Evaluation of fungicidal activity of extracellular filtrates of cyanobacteria – possible role of hydrolytic enzymes

Prasanna, Radha; Nain, Lata; Tripathi, Ravindramani; Gupta, Vishal; Chaudhary, Vidhi; Middha, Sheetal; Joshi, Meenakshi; Ancha, Radhika; Kaushik, B. D.

doi:10.1002/jobm.200700199

Cited by 126 publications

(76 citation statements)

References 29 publications

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“…On the other hand, the effect of the standard antibiotics on Candida albicans was variable and was more or less similar to that of the investigated algal extracts. Literature reveals that similar reports were also observed by Prashanthkumar et al (2006), Prasanna et al (2008), Shimna (2012), Sankarrao (2013) and Mugilan (2014). Thus it can be concluded that some of the extracts obtained using various solvents used in this study had higher antibacterial and antifungal activities and they are more effective when compared with some contemporary antibiotics and fungicides.…”

Section: Resultssupporting

confidence: 77%

“…Results of the experiments done by Prashanthkumar et al (2006), Prasanna et al (2008), Vijayakumar et al (2011aVijayakumar et al ( , 2011b) also showed similar results. These observations are in line with those of the present study.…”

Section: Resultssupporting

confidence: 77%

“…Literature reveals that many workers (Noaman et al, 2004;Mayer and Hamann, 2005;Gul and Hamann, 2005;Prashanthkumar et al, 2006;Prasanna et al, 2008;Vijayakumar et al, 2011aVijayakumar et al, , 2011b showed ethanol and acetone to be a good solvent for extracting antibacterial and antifungal agents from Oscillatoria latevirens, Phormidium corium, and Lynbhya martensiana. However, the present study indicates that chloroform can also be used as a solvent for extracting the antibacterial and antifungal agents.…”

Section: Resultsmentioning

confidence: 99%

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Antimicrobial Activity of Microalgae Isolated from Fresh Water Pond, Tamil Nadu, India

Mugilan¹,

Sivakami²

2016

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 77%

Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial Activity of Microalgae Isolated from Fresh Water Pond, Tamil Nadu, India

Mugilan¹,

Sivakami²

2016

Int.J.Curr.Microbiol.App.Sci

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“…Our previous investigation revealed the activities of hydrolytic enzymes in several Anabaena strains and correlation of these activities with antifungal activity (31). In the present study, we characterized a putative antifungal gene (cho) encoding a chitosanase belonging to the glycosyl hydrolase 3-like family, and its regulation was studied under different environmental conditions.…”

mentioning

confidence: 94%

Identification, Characterization, and Regulation of a Novel Antifungal Chitosanase Gene ( cho ) in Anabaena spp

