Evaluation of glycine betaine as an inhibitor of dissolved dimethylsulfoniopropionate degradation in coastal waters

Kiene, RP; Gerard, Ghislain

doi:10.3354/meps128121

Cited by 44 publications

(52 citation statements)

References 21 publications

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“…5). This high efficiency is consistent with the view that dissolved DMSP is efficiently consumed by bacteria in coastal waters (Kiene & Service 1991, Kiene & Gerard 1995, yet higher than the conversion efficiencies measured in some studies (Kiene 1996b, van Duyl et al 1998, Simó & Pedrós-Alió 1999. In contrast, DMSP d and DMS did not correlate with DMSP s (Fig.…”

Section: Discussion Dmsp S Dmsp D and Dmssupporting

confidence: 79%

“…The range of DMSP s observed in the present study was comparable to that in 4 other estuaries (Iverson et al 1989), but considerably less than in some other coastal waters (Kiene & Service 1991, Kiene & Gerard 1995, Kiene 1996a). We observed a stronger correlation between DMSP d and DMS in the present study than that derived by Kettle et al (1999) from a global database.…”

Section: Discussion Dmsp S Dmsp D and Dmssupporting

confidence: 61%

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Seasonal distribution of DMSP among seston, dissolved matter and zooplankton along a transect in the Long Island Sound estuary

Tang¹,

Rogers²,

Dam³

et al. 2000

Mar. Ecol. Prog. Ser.

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We studied the seasonal distribution of dimethylsulfoniopropionate (DMSP) among seston, dissolved matter and zooplankton along a transect in eastern Long Island Sound. The seston DMSP concentration (1 to 52 nM) was comparable to that reported for some estuaries. Most of the seston DMSP was derived from particles <10 µm. Seston DMSP concentration did not correlate with water temperature or salinity. Most of the seston DMSP appeared to have originated from phytoplankton. Both dissolved DMSP and dimethyl sulfide (DMS) concentrations remained low (< 3 nM) and were highly correlated to each other (r = 0.83, p < 0.01). Assuming a steady state condition, the conversion efficiency from dissolved DMSP to DMS was estimated to be 76%. On the other hand, seston DMSP concentration did not correlate with dissolved DMSP, implying that the accumulation of seston DMSP and dissolved DMSP were uncoupled. Four types of copepods plus several other types of zooplankters contained DMSP. The copepod Temora longicornis contained 2.8 nmol DMSP per individual, the highest among the zooplankters. For most of the year, zooplankton were a negligible component of particulate DMSP in the water column. However, in months when T. longicornis appeared in high abundance, zooplankton represented 14 to 72% of the total particulate DMSP. Estimated copepod body DMSP concentrations were orders of magnitude higher than seston and dissolved DMSP concentrations; thus, copepod bodies represent a sparse, but highly concentrated source of particulate DMSP.

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Section: Discussion Dmsp S Dmsp D and Dmssupporting

confidence: 79%

Section: Discussion Dmsp S Dmsp D and Dmssupporting

confidence: 61%

Seasonal distribution of DMSP among seston, dissolved matter and zooplankton along a transect in the Long Island Sound estuary

Tang¹,

Rogers²,

Dam³

et al. 2000

Mar. Ecol. Prog. Ser.

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“…5a), whereas incubations in warmer waters often show increasing removal of DMS over time, likely due to growth of consumers (see for example Therefore, we hypothesize that in colder waters, the lower temperature and kinetic sensitivity of eukaryotic DMS production may at times combine to outstrip DMS consumption, leading to the high pulses of DMS observed. In this study we were not able to separate bacterial DMS production from eukaryotic production pathways: attempts to inhibit bacterial DMSPd uptake by additions of 50 PM glycine betaine did not yield consistent results, possibly due to its transient effect (Kiene & Gerard 1995) in our relatively long incubat i o n~. We also did not explicitly examine the effects of temperature on DMS production and consumption.…”

Section: Limitations On Microbial Dms Consumption In Cold Watersmentioning

confidence: 81%

“…This was added by pipette to incubation bottles to final DMSP concentrations of 10 to 50 nM. Glycine betaine (GB, Aldrich) was added at 50 pM from a 100 mM stock solution to inhibit DMSPd uptake (Kiene & Gerard 1995).…”

Section: Dark and Light Incubations + Dmdsmentioning

confidence: 99%

Microbial consumption and production of dimethyl sulfide (DMS) in the Labrador Sea

Wolfe¹,

Levasseur²,

Cantin³

et al. 1999

Aquat. Microb. Ecol.

