Evaluation of genetic diversity in a Camelina sativa (L.) Crantz collection using microsatellite markers and biochemical traits

Manca, Antonella; Pecchia, Paola; Mapelli, S.; Masella, Piernicola; Galasso, Incoronata

doi:10.1007/s10722-012-9913-8

Cited by 41 publications

(35 citation statements)

References 34 publications

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“…Two collections in Germany and Canada are available with several hundred accessions (http:// gbis.ipk-gatersleben.de/, http://pgrc3.agr.gc.ca/index_e.html). More recently, genetic maps based on RAPDs, SSRs AFLPs and SNPs have been created (Vollmann et al, 2005;Gehringer et al, 2006;Manca et al, 2013;Singh et al, 2015), and various transcriptome sequencing results have also been reported (Liang et al, 2013;Nguyen et al, 2013;Mudalkar et al, 2014;Singh et al, 2015). These efforts, as well as the draft complete genome sequence will be of great use in breeding improved camelina cultivars (Kagale et al, 2014).…”

Section: Camelina Is More Than Its Oilmentioning

confidence: 99%

Camelina, a Swiss knife for plant lipid biotechnology

Faure

Tepfer

2016

OCL

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-Camelina has emerged in the last decade as a multipurpose crop plant particularly suitable for engineering new lipids for diverse uses, including feed, biofuel and green chemistry. The rebirth of this ancient crop was based on several intrinsic favorable characteristics: robust agronomic qualities, attractive oil profile, genetic proximity with the model plant arabidopsis, ease of genetic transformation by floral dip. The need to increase both the production and diversity of plant oils, while improving the sustainability of agricultural systems, has been the driving forces behind the ever-increasing investment in camelina research. Worldwide interest in engineering camelina has led to the development of a remarkable pipeline that allows the rapid production and phenotyping of new lines; it includes specific tools, such as databases, collections of natural accessions, methods of genetic transformation and lipid analysis. Implementation of numerous metabolic pathways in camelina for the production of novel lipids has highlighted the potential as well as the versatility of this new "old" oilseed crop that is well on the way to becoming an ideal plant chassis for lipid synthetic biology.Keywords: Lipid metabolism / oilseed / omega-3 / biofuels Résumé -La cameline, véritable couteau-suisse pour la biotechnologie végétale des lipides. La caméline a émergé durant la dernière décennie comme une espèce agronomique polyvalente particulièrement adaptée à l'ingé-nierie des lipides pour divers usages comme les biocarburants, l'alimentation ou la chimie verte. La renaissance de cette espèce cultivée ancienne est fondée sur plusieurs caractéristiques : robustesse agronomique, profil en huile attractif, proximité génétique avec la plante modèle arabidopsis et très grande facilité de transformation génétique par infiltration des fleurs. Le besoin d'augmenter la production d'huile végétale tout en diversifiant la nature des lipides produits, associé à l'amélioration de la durabilité des systèmes de production, ont été les déterminants qui ont poussé le développement de la recherche autour de la caméline. L'intérêt de par le monde pour l'ingénierie des lipides chez la caméline a ainsi permis la mise en place d'un ensemble d'outils incluant des bases de données, des collections d'accessions naturelles, du matériel spécifique pour la transformation et le phénotypage, ainsi que des méthodes pour la production et la caractérisation de nouvelles variétés ou de lignées. Les nombreux exemples de mise en place de nouvelles voies métaboliques chez la caméline pour la production de lipides spécifiques illustrent le potentiel important de cette nouvelle « ancienne » espèce oleogineuse qui est en passe de devenir un chassis végétal idéal pour la biologie synthétique des lipides. Mots clés :Métabolisme lipidique / oléagineux / oméga-3 / biocarburants