Gupta

Prasanna

Natarajan

et al. 2010

Appl Environ Microbiol

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Two contrasting cyanobacterial species (Anabaena fertilissima and Anabaena sphaerica) were selected based on differences in antifungal behavior in order to study the mechanism for production of an antifungal enzyme and the genes responsible for this production. In A. fertilissima, chitosanase and antifungal activities were increased significantly under of growth-limiting conditions (8 of light and 16 h of darkness). The lack of such activities in A. sphaerica was associated with high levels of protein that accumulated during the stationary phase (at 28 days) under the same light conditions. The gene putatively responsible for chitosanase and antifungal activities was amplified using specific primers, and sequence analysis of the amplified products (1.086 and 1.101 kb in A. sphaerica and A. fertilissima, respectively) showed that they belong to the glycoside hydrolase 3 ( Cyanobacteria comprise a heterogeneous assemblage of photosynthetic prokaryotes having extraordinary biosynthetic potential and a repertoire of diverse metabolic activities. They are an important source of novel antifungal, antibacterial, and herbicidal or weedicidal compounds, which have been implicated in allelopathic interactions in water and soil (29). A majority of these metabolites are biologically active and are products of either nonribosomal polypeptide (NRP) or mixed polyketide-NRP biosynthetic pathways. The toxins produced by cyanobacteria are greatly influenced by various physiological and environmental factors, including light, temperature, nutrients, and pH (5).The antifungal properties of cyanobacterial metabolites, most of which have not been exploited, have immense potential in agriculture for use against fungal plant pathogens. In bacteria, lytic enzymes, such as chitinases, chitosanases, proteases, and ␤-1,3-glucanases, are known to have key roles in biocontrol of various soilborne fungal pathogens. Chitin is a linear polymer of 1,4-linked N-acetyl-D-glucosamine (GlcNAc) residues, and the deacetylated derivative of chitin is chitosan. In contrast to chitin, chitosan has been found in very few organisms, but it has been found in members of the Zygomycota, such as Mucor rouxii (42), Absidia coerulea (22), and Rhizopus oryzae (8). The filamentous fungi Lentinus edodes andPleurotus sajo-caju were investigated to determine their abilities to produce chitosan (30). Chitosan and chitosan-glucan complexes have been found in the mycelia of Aspergillus niger, Humicola lutea, and Fusarium moniliforme (39). Chitosanases (EC 3.2.1.132) produced by bacteria are classified into five glycoside hydrolase (GH) families (families 5, 8, 46, 75, and 80) (6, 10, 11, 12, 13). Families 5 and 8 are composed of enzymes that are hydrolytic with glycosides, and the family 46, 75, and 80 enzymes studied so far are chitosanases. This classification of chitosanases is based on amino acid sequence similarities of the catalytic domains. Recently, the family 46 chitosanase of Amycolatopsis sp. CsO-2 responsible for antifungal activity against Rhizopus oryzae ...

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“…Apparently, higher MBC observed in 50% CF + BFBF than the 50% CF could be due to the introduced microbes and subsequent emergence of cyanobacterial and fungal communities with the application of the BFBF. Cyanobacteria and fungi have been reported to be major contributors to soil MBC (Henrot and Robertson, 1994;Lovell et al, 1995;Prasanna et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Emergence of Diverse Microbes on Application of Biofilmed Biofertilizers to a Maize Growing Soil

Buddhika¹,

Athauda

Seneviratne³

et al. 2014

Ceylon J. Sci. (Biol. Sci.)

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Diverse microbial communities in the rhizosphere perform an amazing role in plant growth and productivity. However, conventional agricultural practices such as the use of chemical fertilizer (CF) and tillage have collapsed the diversity of the microbial communities. Direct application of developed fungal-bacterial communities known as biofilmed biofertilizers (BFBFs) to the soil has been introduced recently, and observed to be multi-functional and more effective than conventional biofertilizers. However, the effect of such biofertilizers on soil microbial diversity has not been studied sufficiently world over. Therefore, the current study was carried out to investigate the effects BFBFs on bacterial, cyanobacterial and fungal species in a maize growing soil. A pot experiment was conducted under greenhouse conditions with different fertilizer treatments; 100% chemical fertilizers (CF) recommended for maize, 50% CF, 50% CF + BFBF, and no fertilizer, as the control. Microbial species richness and abundance of bacteria were evaluated after two months of plant growth. Microbial biomass carbon (MBC) was estimated by using chloroform fumigation-extraction technique. Results showed that the species richness of bacteria, fungi and cyanobacteria, and abundance of bacteria were higher in 50% CF + BFBF, compared to 50% and 100% CF. This implies that the action of BFBFs tends to break dormancy of microbial seeds in the soil, resulting in emergence of a diverse microbial community, which may support natural biocontrol of pathogens. An interesting observation was the stimulation of an additional microflora of cyanobacteria by the application of BFBF. MBC levels did not show a significant difference between any of the treatments. We recommend further testing of the BFBFs for increasing microbial diversity and ecosystem functioning and hence the sustainability of maize cultivating agroecosystems.

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Evaluation of fungicidal activity of extracellular filtrates of cyanobacteria – possible role of hydrolytic enzymes

Cited by 126 publications

References 29 publications

Antimicrobial Activity of Microalgae Isolated from Fresh Water Pond, Tamil Nadu, India

Antimicrobial Activity of Microalgae Isolated from Fresh Water Pond, Tamil Nadu, India

Identification, Characterization, and Regulation of a Novel Antifungal Chitosanase Gene ( cho ) in Anabaena spp

Emergence of Diverse Microbes on Application of Biofilmed Biofertilizers to a Maize Growing Soil

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