Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. &amp; Schult. F.) Backer ex K. Heyne using DNA-based markers

Singh, Samunder; Dalal, Sunita; Singh, Rohtas; Dhawan, A. K.; Kalia, Rajwant K.

doi:10.1007/s11738-012-1084-x

Cited by 63 publications

(42 citation statements)

References 25 publications

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“…These are long-lived, woody, evergreen, members of subfamily Bambusoideae of family Poaceae (Singh et al, 2012a). Most of the bamboos are shrub like, medium or dwarf with a few exceptions as a climber (Liese, 1987).…”

Section: Introductionmentioning

confidence: 99%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

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Section: Introductionmentioning

confidence: 99%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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“…Somaclonal variation induced by culture conditions limits the use of in vitro micropropagation, as it leads to changes in nuclear and cytoplasmic genomes and causes genetic differentiation of regenerated plants (D'Amato 1991; Larkin and Scowcroft 1981; Lee and Phillips 1988;Neelakandan and Wang 2012;Rodriguez-Enriquez et al 2011;Vazquez 2001;Wang and Wang 2012). Confirmation of the genetic identity with maternal material, and the genome size and reproductive normality of regenerated plants, is required before reintroduction (Nybom et al 2014;Ochatt et al 2011;Singh et al 2013;Thiem et al 2013;Thiem and Sliwinska 2003). Molecular markers are valuable tools for detecting the sequence variation of closely related genomes such as those between source plants and somaclones regenerated through tissue culture.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular markers are valuable tools for detecting the sequence variation of closely related genomes such as those between source plants and somaclones regenerated through tissue culture. Molecular marker systems-random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP)-each having its advantages and limitations, are used to estimate somaclonal variation in tissue culture (Bairu et al 2011;Nybom et al 2014;Rewers et al 2012;Singh et al 2013). Flow cytometry for somaclone genome size assessment can be used to complement molecular marker analyses (Bairu et al 2011;Ochatt et al 2011;Rewers et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis

Ślązak

Śliwińska

Saługa

et al. 2014

Plant Cell Tiss Organ Cult

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Viola uliginosa Besser is a European violet having its main distribution range in the Baltic Sea region. Today it is considered endangered and threatened. Species of Violaceae from different genera and sections are known to produce cyclotides, cyclic polypeptides of much interest due to their medicinal properties and chemical structure. The present study introduced a rare species of violet (V. uliginosa) to in vitro culture for biodiversity protection and as a model for cyclotide biosynthesis research in the Violaceae. Leaf and petiole fragments were cultured on MS medium solidified with agar and supplemented with different concentrations of plant growth regulators: TDZ, KIN and 2,4-D. Direct and indirect (via callus) organogenesis was induced on MS supplemented with TDZ (0.5 or 1 mg l -1 ) or with equal concentrations (2 mg l -1 ) of KIN and 2,4-D, followed by callus transfer on 1 mg l -1 TDZ. Shoots were rooted on MS with 2 % sucrose and 0.5 mg l -1 IBA and acclimatized. AFLP marker polymorphism was low but flow cytometry revealed that a large share of the obtained regenerants were tetraploid (2C = 4x = 2.7-2.8 pg), unlike the maternal diploid plants (2C = 2x = 1.4 pg). Eleven different cyclotides were distinguished in the aerial parts of maternal plants. Cyclotide production was significantly higher in tetraploid than in diploid plants regenerated in vitro.

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“…In past, morphological descriptions, physiological traits, cytological studies, isozymes (Gupta & Varshney 1999, Devarumath et al 2002, Agnihotri et al 2009, Singh et al 2012a, 2012b and many other techniques have been deployed to assess the genetic purity of clonally mass propagated plants. However, expression of the morphological and physiological traits may changes in response to the prevalent environmental conditions (Singh et al 2013). Due to cytological aberrations, there are always possibility of phenotypically homogeneous looking plants may behave differently during flowering/fruiting and later stages, making conclusion about genetic purity invalid.…”

Section: Introductionmentioning

confidence: 99%

“…The inherent characteristics of DNA markers such as abundantness and insensitivity to environmental conditions makes them more useful than morphological and physiological traits in establishing the identity of particular tree/clone or testing the genetic purity or tracing its genetic relationship. Earlier, random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR), amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), sequence characterized amplified region (SCAR), DNA amplification fingerprinting (DAF), Arbitrarily primed polymerase chain reaction (AP-PCR) have been successfully employed for fidelity testing in various plant species (Rani & Raina 2000, Archana et al 2013, Singh et al 2013, Mohammad et al 2016. Among these, ISSR is a very simple, quick, cost-effective, highly discriminative and reliable method that combines most of the advantages of SSRs and AFLP with the universality of RAPD (Reddy et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Testing of genetic homogeneity of elite eucalyptus clones using DNA marker

Mohammad¹,

Dahayat²,

Pardhi³

et al. 2017

Trop. Plant Res.

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Under 'Hariyali Prasar Yojna' Chhattisgarh State Forest Department has procured and planted elite eucalyptus clones on large scale in different forest circles/divisions of the state during the monsoon of 2015-16 and 2016-17. To assess the genetic homogeneity of the supplied clones, genetic fidelity testing of the procured clones was carried out using ISSR marker. The monomorphic pattern of ISSR profiles observed for the ramets of the respective clones in comparison with their mother plant confirmed the genetic purity. This also demonstrates the application of molecular marker technology for quality control in social forestry plantation.

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Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers

Cited by 63 publications

References 25 publications

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis

Testing of genetic homogeneity of elite eucalyptus clones using DNA marker

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