Evaluation of Genotoxicity of 4-n-Nonylphenol using Vicia faba L.

Adam, F. I. M.; El-Ashry, Zeinab M.

doi:10.3923/jbs.2010.368.372

Cited by 15 publications

(9 citation statements)

References 4 publications

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“…Also, sticky chromosomes can cause loss of genetic material and subsequently, the cell-division process occurs irregularly, with some chromosomes not adhering to the assembled chromosomal complex and being lost during the cell cycle. Laggard's chromosomes induction is due to delayed termination of chromosome end or because of failure of chromosomal movement and attributed to the spindle apparatus [46] [47].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Roșculete¹,

Olaru²,

Roșculete³

et al. 2020

AJPS

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With the unprecedented development of the food industry, food preservatives have gained a leading role in food processing. In this study, investigations were carried out to assess the cytological effects of potassium metabisulphite (PMB) to onion (Allium cepa), one of the most used plants for determining the cytotoxic and genotoxic effects of different chemicals. Meristematic roots of A. cepa were treated with PMB solutions in different concentrations, ranging from 10 mg/l to 35 mg/l for 6, 12 and 24 h alongside an untreated control. The quantified parameters for the different concentrations of the PMB were mitotic index (MI %), mitosis phases index (PI %) and total abnormalities index (TAI %) meaning chromosomal aberrations and nuclear abnormalities. The results indicated that PMB reduced MI in A. cepa with increasing the concentrations and time exposure as compared with the untreated control. Thus, at concentration of 10 -35 mg/l PMB, these values were reduced from 8.34% to 4.81% (6 h); 7.18% to 2.35% (12 h) and 4.12 to 1.43 (24 h). Also, the TAI value increased with increasing PMB concentrations and time: 0.51% to 9.98% (6 h), 7.11% to 19.84% (12 h) and 17.79 to 41.21 (24 h). The types of abnormalities induced by PMB in A. cepa meristematic cells were micronucleus, C-metaphase, star anaphase, stickiness, laggards, fragments, binucleated cells and pulverised nucleus. These alarming findings indicate the cytotoxic and genotoxic effect of PMB to A. cepa and suggest necessity to adopt more natural alternatives for food preservation in the future.

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Section: Discussionmentioning

confidence: 99%

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Roșculete¹,

Olaru²,

Roșculete³

et al. 2020

AJPS

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“…Disturbed metaphase may be the result of disturbances in the spindle apparatus or due to inhibition of spindle formation (Hsieh et al 2012;Cuyacot et al 2014;Tripathy and Rao 2015). Adam and El-Ashry (2010) attributed macro and micro cells to the failure of normal organization and function of spindle apparatus.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Sumitha

Thoppil

2015

Cytotechnology

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Hyptis suaveolens and Leucas indica, two common weeds were selected for the present study, to reveal their probable cytotoxic potential. The meristematic root tips of Allium cepa were used for testing the cytotoxic property of the aqueous leaf extracts containing both polar and non-polar compounds, and that containing polar compounds alone, at different concentrations (0.125, 0.25, 0.5, 1 and 2 %) and at different time durations, using distilled water as negative control. Mitotic squash preparations were made using a standard protocol. The mitotic index of the treated root tip cells was found to be decreasing and the abnormality percentage was found to be increasing with increase in extract concentration when compared with the control. Maximum cytotoxicity was observed in the extract containing both polar and non-polar compounds. Both the tested plants were found to be cytotoxic. The abnormalities noticed were of both clastogenic (nuclear lesions, nuclear fragmentation, etc.) and non-clastogenic (aberrant cell wall formation at cytokinesis, ball metaphase, etc.) types. Both plant extracts were found to significantly (P \ 0.05) inhibit root growth of Allium cepa with an EC 50 value of 1.92 % (R 2 = 0.594) for Hyptis and 1.58 % (R 2 = 0.757) for Leucas. The results were also analysed statistically by using analysis of variance followed by appropriate post hoc tests. These two weeds are aromatic plants comprising of essential oils that are volatile, natural complex compounds characterized by a strong odour and formed as secondary metabolites. In nature, essential oils play an important role in the protection of the plants as insecticides by reducing their appetite for such plants. When specifically targeted the concept of effectively exploiting these weeds for the formulation of herbal insecticides/ pesticides may be possible in the near future.

