Evaluation of gut modulatory and bronchodilator activities of Amaranthus spinosus Linn.

Abstract

BackgroundThe aqueous-methanolic extract of Amaranthus spinosus (A. spinosus Linn.,) whole plant, was studied for its laxative, spasmolytic and bronchodilator activities to validate some of its medicinal uses.MethodsThe crude extract of A. spinosus was studied in-vivo for bronchodilator and laxative activities and in-vitro using isolated tissue preparations which were mounted in tissue baths assembly containing physiological salt solutions, maintained at 37°C and aerated with carbogen, to assess the spasmolytic effect and to find out the possible underlying mechanisms.ResultsIn the in-vivo experiments in mice, the administration of A. spinosus increased fecal output at doses of 100 and 300 mg/kg showing laxative activity. It also inhibited carbachol-induced bronchospasm in anesthetized rats at 1, 3, 10 and 30 mg/kg indicative of bronchodilator activity. When tested on isolated gut preparations, the plant extract showed a concentration-dependent (0.01-10.0 mg/ml) spasmogenic effect in spontaneously contracting rabbit jejunum and guinea-pig ileum. The spasmogenic effect was partially blocked in tissues pretreated with atropine (0.1 μM). When tested on K+ (80 mM)-induced sustained contractions in isolated rabbit jejunum, the plant extract caused complete relaxation and also produced a shift in the Ca++ concentration-response curves (CRCs) towards right, similar to diltiazem. In rabbit trachea, the plant extract completely inhibited K+ (80 mM) and carbachol (CCh, 1 μM)-induced contractions at 1 mg/ml but pretreatment of tissue with propranolol (1 μM), caused around 10 fold shift in the inhibitory CRCs of the plant extract constructed against CCh-induced contraction. The plant extract (up to 0.3 mg/ml) also increased both force and rate of spontaneous contractions of isolated guinea-pig atria, followed by relaxation at higher concentration (1.0-5.0 mg/ml). The cardio-stimulant effect was abolished in the presence of propranolol, similar to that of isoprenaline. Activity-directed fractionation revealed that the spasmolytic component(s) was separated in the organic fraction, whereas the spasmogenic component was concentrated in the aqueous fraction.ConclusionThese results indicate that A. spinosus possesses laxative activity partially mediated through cholinergic action. The spasmolytic effect was mediated through calcium channel blocking (CCB), while bronchodilator activity through a combination of β-adrenergic and CCB pathways, which may explain the traditional uses of A. spinosus in gut and airways disorders.

Medicinal Plants used during Antenatal Care by Pregnant Women in Eastern Uganda

“…D. laxata has antiinflammatory and antinociceptive activities in vivo 45 . A. spinosus has been shown to have laxative and bronchodilatory effects in vitro 47 . A. spinosus contains rutin 46 , which is widely acknowledged for its nutraceutical effects and several pharmacological effects 48 such as antifatigue 49 , immunostimulatory 50 among others.…”

Studies on antidiarrhoeal, antispasmodic and bronchodilator activities of Operculina turpethum Linn

“…To assess whether the spasmolytic activity of the test substance was mediated through Ca ++ channel blockade (CCB), the high K + (80 mM), as KCl, was used to depolarize the preparations [19]. The high K + (80 mM) was added to the tissue bath, which produced a sustained contraction.…”

Calcium channels blocked activity: Providing the basis for medicinal use of <i>Abies pindrow</i> in diarrhea and bronchitis

“…In our previous studies, involvement of calcium channel blocking activity was observed as underlying cause of spasmolytic effect of crude extract (urRahaman et al, 2013;Chaudhary et al, 2012). To explore possible mechanisms behind spasmolytic effect, high K + (80 mM) and carbachol (1 µM)-induced contraction on isolated rabbit jejunum were challenged with the extract.…”

“…All the in vitro experiments were performed as per protocols previously described by (Chaudhary et al, 2012).…”