily dose (mean daily dose: 18.1 mg).Conclusions:Findings suggest that short-term treatment with methylphenidate at usual doses does not significantly alter salivary testosterone levels in attention-deficit/hyperactivity disorder patients. Future studies should clarify whether long-term methylphenidate treatment disrupts testosterone production as well as the function of the reproductive system.

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“…Seminiferous tubules (STs) diameters, germinal cell layer thicknesses and number of spermatogonia were measured using an ocular micrometer in a light microscope as previously described. 13 Additionally, spermatogenesis in the tissue samples of the testes was evaluated according to the Johnsen scoring system 14 as shown in Table 1.…”

Section: Il-6 and Tnf-α Measurementmentioning

confidence: 99%

The Improvement Effects of Gordonia bronchialis on Male Fertility of Rats with Diabetes Mellitus Induced by Streptozotocin

et al. 2019

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Background: This study evaluated the possible protective effects of Gordonia bronchialis (Gb) on oxidative stress and some subsequent alterations on testis from rats undergoing an experimentally induced type 1 diabetes. Methods: A total of 40 male rats were randomly divided into four groups of ten. Diabetes was induced by injection of 55 mg/kg streptozotocin in 30 rats. Oral administration of Gb at dose of 105 (low dose) and 107 (high dose) CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and healthy control groups, respectively. The blood and testicular tissue samples were taken on the 14th and 21st days post treatment for biochemical and histopathological evaluations. Results: Significant differences were found in blood glucose level, insulin, IL-6 and TNF-α values together with catalase and superoxide dismutase activities and malondialdehyde level in the diabetic group in comparison with healthy and Gb recipient groups. Moreover, the histopathological lesions observed in the diabetic rats mainly included basement membrane thickening, decreased number of Sertoli cells, and severe reduction of spermatogenesis markedly attenuated in Gb-treated rats. Conclusion: Taken together, it seems that oral administration of Gb could ameliorate testicular damage associated with some related parameters in the diabetic animal model.

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Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Cited by 13 publications

References 26 publications

Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats

Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats

Does Methylphenidate Reduce Testosterone Levels in Humans? A Prospective Study in Children with Attention-Deficit/Hyperactivity Disorder

The Improvement Effects of Gordonia bronchialis on Male Fertility of Rats with Diabetes Mellitus Induced by Streptozotocin

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