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“…Efeitos estimulatórios foram observados sobre a prófase nas menores concentrações do EEB e frações FH, FCL e FR. O predomínio de efeitos inibitórios sobre as diferentes fases da mitose em células meristemáticas nas raízes das plântulas de alface levam a uma diminução no crescimento, tendo em vista que esta região meristemática é a responsável pela produção de novas células e está estreitamente relacionada com o crescimento das raízes (Adam & El-Ashry, 2010).…”

Section: Alface -L Sativa Lunclassified

Estudo do potencial fitotóxico de extratos de Bauhinia ungulata L. sobre a divisão celular e atividade enzimática em plântulas de alface

Paula

Canteli

Silva

et al. 2015

Rev. bras. plantas med.

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RESUMO:Este trabalho visou avaliar a ação fitotóxica de extrato e frações obtidas das folhas de Bauhinia ungulata L sobre a divisão celular e atividade das enzimas α-amilase, catalase, peroxidase e polifenoloxidase em plântulas de alface (Lactuca sativa, var Grand rapis). Sementes de alface foram expostas às concentrações de 250, 500 e 1000 µg.mL -1 do extrato de Bauhinia ungulata e frações do mesmo, em condições ambientais controladas. Após três dias de protusão radicular, as raízes das plântulas foram cortadas e submetidas a preparo específico para visualização e contagem do número de células em cada fase mitótica. No sétimo dia, as amostras foram submetidas à avaliação da atividade enzimática utilizando técnica específica para cada enzima e leitura espectrofotométrica. Na análise da divisão celular observou-se que houve redução no número de células em mitose nas raízes das plântulas testadas, e a fração acetato de etila apresentou maior efeito inibitório sobre a divisão celular. O efeito sobre as enzimas que demonstram alterações no sistema de defesa antioxidante de L. sativa mostram que o extrato bruto e frações alteraram a produção das enzimas α-amilase, peroxidase, catalase e polifenol oxidase em, ao menos, uma das concentrações testadas, sendo um indicativo de estresse que interfere na divisão celular. Os resultados obtidos apontam para a presença de compostos com atividade inibidora ou estimulatória nas folhas de B. ungulata revelando potencial fitotóxico sobre as plântulas de alface. Palavras-chave:Bauhinia ungulata, catalase, peroxidase.ABSTRACT: Study of phytotoxic potential of extracts of Bauhinia ungulata L. on cell division and enzyme activity in lettuce seedlings. Theaim of this studywas to evaluate thephytotoxic actionofextractand fractionsobtainedfrom the leaves ofBauhiniaungulata L. inbioassaylaboratory, assessing their interferenceon cell division andactivity of the enzymesalpha-amylase, catalase, peroxidase andpolyphenoloxidaseofLactucasativa(lettu ce). The lettuce seedswere exposedto concentrationsof 250,500and 1000μg.mL -1of the extracts and fractions of Bauhinia ungulataunder controlled environmental conditions. After three days ofrootradicleprotrusion, the plants` rootswere cutand subjected tospecific preparationfor visualization andcounting of the number ofcells in eachmitotic stage. On the seventh daythe sampleswere subjected toenzymatic activityusingspecific techniquefor eachenzyme anda spectrophotometer measure. In the analysisof the mitotic indexit was observedthatthere was reduction in the number of cells in mitosis in the roots of the plants tested and thattheethyl acetate fractionsignificantly affected thecell division. The effecton the enzymesthat showed changesinthe antioxidant defensesystemof Lactucasativaindicate thatthe crude extractand the fractionsaltered theproductionofα-amylase, peroxides, catalaseand polyphenol enzymesin at least oneof the testedconcentrations, representingan indicationstress,which interferesin the celldivision.Theobtained results point out the presence ...

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Evaluation of Genotoxicity of 4-n-Nonylphenol using Vicia faba L.

Cited by 15 publications

References 4 publications

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Estudo do potencial fitotóxico de extratos de Bauhinia ungulata L. sobre a divisão celular e atividade enzimática em plântulas de alface

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Evaluation of Genotoxicity of 4-n-Nonylphenol using Vicia faba L.

Cited by 15 publications

References 4 publications

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to &lt;i&gt;Allium cepa&lt;/i&gt;

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to &lt;i&gt;Allium cepa&lt;/i&gt;

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Estudo do potencial fitotóxico de extratos de Bauhinia ungulata L. sobre a divisão celular e atividade enzimática em plântulas de alface

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Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>