In vitro Study With Caco-2 Cells on Fumonisin B1: Aminopentol Intestinal Passage and Role of P-Glycoprotein

Caloni, F.; Stammati, Annalaura; Raimondi, Ferdinando; Angelis, De

doi:10.1007/s11259-005-0063-8

Cited by 6 publications

(3 citation statements)

References 6 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This mycotoxin was classified as highly lipophilic, obtaining a logP value higher than the limit for bioavailability. BEA was also identified as a good substrate for Pgp, as it has been observed for other Fusarium mycotoxins and their metabolites [93], while intestinal peptide transporter 1 (PepT1) and intestinal bile acid transporter (ASBT) were not predicted to be implicated in BEA transport mechanisms. Altogether, these data support the validity of this method, opening a new possibility for toxicological modeling [78].…”

Section: Simulated Intestinal Environmentmentioning

confidence: 87%

Beauvericin and Enniatins: In Vitro Intestinal Effects

Bertero

Fossati

Tedesco

et al. 2020

Toxins

View full text Add to dashboard Cite

Food and feed contamination by emerging mycotoxins beauvericin and enniatins is a worldwide health problem and a matter of great concern nowadays, and data on their toxicological behavior are still scarce. As ingestion is the major route of exposure to mycotoxins in food and feed, the gastrointestinal tract represents the first barrier encountered by these natural contaminants and the first structure that could be affected by their potential detrimental effects. In order to perform a complete and reliable toxicological evaluation, this fundamental site cannot be disregarded. Several in vitro intestinal models able to recreate the different traits of the intestinal environment have been applied to investigate the various aspects related to the intestinal toxicity of emerging mycotoxins. This review aims to depict an overall and comprehensive representation of the in vitro intestinal effects of beauvericin and enniatins in humans from a species-specific perspective. Moreover, information on the occurrence in food and feed and notions on the regulatory aspects will be provided.

show abstract

Section: Simulated Intestinal Environmentmentioning

confidence: 87%

Beauvericin and Enniatins: In Vitro Intestinal Effects

Bertero

Fossati

Tedesco

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…In turn, the established cell monolayer can be challenged from the apical (luminal) site as well as the basolateral site with toxins as well as other antigens and allow a wide range of functional parameters to be measured (Shimizu 2010 ; Sun et al 2008 ; Sun and Pang 2007 ). In addition, this Caco-2 cell system is a commonly used model to study the rate of absorption and excretion of mycotoxins across the intestinal epithelium (Berger et al 2003 ; Caloni et al 2005 , 2006 ; Pfeiffer et al 2011 ; Schrickx et al 2006 ; Tep et al 2007 ; Videmann et al 2007 , 2009 ). Although the epithelial permeability for individual mycotoxins is beyond the scope of this review, the transepithelial transport of different mycotoxins has been briefly described and summarized in Electronic Supplementary Material, Table 1 .…”

Section: Experimental Models Used To Assess Intestinal Permeabilitymentioning

confidence: 99%

The intestinal barrier as an emerging target in the toxicological assessment of mycotoxins

et al. 2016

View full text Add to dashboard Cite

Mycotoxins, the secondary metabolites of fungal species, are the most frequently occurring natural food contaminants in human and animal diets. Risk assessment of mycotoxins focused as yet on their mutagenic, genotoxic and potential carcinogenic effects. Recently, there is an increasing awareness of the adverse effects of various mycotoxins on vulnerable structures in the intestines. In particular, an impairment of the barrier function of the epithelial lining cells and the sealing tight junction proteins has been noted, as this could result in an increased translocation of luminal antigens and pathogens and an excessive activation of the immune system. The current review aims to provide a summary of the available evidence regarding direct effects of various mycotoxins on the intestinal epithelial barrier. Available data, based on different cellular and animal studies, show that food-associated exposure to certain mycotoxins, especially trichothecenes and patulin, affects the intestinal barrier integrity and can result in an increased translocation of harmful stressors. It is therefore hypothesized that human exposure to certain mycotoxins, particularly deoxynivalenol, as the major trichothecene, may play an important role in etiology of various chronic intestinal inflammatory diseases, such as inflammatory bowel disease, and in the prevalence of food allergies, particularly in children.Electronic supplementary materialThe online version of this article (doi:10.1007/s00204-016-1794-8) contains supplementary material, which is available to authorized users.