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Section: Camelina Is More Than Its Oilmentioning

confidence: 99%

Camelina, a Swiss knife for plant lipid biotechnology

Faure

Tepfer

2016

OCL

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“…Because Camelina sativa is self-compatible and exhibits high levels of spontaneous self-pollination (Manca et al 2013), hybrid breeding has not been used in genetic improvement, though in theory, the introduction of male-sterility or self-incompatibility through genetic transformation could facilitate commercial hybrid production. Hybrid breeding is often used to improve crops.…”

mentioning

confidence: 99%

“…Because of its high level of self-pollination, accessions of Camelina sativa can be considered to be equivalent to near inbred lines (Manca et al 2013). While some researchers have suggested that much of the genetic load arising from deleterious recessive alleles should be purged in plants that undergo natural self-pollination, theoretical analyses of inbreeding and purging have shown that when many deleterious mutations are partly expressed in the heterozygous state and their individual effects are weak, purging is inefficient (Charlesworth et al 1990).…”

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confidence: 99%

“…To obtain the microsatellite data, we selected a single young leaf from each of the 20 tested trios of parents and a single putative hybrid offspring, extracted DNA from the leaf of each plant using the DNeasy Qiagen ™ mini-prep kit, and conducted PCR amplification of several published microsatellite loci as described by Manca et al (2013). The microsatellite loci used to verify the crosses included the loci P4E6, P7D4, P7C2, and P4C11 (Manca et al 2013).…”

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confidence: 99%

“…The microsatellite loci used to verify the crosses included the loci P4E6, P7D4, P7C2, and P4C11 (Manca et al 2013). The microsatellite genotypes of the progeny were checked against those of its two presumed parents.…”

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Testing for heterosis in traits associated with seed yield inCamelina sativa

Zelt¹,

Schoen²

2016

Can. J. Plant Sci.

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Camelina sativa is an oilseed crop that is increasingly used as a source of biopolymer and biofuel. Thirteen accessions were crossed in all pairwise combinations. Some crosses yielded progeny that exhibited best-parent heterosis for traits associated with seed yield, though mid-parent heterosis was more commonly found.Key words: Camelina, false flax, best-parent heterosis, mid-parent heterosis.Résumé : Camelina sativa est un oléagineux dont on se sert de plus en plus comme source de polymères organiques et de biocarburant. Treize obtentions ont été croisées par paires. Certains croisements ont engendré une progéniture qui exprimait la plus forte vigueur hybride pour des caractères associés au rendement grainier, bien que l'hétérosis moyen soit beaucoup plus courant. [Traduit par la Rédaction] Mots-clés : caméline, lin bâtard, hétérosis optimal, hétérosis moyen.

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Camelina as a sustainable oilseed crop: Contributions of plant breeding and genetic engineering

Vollmann

Eynck²

2015

Biotechnology Journal

140

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Camelina is an underutilized Brassicaceae oilseed plant with a considerable agronomic potential for biofuel and vegetable oil production in temperate regions. In contrast to most Brassicaceae, camelina is resistant to alternaria black spot and other diseases and pests. Sequencing of the camelina genome revealed an undifferentiated allohexaploid genome with a comparatively large number of genes and low percentage of repetitive DNA. As there is a close relationship between camelina and the genetic model plant Arabidopsis, this review aims at exploring the potential of translating basic Arabidopsis results into a camelina oilseed crop for food and non-food applications. Recently, Arabidopsis genes for drought resistance or increased photosynthesis and overall productivity have successfully been expressed in camelina. In addition, gene constructs affecting lipid metabolism pathways have been engineered into camelina for synthesizing either long-chain polyunsaturated fatty acids, hydroxy fatty acids or high-oleic oils in particular camelina strains, which is of great interest in human food, industrial or biofuel applications, respectively. These results confirm the potential of camelina to serve as a biotechnology platform in biorefinery applications thus justifying further investment in breeding and genetic research for combining agronomic potential, unique oil quality features and biosafety into an agricultural production system.

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Evaluation of genetic diversity in a Camelina sativa (L.) Crantz collection using microsatellite markers and biochemical traits

Cited by 41 publications

References 34 publications

Camelina, a Swiss knife for plant lipid biotechnology

Camelina, a Swiss knife for plant lipid biotechnology

Testing for heterosis in traits associated with seed yield inCamelina sativa

Camelina as a sustainable oilseed crop: Contributions of plant breeding and genetic engineering

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