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We examined microbial production and consumption of dimethyl sulfide (DMS) in Labrador Sea surface waters ranging in temperature from -0.1 to 6.g°C. 200 nM dimethyl disulfide (DMDS) was used to inhibit DMS consumption. We also studied DMS consumption kinetics by additions of 5 to 50 nM DMS, DMS production from added dirnethylsulfoniopropionate (DMSP), and DMS production and consumption during zooplankton grazlng. During the cruise, DMS concentrations were low, ranging from 1 to 7 nM throughout the study area, which ~ncluded a bloom of the colonial haptophyte alga Phaeocystjs poucheti~. DMDS additions often revealed rapid DMS production and consumption (up to 5 nM d-') and very rapid turnover ( < l to 3 d), s~milar to rates found in coastal waters at much higher temperatures. There was no clear effect of temperature on DMS consumption; rather, DMS consumption appeared to be tightly coupled w~t h production. Turnover was most rapid at low DMS concentrations, and DMS consumption was stlrnulated by additions of DMS, or by increased DMS production from additions of dissolved DMSP. DMDS addit~ons to zooplankton grazing incubations revealed rapid gross DMS production and consumption which were nearly balanced, resulting in net steady-state DMS patterns. DMDS did not dlfect production or grazing of algal pigments or DMSP. DMS consumption saturated at 18 to 32 nM [DMS] and saturation kinetics were surular within the photic zone, but consumption was near-zero at greater depths. We suggest that DMS consumption likely saturates more easily than microbial DMS production from DMSP, and this, combined with temperature limitation on the growth of prokaryotic DMS consumers, may lead to the periodic buildup of high DMS concentrations previously observed in polar and subpolar waters.

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“…GB and TMA inhibited MeBr degradation in strains FV, LIS-3 and TNA (Table 3). Kiene & Gerard (1995) reported a similar effect of GB on DMSP use, and Wolfe & Kiene (1993) found that GB inhibited DMS consumption. In the strains isolated in this study, GB and TMA seemed to function as competitive substrates for MeBr consumption, while DMS greatly stimulated MeBr degradation (Table 3).…”

Section: Discussionmentioning

confidence: 79%

Metabolism of methyl bromide and dimethyl sulfide by marine bacteria isolated from coastal and open waters

Hoeft

Rogers²,

Visscher

2000

Aquat. Microb. Ecol.

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Marine methylotrophic bacteria that consumed methyl bromide (MeBr) and/or dunethyl sulfide (DMS) were isolated: 4 strains from Long Island Sound, USA (LIS) and 1 from the tropical North Atlantic (TNA). Substrates used for enrichment were DMS, trimethylarnine (TMA) or TMA plus MeBr. Attempts to obtain isolates on MeBr as the sole source of carbon and energy were unsuccessful. Three of the isolates used MeBr at rates that were higher than chemical loss, and one of these, isolated from coastal LIS, could use this C,-substrate for biomass production but only when other carbon sources were available simultaneously. Microbial consumption stopped when the MeBr concentration was higher than 0.5 mM and, similarly, when the DMS concentration was higher than 5 mM. The presence of DMS greatly enhanced MeBr consumption in cell suspensions. Inhibitor studies showed that DMS and MeBr were used by the same metabolic pathway in 2 LIS isolates, one of which &splayed hydroxypyruvate reductase activity, ~ndicatlve of the serine pathway of carbon assimilat~on. A third isolate obtained from the TNA that used MeBr did not use DMS, whereas 2 additional LIS strains isolated on DMS did not use MeBr. Therefore, MeBr uthzation is not a common trait of DMS-degrading bacteria. MeBr concentrations in the marine environment are in the pM range and are the highest In coastal waters. This study indicates that growth on MeBr alone is unlikely to support bactenal growth and that removal of MeBr may be a cometabolic phenomenon. However, regardless of growth, consumption by manne bacteria may h u t the flux of MeBr to the atmosphere.

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Evaluation of glycine betaine as an inhibitor of dissolved dimethylsulfoniopropionate degradation in coastal waters

Cited by 44 publications

References 21 publications

Seasonal distribution of DMSP among seston, dissolved matter and zooplankton along a transect in the Long Island Sound estuary

Seasonal distribution of DMSP among seston, dissolved matter and zooplankton along a transect in the Long Island Sound estuary

Microbial consumption and production of dimethyl sulfide (DMS) in the Labrador Sea

Metabolism of methyl bromide and dimethyl sulfide by marine bacteria isolated from coastal and open waters

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