Evaluation of Glucosinolate Variation in a Collection of Turnip (Brassica rapa) Germplasm by the Analysis of Intact and Desulfo Glucosinolates

Lee, Jun Gu; Bonnema, Guusje; Zhang, Ningwen; Kwak, Jung Ho; Vos, Ric C. H. de; Beekwilder, Jules

doi:10.1021/jf400890p

Cited by 59 publications

(57 citation statements)

References 33 publications

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“…Most of the GLs detected in the turnip samples were the same as those found in a previous report. 9 The total amount of GL in these two turnips was below the average GL amount found in the 71 radish accessions. Two clear differences were observed between the radish and turnip samples.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 65%

Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources

Yi¹,

Lim²,

Chae

et al. 2015

J. Agric. Food Chem.

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Radish (Raphanus sativus L.), a root vegetable, is rich in glucosinolates (GLs), which are beneficial secondary metabolites for human health. To investigate the genetic variations in GL content in radish roots and the relationship with other root phenotypes, we analyzed 71 accessions from 23 different countries for GLs using HPLC. The most abundant GL in radish roots was glucoraphasatin, a GL with four-carbon aliphatic side chain. The content of glucoraphasatin represented at least 84.5% of the total GL content. Indolyl GL represented only 3.1% of the total GL at its maximum. The principal component analysis of GL profiles with various root phenotypes showed that four different genotypes exist in the 71 accessions. Although no strong correlation with GL content and root phenotype was observed, the varied GL content levels demonstrate the genetic diversity of GL content, and the amount that GLs could be potentially improved by breeding in radishes.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 65%

Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources

Yi¹,

Lim²,

Chae

et al. 2015

J. Agric. Food Chem.

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“…It is out of the scope of this study to compare or improve separation and detection methods but it should be noted that major glucosinolates in this study were accurately measured by a HPLC–UV method adapted from Herzallah and Holley [19]. For examination of low abundance glucosinolates, and to avoid any potential inaccuracies due to contamination it is advised that an alternative HPLC method such as those suggested in Lee et al or Forster et al be used instead [26, 32]. …”

Section: Extractionmentioning

confidence: 99%

Development of an efficient glucosinolate extraction method

et al. 2017

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BackgroundGlucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. There is also increasing interest in the biofumigant properties of toxic glucosinolate hydrolysis products as a method to control agricultural pests. Evaluating biofumigation potential requires rapid and accurate quantification of glucosinolates, but current commonly used methods of extraction prior to analysis involve a number of time consuming and hazardous steps; this study aimed to develop an improved method for glucosinolate extraction.ResultsThree methods previously used to extract glucosinolates from brassicaceae tissues, namely extraction in cold methanol, extraction in boiling methanol, and extraction in boiling water were compared across tissue type (root, stem leaf) and four brassicaceae species (B. juncea, S. alba, R. sativus, and E. sativa). Cold methanol extraction was shown to perform as well or better than all other tested methods for extraction of glucosinolates with the exception of glucoraphasatin in R. sativus shoots. It was also demonstrated that lyophilisation methods, routinely used during extraction to allow tissue disruption, can reduce final glucosinolate concentrations and that extracting from frozen wet tissue samples in cold 80% methanol is more effective.ConclusionsWe present a simplified method for extracting glucosinolates from plant tissues which does not require the use of a freeze drier or boiling methanol, and is therefore less hazardous, and more time and cost effective. The presented method has been shown to have comparable or improved glucosinolate extraction efficiency relative to the commonly used ISO method for major glucosinolates in the Brassicaceae species studied: sinigrin and gluconasturtiin in B. juncea; sinalbin, glucotropaeolin, and gluconasturtiin in S. alba; glucoraphenin and glucoraphasatin in R. sativus; and glucosatavin, glucoerucin and glucoraphanin in E. sativa.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0164-8) contains supplementary material, which is available to authorized users.

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“…The pellet was re-extracted once following the same procedure, and the supernatants were combined. Desulfoglucosinolates were then prepared and quantitatively determined by ultra-performance liquid chromatography (UPLC) using purified sulphatase isolated from H. pomatia according to Lee et al (2013). Briefly, the extract was loaded onto a Mini Biospin chromatography column (Bio-Rad) containing 0.5 mL of DEAE-Sephadex A 25 which was preactivated with 0.1 M sodium acetate (pH 4.0).…”

Section: Glucosinolate Analysismentioning

confidence: 99%

Seasonal variation in phytochemicals and antioxidant activities in different tissues of various Broccoli cultivars

Bhandari¹,

Kwak²

2014

Afr. J. Biotechnol.

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Florets, leaves, and stems of twelve commercial broccoli cultivars grown in the spring and fall seasons at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Suwon, South Korea were evaluated for glucosinolates, vitamin C, total phenol, and total flavonoid contents and antioxidant activity. The levels of all phytochemicals and antioxidant activity were significantly influenced by cultivar (C), plant part (P), and growing season (S). Among the glucosinolates, glucoraphanin and glucobrassicin were the major constituents. The highest total glucosinolate content was found in the florets of plants grown in both seasons. Phenols and flavonoids were highest levels in leaves, while vitamin C was highest in stems, suggesting that broccoli leaves and stems may be good sources of such phytochemicals. The levels of all phytochemicals were generally higher in florets in the spring than in the fall, but were higher in leaves and stems during the fall than the spring. Furthermore, higher cultivar-dependent and tissue-dependent variation was observed in the spring than in the fall. Total phenol content exhibited a strong positive correlation (r = 0.674**) with antioxidant activity, followed by total flavonoid content (r = 0.497**), indicating their significant contribution to total antioxidant activity.

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Evaluation of Glucosinolate Variation in a Collection of Turnip (Brassica rapa) Germplasm by the Analysis of Intact and Desulfo Glucosinolates

Cited by 59 publications

References 33 publications

Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources

Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources

Development of an efficient glucosinolate extraction method

Seasonal variation in phytochemicals and antioxidant activities in different tissues of various Broccoli cultivars

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