Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Nurcholis, Waras; Khumaida, Nurul; Syukur, Muhamad; Bintang, Maria

doi:10.20884/1.jm.2017.12.2.350

Cited by 15 publications

(17 citation statements)

References 15 publications

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“…For this reason, Trolox can be considered as the proper counterpart of Vitamin E to examine its actions in aqueous radical environments on its own and in combination with other antioxidant compounds. For 2,2-diphenyl picrylhydrazyl (DPPH) method proposed by Nurcholis et al 51 , with modification. Briefly, 100 µl O. aristatus ethanol extract was added to 100 µl of 125 µM DPPH in methanol at the 96-well microplate (Costar-USA).…”

Section: Methodsmentioning

confidence: 99%

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Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Batubara

Komariah

Sandrawati

et al. 2020

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Orthosiphon aristatus (Blume) Miq. of the Lamiaceae family, called as kumis kucing in Indonesia, is a valuable medicinal plant for their pharmacological properties. The present study comprised of fifteen genotypes of O. aristatus was undertaken to evaluate the genotypes based on phytochemical content and pharmacological activities of leaves ethanol extract. Chemometric analysis (correlation and principal component analysis) was also used to investigate the genetic variability based on phytochemical content and pharmacological activities of O. aristatus genotypes. Results of phytochemical characterization showed that total phenolic ranged from 1.48 to 36.08 (maximum in A15) mg GAE/g DW, total flavonoid ranged from 0.10 to 3.07 (maximum in A15) mg QE/g DW, sinensetin ranged from 0.36 to 4.02 (maximum in A11) mg/g DW, and rosmarinic acid ranged 0.06 to 7.25 (maximum in A7) mg/g DW. Antioxidant activity was tested using DPPH and FRAP assay. Antioxidant results showed that DPPH ranged from 1.68 to 15.55 (maximum in A15) μmol TE/g DW and FRAP ranged from 0.07 to 1.60 (maximum in A1 and A7) μmol TE/g DW. The genotype A8 showed the highest cytotoxic activities against HeLa (66.25%) and MCF-7 (61.79%) cell lines. Maximum α-glucosidase inhibitory activity was recorded in genotype A2 with the value of 62.84%. The genotypes A1, A2, A7, A11, and A15 were identified as superior based on their phytochemicals content and pharmacological activities coupled with chemometric analysis. This finding is important for breeding studies and also the pharmaceutical perspective of O. aristatus.

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Section: Methodsmentioning

confidence: 99%

“…For 2,2-diphenyl picrylhydrazyl (DPPH) method proposed by Nurcholis et al 51 , with modification. Briefly, 100 µl O. aristatus ethanol extract was added to 100 µl of 125 µM DPPH in methanol at the 96-well microplate (Costar-USA).…”

Section: Methodsmentioning

confidence: 99%

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Batubara

Komariah

Sandrawati

et al. 2020

Sci Rep

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“…Ten rhizomes of Curcuma aeruginosa accessions were obtained from result selection of previous research, samples collected from different regions in East Java, Central Java, Yogyakarta, West Java and Jambi in February 2015, based on metabolites contents and biological activities [10,[21][22][23]. Ten C. aeruginosa accessions were namely given codes with KL (Klewer), PK (Pakem), BH (Beringharjo), GK (Gunung Kidul), KP (Kulonprogo), PW (Purworejo), MD (Madura), LC (Losari Cirebon), CB (Ciampea Bogor) and MB (Muara Bungo).…”

Section: Methodsmentioning

confidence: 99%

“…The metabolism and accumulation of secondary metabolites in the medicinal plant influenced by genetic and environmental factors [20]. Previous studies [8,10,21] showed the existence of different curcuminoids contents and pharmacological activities of Curcuma aeruginosa accessions from different location of Indonesia. The information of curcuminoids contents of Curcuma aeruginosa accessions were still limited as they cultivated in the same environmental conditions.…”