show abstract

“…Fumonisins may exert multiorgan toxicity in farm and laboratory animals and they are suspected risk factors for esophageal and liver tumors, neural tube defects, and cardiovascular problems in highly exposed human populations. , From a structural point of view, fumonisins are related to sphingoid bases (Figure ) and they may disrupt sphingolipids rheostat leading to cell growth arrest and apoptosis . Concerning the toxicity of HFB1 and pHFB1, their effects on human and animal health are still controversial as they showed a limited distribution in food and an apparently lower toxicity in vivo (albeit the molecular mechanisms are still unclear), while keeping relevant degrees of toxicity in some models. − Interestingly, fumonisins may be also CerS substrates in vitro and in vivo , where the formation of N-acylated forms with fatty acids of various chain lengths was observed. In particular, N-acyl forms proved to be more cytotoxic and more rapidly adsorbed by cells in vitro , but neither the mechanism of action nor the role in the fumonisins group toxicity have been clarified yet. , EFSA recently established group health-based guidance values for fumonisins resulting in a total daily intake (TDI) of 1.0 μg FB1/kg bw per day .…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Fumonisin B1 Toxicity: A Computational Perspective beyond the Ceramide Synthases Inhibition

Dellafiora

Galaverna

Dall’Asta

2018

Chem. Res. Toxicol.

View full text Add to dashboard Cite

Fumonisins are mycotoxins produced by Fusarium fujikuroi species complex that may contaminate food and feed threatening human and animal health. Among the fumonisins group, fumonisin B1 is the most widespread and best characterized in terms of toxicity, while additional toxicological data on its congeners, such as N-acylated and hydrolyzed forms, need to be collected to support the group-based risk assessment. The inhibition of ceramide synthase has been identified as the key molecular mechanism of fumonisins toxicity resulting in modifications of sphingolipids rheostat. However, the existence of ancillary mechanisms and biological targets are likely to occur given the growing number of evidence reporting the multitarget mechanisms of mycotoxins toxicity. Therefore, in the framework of the early warning analysis of multitarget toxicity of fumonisins group, the present study aimed at searching potential targets for future hazard characterization studies of fumonisin B1 and its hydrolyzed and N-acetylated forms. In particular, on the basis of structural analogies with known inhibitors, the molecular interaction between N-acylated and hydrolyzed forms of fumonisin B1 and either ceramide transfer protein or sphingosine kinase I was assessed with a molecular modeling study. Our results pointed out that the molecular features of N-acylated hydrolyzed fumonisin B1 and hydrolyzed fumonisin B1 may allow the interaction with the ceramide transfer protein and with the sphingosine kinase I enzyme, respectively. Overall, our results identified such proteins as relevant targets that might take part in fumonisins group toxicity, adding plausible mechanistic insights to better understand fumonisins toxicity. Moreover, possible divergences in the mechanisms of action of fumonisin B1 and its modified forms were identified pointing out the need to assess their relevance with high priority to enhance the understanding of group toxicity.

show abstract

In vitro Study With Caco-2 Cells on Fumonisin B1: Aminopentol Intestinal Passage and Role of P-Glycoprotein

Cited by 6 publications

References 6 publications

Beauvericin and Enniatins: In Vitro Intestinal Effects

Beauvericin and Enniatins: In Vitro Intestinal Effects

The intestinal barrier as an emerging target in the toxicological assessment of mycotoxins

Mechanisms of Fumonisin B1 Toxicity: A Computational Perspective beyond the Ceramide Synthases Inhibition

Contact Info

Product

Resources

About