Evaluation of genotoxicity and subchronic toxicity of standardized rose hip extract

Nagatomo, Akifumi; Oguri, Mikihiko; Nishida, Norihisa; Ogawa, Masahiro; Ichikawa, Atsuko; Tanaka-Azuma, Yukimasa

doi:10.1177/0960327117730881

Cited by 8 publications

(7 citation statements)

References 42 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, a rose hip extract of the Rosa canina, standardised for tiliroside concentration, was evaluated following a 90-day repeated oral dose toxicity study at 100-1,000 mg/kg in male and female rats. The results showed no abnormal symptoms or mortality in the treated animals (Nagatomo et al, 2017). The phytoconstituent dose at 50 mg/kg, being 100-fold less than 5,000 mg/kg of the methanolic leaf extract for AOT study may not be reliable to determine the safety margin between the pharmacological dose and the toxic dose.…”

Section: Discussionmentioning

confidence: 84%

“…Similarly, a rose hip extract of the Rosa canina , standardised for tiliroside concentration, was evaluated following a 90‐day repeated oral dose toxicity study at 100–1,000 mg/kg in male and female rats. The results showed no abnormal symptoms or mortality in the treated animals (Nagatomo et al, ).…”

Section: Discussionmentioning

confidence: 93%

“…A 50 mg/kg pharmacological dose of betulinic acid was 20‐fold lower than the 1,000 mg AOT dose reported to be nontoxic (Jain et al, ). Hitherto, no AOT study for koetjapic acid has been documented for comparison at 50 mg/kg, kaempferol was 200‐fold less than the 10,000 mg/kg dose reported (Shih et al, ), vitexin was 100‐fold less than the 5,000 mg/kg tested (Farsi et al, ), and tiliroside was 20‐fold less than the 1,000 mg/kg tested (Nagatomo et al, ) for AOT studies where the doses tested were nontoxic. The above evidences therefore confirmed the safety of the doses for pharmacological studies of the isolated phytoconstituents in D .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The phytochemical and anti‐inflammatory studies of Dillenia suffruticosa leaves

Abubakar

Al-Mansoub

Murugaiyah

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

The Dillenia suffruticosa leaves (Dilleniaceae), a folk medicine recommended in Southeast Asia for treating inflammation, were phytochemically studied for the first time and assessed for suppression of λ‐carrageenan‐induced paw oedema in rats. The crude methanolic extract orally administered at 5,000 mg/kg, displayed no toxicity and at 250 to 1,000 mg/kg significantly suppressed the paw oedema. Two‐isolated triterpenoids, betulinic acid (1) and koetjapic acid (2) orally administered at 50 mg/kg, significantly reduced the paw oedema, (p < 0.001) and (p < 0.005) at the fourth h onwards to 47.36% ± 2.23 and 53.43% ± 7.09, respectively, from 95.90% ± 6.88 oedema induced by λ‐carrageenan alone. 1 and the isolated flavonoids of vitexin (3), tiliroside (4), and kaempferol (5), displayed moderately more of cyclooxygenase (COX)‐2 than COX‐1 enzyme inhibition, whereas 2 was slightly more inhibition of COX‐1. The in silico molecular docking studies provided support to the in vitro COX studies that the isolated compounds formed H‐bonding with the amino acid residues at the COX‐2 catalytic sites. The triterpenoids were bound to the peroxidase, possibly inhibiting the peroxidase reaction, whereas the flavonoids interacted more at the cyclooxygenase, resembling celecoxib, therefore providing evidences that these compounds were responsible for the anti‐inflammatory properties of D. suffruticosa.

show abstract

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The phytochemical and anti‐inflammatory studies of Dillenia suffruticosa leaves

Abubakar

Al-Mansoub

Murugaiyah

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…Finally hematological tests after 90 days of toxicity dose were the same as to controlled group and there is no significant alteration in biochemistry between control and treated groups . (18) The rats that were used as experimental animals are 2-3% of body weight (4-5 gram) that is mean the weight of the liver was considered as 2.51 % of body weight in rabbits. [19] .…”

