Evaluation of Fumonisin B1 and its metabolites absorption and toxicity on intestinal cells line Caco-2

Caloni, F.; Spotti, M.; Pompa, G.; Zucco, Flavia; Stammati, Annalaura; Angelis, De

doi:10.1016/s0041-0101(02)00125-3

Cited by 34 publications

(20 citation statements)

References 36 publications

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“…In vitro cell culture models have been used to assess the bioavailability and transport of several mycotoxins (1,26,45). In our study, we used the Caco-2 cell culture model to study AFB 1 transport and intestinal toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…AFL can be readily oxidized back to AFB 1 and has therefore been suggested as a "reservoir" for AFB 1 in vivo rather than a detoxification product (5). The reduced level of this metabolite may therefore be relevant to the toxicity caused by AFB 1 . On the contrary, AFM 1 levels in culture media increased in the presence of 1 ϫ 10 10 CFU/ml GG rather than decreased as would be expected after reduced cellular uptake of AFB 1 .…”

Section: Discussionmentioning

confidence: 99%

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Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells

Gratz

El-Nezami

et al. 2007

Appl Environ Microbiol

109

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The probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B 1 (AFB 1 ) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB 1 availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB 1 levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB 1 transport from the apical to the basolateral chamber was reduced from 11.1% ؎ 1.9% to 6.4% ؎ 2.5% (P ‫؍‬ 0.019) and to 3.3% ؎ 1.8% (P ‫؍‬ 0.002) within the first hour in monolayers coincubated with GG (1 ؋ 10 10 and 5 ؋ 10 10 CFU/ml, respectively). GG (1 ؋ 10 10 and 5 ؋ 10 10 CFU/ml) bound 40.1% ؎ 8.3% and 61.0% ؎ 6.0% of added AFB 1 after 1 h, respectively. AFB 1 caused significant reductions of 30.1% (P ‫؍‬ 0.01), 49.4% (P ‫؍‬ 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 ؋ 10 10 CFU/ml GG after 24 h protected against AFB 1 -induced reductions in transepithelial resistance at both 24 h (P ‫؍‬ 0.002) and 48 h (P ‫؍‬ 0.04). DNA fragmentation was apparent in cells treated only with AFB 1 cells but not in cells coincubated with either 1 ؋ 10 10 or 5 ؋ 10 10 CFU/ml GG. GG reduced AFB 1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells

Gratz

El-Nezami

et al. 2007

Appl Environ Microbiol

109

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“…Among the known FB 1 metabolites, aminopentol (HFB 1 ) is the totally hydrolyzed metabolite (Hopmans and Murphy, 1993), detectable in corn by-products, after processing at high temperature under alkaline conditions. HFB 1 has a toxicity comparable with FB 1 and higher absorption in vivo (Hopmans et al, 1997) and in vitro (Caloni et al, 2002) than that demonstrated for the parent compound. Culturing the intestinal cell line Caco-2 on semi-permeable insert, which separates the two different compartments (apical and basolateral side) simulates the real intestinal environment, it is thus possible to evaluate the passage and the absorption of FB 1 and HFB 1 into the cells.…”

Section: Introductionmentioning

confidence: 82%

In vitro Study With Caco-2 Cells on Fumonisin B1: Aminopentol Intestinal Passage and Role of P-Glycoprotein

et al. 2005

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“…FB 1 , pHFB 1 , pHFB 2 and HFB 1 metabolites presented poor toxicity for the human intestinal cell line Caco-2 when cells were treated with them (in the range of 1-138 mM) for 48 h (Caloni et al 2002). Although FB 1 appears the most toxic to cells, HFB 1 , losing the tricarballylic acid chain is more bioavailable than FB 1 on intestinal cells, indicating that risk evaluations of the metabolites of FBs are also necessary (Caloni et al 2002).…”

Section: The Toxicity Of Fbs and Their Metabolitesmentioning

confidence: 99%

“…Although FB 1 appears the most toxic to cells, HFB 1 , losing the tricarballylic acid chain is more bioavailable than FB 1 on intestinal cells, indicating that risk evaluations of the metabolites of FBs are also necessary (Caloni et al 2002). In another study, a significant increase in sphinganine (up to 7000 % compared to control cells) was observed with FB 1 , FB 2 , FB 3 , HFB 1 and pHFB 1 , and sphingosine levels remained almost unchanged, indicating that all substrates effectively inhibited ceramide synthase.…”

Section: The Toxicity Of Fbs and Their Metabolitesmentioning

confidence: 99%

Fumonisins: oxidative stress-mediated toxicity and metabolism in vivo and in vitro

Wang

Wan

et al. 2015

Arch Toxicol

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Fumonisins (FBs) are widespread Fusarium toxins commonly found as corn contaminants. FBs could cause a variety of diseases in animals and humans, such as hepatotoxic, nephrotoxic, hepatocarcinogenic and cytotoxic effects in mammals. To date, almost no review has addressed the toxicity of FBs in relation to oxidative stress and their metabolism. The focus of this article is primarily intended to summarize the progress in research associated with oxidative stress as a plausible mechanism for FB-induced toxicity as well as the metabolism. The present review showed that studies have been carried out over the last three decades to elucidate the production of reactive oxygen species (ROS) and oxidative stress as a result of FBs treatment and have correlated them with various types of FBs toxicity, indicating that oxidative stress plays critical roles in the toxicity of FBs. The major metabolic pathways of FBs are hydrolysis, acylation and transamination. Ceramide synthase, carboxylesterase FumD and aminotransferase FumI could degrade FB1 and FB2. The cecal microbiota of pigs and alkaline processing such as nixtamalization can also transform FB1 into metabolites. Most of the metabolites of FB1 were less toxic than FB1, except its partial (pHFB1) metabolites. Further understanding of the role of oxidative stress in FB-induced toxicity will throw new light on the use of antioxidants, scavengers of ROS, as well as on the blind spots of metabolism and the metabolizing enzymes of FBs. The present review might contribute to reveal the toxicity of FBs and help to protect against their oxidative damage.

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Evaluation of Fumonisin B1 and its metabolites absorption and toxicity on intestinal cells line Caco-2

Cited by 34 publications

References 36 publications

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells

In vitro Study With Caco-2 Cells on Fumonisin B1: Aminopentol Intestinal Passage and Role of P-Glycoprotein

Fumonisins: oxidative stress-mediated toxicity and metabolism in vivo and in vitro

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Evaluation of Fumonisin B1 and its metabolites absorption and toxicity on intestinal cells line Caco-2

Cited by 34 publications

References 36 publications

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B 1 Transport, Metabolism, and Toxicity in Caco-2 Cells

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B 1 Transport, Metabolism, and Toxicity in Caco-2 Cells

In vitro Study With Caco-2 Cells on Fumonisin B1: Aminopentol Intestinal Passage and Role of P-Glycoprotein

Fumonisins: oxidative stress-mediated toxicity and metabolism in vivo and in vitro

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Product

Resources

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Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells

Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B ₁ Transport, Metabolism, and Toxicity in Caco-2 Cells