Evaluation of Four Commercial Enzyme-Linked Immunosorbent Assays for the Diagnosis of Bovine Paratuberculosis in Chilean Dairy Herds

Abstract

Abstract. The accuracy of 4 commercial enzyme-linked immunosorbent assays (ELISAs) for diagnosis of bovine paratuberculosis was compared using sera from 53 Mycobacterium avium subsp. paratuberculosis (MAP) fecal culture-positive dairy cows (cases) and sera from 345 dairy cattle resident in 11 fecal culturenegative herds on 2 consecutive occasions 1 year apart (controls). The specificity of all 4 ELISA kits was .99%, and their diagnostic sensitivity ranged from 30.2% to 41.5%. Pairwise comparison of ELISAs found no significant differences (McNemar's chi-square test . 0.05), and assay agreement for categorical assay interpretation (positive or negative) was high (.98%) with k values ranging from 0.84 to 0.95. Receiver operating characteristic (ROC) curve analysis and the corresponding area under the ROC curves indicate that kit B had the highest overall accuracy. Thus, all 4 ELISA kits for bovine paratuberculosis had comparable accuracy when tested on Chilean dairy cattle, with kit B having a slight statistical advantage based on ROC area under the curve analysis. This suggests that any of the 4 kits could be appropriate for herd certification and for paratuberculosis control programs on Chilean dairy cattle.

MAP1272c Encodes an NlpC/P60 Protein, an Antigen Detected in Cattle with Johne's Disease

“…Collectively, both that study and the current study demonstrate that recombinant proteins tested thus far do not improve upon commercially available ELISAs. However, there are many more candidates remaining to be tested, and in all cases, they must be measured against commercially available tests, which serve as the standard that they must surpass (12).…”

Kars Yöresi Sığırlarında Subklinik Paratuberkülozun Seroprevalansı

“…Son yıllarda yüksek sensitivite ve spesifite ile özellikle seroprevalans çalışmalarında ELISA hemen hemen kullanılan tek yöntem gibi görünmektedir [4][5][6]11,19] . Yapılan çalışmalarda ELISA'nın sensitivite değeri %30.2-%51.0, spesifite değeri ise %95 ile %99 arasında değiştiği belirtilmiştir [4,5,17,19,20] . Fakat son yıllarda geliştirilen ticari kitlerde sensitivitenin %74.1'e kadar çıktığı ve spesifite oranının ise %99'un üstünde olduğu bildirilmiştir [4,17,19,20] .…”

“…Yapılan çalışmalarda ELISA'nın sensitivite değeri %30.2-%51.0, spesifite değeri ise %95 ile %99 arasında değiştiği belirtilmiştir [4,5,17,19,20] . Fakat son yıllarda geliştirilen ticari kitlerde sensitivitenin %74.1'e kadar çıktığı ve spesifite oranının ise %99'un üstünde olduğu bildirilmiştir [4,17,19,20] . Prospektüse göre, araştırmamızda kullanılan ELISA kitinin sensitivite ve spesifite oranları sırasıyla %64.7 ve %99.2 olarak belirtilmiştir.…”

Search forMycobacterium aviumSubspeciesparatuberculosisAntigens for the Diagnosis of Paratuberculosis

“…Among tests to detect serum antibody to MAP, ELISAs are the most widely used. Several commercial ELISA kits for bovine PTB are currently available, and multiple studies have compared their accuracy [9, 10]. Comparative studies of ELISAs with different antigens have shown discrepancies in the ability of these tests to identify all infected animals [11].…”