Section: Measurement the Serum Biochemical Tests For Rats In Acute To...mentioning

confidence: 99%

Median lethal Dose and Acute Toxicity of Rosa canina L: In-Vivo Study

Jasim¹,

Jasim²,

Abbas³

2022

AJPS

View full text Add to dashboard Cite

Rosa canina belongs to rosacea family. Rosa. canina has a high concentration of phyto-constituents such as flavonoids, carotenoids, triterpene and vitamins as vitamin C, E, and A Rosa canina have an anti-inflammatory and antioxidant effects. The antioxidant effect belongs to presence of large quantities of phytochemicals such as flavonoids and polyphenols. Rosa canina has been used for arthritis gout, osteoarthritis, urinary tract disorder, diabetes, inflammation and cancer. After grinding the leaves of Rosa canina, they are extracted by using ethanol solvent (cold extraction method), then the extract concentrated by rotary evaporator at 40 c° and leaving it to dry. Median lethal dose (LD50) has been examined on 84 mice (male and female) divided into seven groups, each one contains 12 (6 male and 6 female). The animals were monitored for signs and any behavior changes after administration of Rosa canina ethanol extract. Acute toxicity study was done on 20 rats (male and female for fourteen days. The weight of animals was taken at day 0, 7, and 14. At day fourteen, relative organ weight as well as histopathological examination for (heart, liver, spleen, kidney, lung, abdominal stomach, testes and ovaries) were taken. In addition to the serum biochemical tests for) blood glucose, urea, creatinine, ALT, AST and total bilirubin (were done at day fourteen. The result of this study, indicated that lethal dose 50 was 16.527 gram/kilogram. Acute toxicity study, revealed that there is no significant difference between relative organ weight of controlled and treated groups for all organs that were selected and mentioned above. In addition, there is no significant difference between serum biochemical tests for both controlled and treated groups. Finally, no changes have been found between controlled and treated groups regarding histopathology examination due to the p value was P ˃ 0.05. Conclusion According to the presented study, ethanol extract of Rosa canina showed wide range of safety depending on the result of lethal dose 50h (16.527 g/kg). Therefore, the extract considered nontoxic. No cytotoxic effect appeared by using ethanol extract of Rosa canina in acute toxicity study. This belongs to the results obtained, which include no significant difference between control and treated male and female rats in biochemistry tests and histopathological examination.

show abstract

“…The chemicals in Rosa foetida are similar to the ones found in a typical rose. According to clinical studies and evidences presented by the Australian Gene Technology Regulator, roses and their products were reported to be non-poisonous in all forms [ 21 , 22 ]. Traditional treatments also utilize red roses to treat chest pain, stomachache, blood flow, and digestive disorders [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

The effects of Rosa foetida extract along with self-care education on primary dysmenorrhea: study protocol for a randomized clinical trial

Shabani

Omidvar

Kaboudi

et al. 2022

Trials

View full text Add to dashboard Cite

Background Dysmenorrhea is one of the most common disorders among young women. Medicinal herbs are one of the alternative methods for the treatment of dysmenorrhea. This study will investigate the effect of Rosa foetida extract, along with self-care behavior education on primary dysmenorrhea among female students of Babol University of medical sciences. Methods/design A randomized clinical trial will be performed on single students, aged 18 to 24 years. The research samples will be divided into three groups. The students will receive self-care behavior education on dysmenorrhea. Following the education, two of the groups will receive Rosa foetida extract capsules and placebo capsules in two consecutive cycles every 8 h for two successive days, respectively. The capsules will have similar physical appearance. The third group will not receive any medication. Data will be collected through demographic characteristic questionnaire, visual analog scale, dysmenorrhea self-care behaviors scale questionnaire, pictorial chart, and menstrual distress scale questionnaire. In order to determine and compare the effect of pharmacological and educational interventions on the severity of dysmenorrhea in groups, an ANOVA analysis of variance test with repeated measures will be used by SPSS software version 22. Discussion The results will show the effects of Rosa foetida extract along with self-care behavior education on primary dysmenorrhea, and beneficial effects that may be found in the trial of this plant may be of use for women with the same problem. Ethics and dissemination The study is approved by the Ethics Committee of Babol University of Medical Sciences (IR.MUBABOL.REC.1397.059). Trial registration IRCT 20190318043086N1. Registered on 14 June 2019.

show abstract

Evaluation of genotoxicity and subchronic toxicity of standardized rose hip extract

Cited by 8 publications

References 42 publications

The phytochemical and anti‐inflammatory studies of Dillenia suffruticosa leaves

The phytochemical and anti‐inflammatory studies of Dillenia suffruticosa leaves

Median lethal Dose and Acute Toxicity of Rosa canina L: In-Vivo Study

The effects of Rosa foetida extract along with self-care education on primary dysmenorrhea: study protocol for a randomized clinical trial

Contact Info

Product

Resources

About