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Variability of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin Contents in Ethanolic Extract from Ten Curcuma aeruginosa Roxb. Cultivated in West Java, Indonesia

et al. 2019

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Ten accessions of Curcuma aeruginosa Roxb. were grown under the same environment in Bogor, West Java, Indonesia was investigated for curcumin, demethoxycurcumin and bisdemethoxycurcumin contents using HPLC. No significant variations were observed among C. aeruginosa accessions for all the studied compounds. According to the results, curcumin content (mg/100 g extract) varied between 1.08 in accession Kulonprogo to 3.19 in accession Gunung Kidul. The accessions Muara Bungo and Kulonprogo had the highest (1.66) and lowest (0.51) levels of demethoxycurcumin (mg/100 g extract). Also, bisdemethoxycurcumin content varied from 0.05 to 0.49 mg/100 g extract in accessions Ciampea Bogor and Purworejo, respectively. Finally, curcuminoid contents (mg/100 g extract), accumulation of curcumin, demethoxycurcumin and bisdemethoxycurcumin contents, ranged from 1.64 to 5.05 in accessions Kulonprogo and Gunung Kidul, respectively. Hierarchical cluster analysis showed that 10 accessions could be divided into three groups. Group 1 composed of Gunung Kidul, Muara Bungo, Pakem and Beringharjo accessions. These accessions characterized by high of curcumin, demethoxycurcumin, and bisdemethoxycurcumin contents. Group 2 formed two accessions (Klewer and Purworejo) that showed the high only bisdemethoxycurcumin content. Accessions of Kulonprogo, Losari Cirebon, Madura and Ciampea Bogor, in group 3, characterized by low curcumin, demethoxycurcumin and bisdemethoxycurcumin contents. In this study, Gunung Kidul, Muara Bungo, Pakem and Beringharjo accessions have a high in curcuminoid content that showed a high potential for use in future plant breeding programs.

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“…C. aeruginosa contains several bioactive compounds such as flavonoid, phenolic acids (Nurcholis et al 2016b), curcumin, demethoxycurcumin and bisdemethoxycurcumin (Nurcholis et al 2019(Nurcholis et al , 2016a, germacrone (Hossain et al 2015;Srivilai et al 2018), isocurcumenol, zederone, curcumenol, dehydrocurdione, zedoarondiol (Suphrom et al 2012), tropolone, eucalyptol, and curcumol (Fitria et al 2019). The reported biological activities of C. aeruginosa and its metabolites include antimicrobial (Akarchariya et al 2017), analgesic effect (Reanmongkol et al 2006), antiandrogenic (Suphrom et al 2012), uterine relaxant effect (Thaina et al 2009), antioxidant (Nurcholis et al 2017), antinociceptive (Hossain et al 2015), hair growth promotor (Pumthong et al 2012), and anticancer (Fitria et al 2019). Therefore, it is crucial to select C. aeruginosa accessions with high phytochemical content and high pharmacological activities, which can be developed for plant breeding programs.…”

Section: Introductionmentioning

confidence: 99%

GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent

et al. 2021

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Abstract. Nurcholis W, Khumaida N, Bintang M, Syukur M. 2021. GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent. Biodiversitas 22: 1179-1186. This work aimed to evaluate the bioactive compounds and anticancer activity in rhizome extract of ten Curcuma aeruginosa accessions to explore their pharmacological values. The GC-MS analysis was used to identify bioactive compounds. The cytotoxic activity was performed against MCF-7 (Human breastadeno carcinoma) and Vero cell lines using MTT assay. The GC-MS analysis revealed 71 of the compounds as sesquiterpenes (36), monoterpenes (20), phenolics (5), diterpenes (4), phenanthrene (1), tetrapeptides (1), oxazole (1), triazine (1), piperidine (1), and oxygenated hydrocarbons (1). The isocurcumenol was the most dominant metabolite in ethanol extract of C. aeruginosa rhizome, with the highest produced by KP accession (22.01%) followed MD accession (21.12%). However, camphor and β-elemene were the metabolites produced by all accessions studied. In the Vero cell line as a normal cell, the cytotoxic activity varied from 13.28% (MD) to 45.17% (PW). Furthermore, the cytotoxic activity ranged from 1.16% (LC) to 49.70% (MD) against the MCF-7 cell line. The highest anticancer activity was produced in MD accessions; thus, it can be used as a source of quality raw materials for the pharmaceutical and food industry. Besides that, it can also be further developed to obtain superior varieties through plant breeding programs.

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Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Cited by 15 publications

References 15 publications

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

Variability of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin Contents in Ethanolic Extract from Ten Curcuma aeruginosa Roxb. Cultivated in West Java, Indonesia

GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent

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