Evaluation of Hepatoprotective activity of Ethanolic Extract of Nymphaea alba Linn Flower in experimental rats

Paharia, Ashish K.; Pandurangan, A. G.; Gupta, Manoj Kumar

doi:10.7439/ijbr.v4i7.326

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…It consists of various phytochemical compounds, for example, alkaloids, polyphenolic compounds, flavonoids, tannic acid, glycosides, gallic acid, and sterols. Most commonly it is used to cure gonorrhoea, diarrhea, and leucorrhoea because of antibacterial potential of this plant [ 9 ]. Various in vivo studies of Nymphaea alba reported that this plant has antidiabetic [ 10 ], antidiarrheal [ 11 ], hepatoprotective, antioxidant, and anti-inflammatory activities [ 12 ], intestinal α -glucosidase inhibition and toxicity [ 13 ], anxiolytic activity [ 14 ], and anticarcinogenic effect [ 8 ], whereas, in vitro studies also revealed antioxidant [ 15 ] and antibacterial activity [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Antilisterial Effect ofRosa damascenaandNymphaea albainMus musculus

Batool

Kalsoom

Akbar

et al. 2018

BioMed Research International

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The present study was proposed to investigate the toxicological and prophylactic potential of ethanolic extracts of Rosa damascena and Nymphaea alba and their mixture in albino mice. For toxicity study, three different doses of plant extracts were orally administrated to three groups of mice for 14 successive days. Blood biochemistry and histological examinations of liver and kidney revealed that these extracts had no harmful effects up to 1000 mg/kg. To determine the prophylactic effects of Rosa damascena, Nymphaea alba, and their mixture, an infection model of Listeria monocytogenes was established in a pilot study. Establishment of infection was confirmed by changes in haematological parameters and reisolation of Listeria monocytogenes from different tissues. Results showed that these extracts alone or in combination could restrict the growth of Listeria monocytogenes in different organs. Neutrophils were high in positive control group but remained in normal range in all treated groups. Listeria monocytogenes was recovered in low numbers from animals treated with extract of single plant but was negligible in group treated with mixture of extract of plants. Platelets count was increased in treated groups as compared to control. Results confirmed that these extracts are potent source of antimicrobial compounds and that they have synergistic effect in combined form.

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Section: Introductionmentioning

confidence: 99%

Antilisterial Effect ofRosa damascenaandNymphaea albainMus musculus

Batool

Kalsoom

Akbar

et al. 2018

BioMed Research International

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“…The broad range of traditional uses along with the previous reports concerning the hepatoprotective effect of N. alba flowers [7] as well as other Nymphaea species [15], and the absence of any reports about the phytochemical profile of N. alba leaf, aroused our interest in N. alba as a source of bioactive compounds. This study represents the first detailed chemical investigation of N. alba leaf that demonstrates the presence of a variety of free and conjugated forms of ellagic acid and ellagitannins tentatively identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS) in the aqueous ethanolic extract.…”

Section: Introductionmentioning

confidence: 99%

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr

El-Naa

Zaghloul

et al. 2017

BMC Complement Altern Med

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Background Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders.MethodsThe aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator; tumour necrosis factor (TNF)-α were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performedResultsFifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-α in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression.ConclusionThese results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-α, might be the possible hepatoprotective mechanisms.

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“…Previous studies have shown the presence of flavonoids and phenolic compounds in ethanolic extract of Nymphaea alba. 3 The presence of these compounds might be responsible for the antioxidant activity. Hepatoprotection offered by Nymphaea alba ethanolic extract could be attributed to these constituents, since antioxidants have shown hepatoprotective activity in previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…The flowers and leaves contain various phytoconstituents such as alkaloids, carbohydrates (polysaccharides), glycosides, steroids, flavonoids, tannin, and phenolic compound. 3 Previously it has been reported to be used as an anti-inflammatory, demulcent, analgesic, astringent, cardiotonic, sedative and aphrodisiac. It is also useful in the treatment of anxiety, insomnia, and similar disorders.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Potentials of Nymphaea Alba on Hepatotoxicity and Oxidative Damage Induced by Chronic Exposure to Isoniazid in Rats

Nasiruddin¹,

Khan²,

Arif³

2019

Int Res J Pharm

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Objective: Evaluation of hepatoprotective and antioxidant potentials of ethanolic extract of Nymphaea alba against chronic exposure to isoniazid in rats. Material and Methods: Chronic administration of isoniazid (50 mg/kg) orally for 28 days lead to hepatotoxicity in rats. Silymarin (50 mg/kg) and ethanolic extract of Nymphaea alba flowers and leaves (200 mg/kg and 400 mg/kg) were given orally for 31 days. Isoniazid was given to all rats except normal control from 4 th to the 31 st day of the study. On the 32 nd day, all animals were sacrificed, blood samples were collected for liver function tests and antioxidant study. Liver tissues were subjected to histopathological examination. Results: Chronic administration of isoniazid resulted in a significant elevation in liver enzymes and oxidative stress markers in rats. The extracts in 200 mg/kg and 400 mg/kg doses significantly decreased the isoniazid-induced elevation of liver enzymes (AST, ALT, ALP and serum bilirubin) as well as oxidative stress markers (catalase, GSH, and MDA) in rats. Histological findings supported the changes in biochemical parameters. Conclusion: Ethanolic extract of Nymphaea alba (NAEE) in 200 mg/kg and 400 mg/kg dose possess hepatoprotective and antioxidant potential. NAEE has ameliorative potentials against chronic isoniazid exposure induced hepatotoxicity in rats.

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Evaluation of Hepatoprotective activity of Ethanolic Extract of Nymphaea alba Linn Flower in experimental rats

Cited by 8 publications

References 13 publications

Antilisterial Effect ofRosa damascenaandNymphaea albainMus musculus

Antilisterial Effect ofRosa damascenaandNymphaea albainMus musculus

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Ameliorative Potentials of Nymphaea Alba on Hepatotoxicity and Oxidative Damage Induced by Chronic Exposure to Isoniazid in Rats

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