Evaluation of fouling mechanisms in asymmetric microfiltration membranes using advanced imaging

Marroquin, Milagro; Vu, Anh Tuan; Bruce, Terri F.; Wickramasinghe, S. Ranil; Zhao, Lixin; Husson, Scott M.

doi:10.1016/j.memsci.2014.03.077

Cited by 12 publications

(5 citation statements)

References 34 publications

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“…The areal mass of BSA on the membrane surfaces at different filtration times was determined by applying the calibration curve to the intensity profile. Previous studies have combined flux decline measurements with quantitative visual analysis of CLSM images to better understand protein fouling [ 45 ]. Here, we used this combined method to study the location and extent of BSA fouling on membranes patterned with a herringbone geometry.…”

Section: Resultsmentioning

confidence: 99%

Evaluating Protein Fouling on Membranes Patterned by Woven Mesh Fabrics

Malakian

Husson

2021

Membranes

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Membrane surface patterning is one approach used to mitigate fouling. This study used a combination of flux decline measurements and visualization experiments to evaluate the effectiveness of a microscale herringbone pattern for reducing protein fouling on polyvinylidene fluoride (PVDF) ultrafiltration membranes. Thermal embossing with woven mesh stamps was used for the first time to pattern membranes. Embossing process parameters were studied to identify conditions replicating the mesh patterns with high fidelity and to determine their effect on membrane permeability. Permeability increased or remained constant when patterning at low pressure (≤4.4 MPa) as a result of increased effective surface area; whereas permeability decreased at higher pressures due to surface pore-sealing of the membrane active layer upon compression. Flux decline measurements with dilute protein solutions showed monotonic decreases over time, with lower rates for patterned membranes than as-received membranes. These data were analyzed by the Hermia model to follow the transient nature of fouling. Confocal laser scanning microscopy (CLSM) provided complementary, quantitative, spatiotemporal information about protein deposition on as-received and patterned membrane surfaces. CLSM provided a greater level of detail for the early (pre-monolayer) stage of fouling than could be deduced from flux decline measurements. Images show that the protein immediately started to accumulate rapidly on the membranes, likely due to favorable hydrophobic interactions between the PVDF and protein, followed by decreasing rates of fouling with time as protein accumulated on the membrane surface. The knowledge generated in this study can be used to design membranes that inhibit fouling or otherwise direct foulants to deposit selectively in regions that minimize loss of flux.

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Section: Resultsmentioning

confidence: 99%

Evaluating Protein Fouling on Membranes Patterned by Woven Mesh Fabrics

Malakian

Husson

2021

Membranes

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“…In addition to that, CLSM's data analysis enables volume view of stacks of images acquired individually in a wide range of wavelengths, including the 488/521 nm excitation/emission wavelengths applied for labeling of IgG antibodies, the molecule of interest in this research. This non‐invasive optical microscopy technique has been previously reported for characterization of membrane fouling by proteins, polyphenols and polysaccharides 22–24 . The combined approach reported here enables measurement of local, intramodule TMP and determination of protein layer thicknesses, providing insights on protein accumulation at membrane surfaces during TFF.…”

Section: Introductionmentioning

confidence: 90%

“…reported for characterization of membrane fouling by proteins, polyphenols and polysaccharides. [22][23][24] The combined approach reported here enables measurement of local, intramodule TMP and determination of protein layer thicknesses, providing insights on protein accumulation at membrane surfaces during TFF. the clean used filters were reutilized, or else, the pressure sensors were replaced in order to test whether the same were defective, and when that was not the case, the cassette filters were replaced by new ones.…”

Section: This Non-invasive Optical Microscopy Technique Has Been Prev...mentioning

confidence: 99%

Intramodule pressure profiles and protein accumulation during tangential flow filtration

Cunha,

Zuponcic,

Rossi

et al. 2023

Biotechnology Progress

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Tangential flow filtration (TFF) through a 30 kDa nominal molecular weight cut‐off (MWCO) ultrafiltration membrane is widely employed to concentrate purified monoclonal antibodies (mAbs) to levels required for their formulation into injectable biologics. While TFF has been used to remove casein from milk for cheese production for over 35 years, and in pharmaceutical manufacture of biotherapeutic proteins for 20 years, the rapid decline in filtration rate (i.e., flux) at high protein concentrations is a limitation that still needs to be addressed. This is particularly important for mAbs, many of which are 140–160 kDa immunoglobulin G (IgG) type proteins recovered at concentrations of 200 mg/mL or higher. This work reports the direct measurement of local transmembrane pressure drops and off‐line confocal imaging of protein accumulation in stagnant regions on the surface of a 30 kDa regenerated cellulose membrane in a flat‐sheet configuration widely used in manufacture of biotherapeutic proteins. These first‐of‐a‐kind measurements using 150 kDa bovine IgG show that while axial pressure decreases by 58 psi across a process membrane cassette, the decrease in transmembrane pressure drop is constant at about 1.2 psi/cm along the 20.7 cm length of the membrane. Confocal laser scanning microscopy of the membrane surface at the completion of runs where retentate protein concentration exceeds 200 mg/mL, shows a 50 μm thick protein layer is uniformly deposited. The localized measurements made possible by the modified membrane system confirm the role of protein deposition on limiting ultrafiltration rate and indicate possible targets for improving membrane performance.

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“…The radius of curvature, net aperture, material, film layer and other parameters of each lens are initially adjusted based on the knowledge of imaging optics. The preliminary design is eventually optimized using the Zemax optimization function, with common operands such as LONA, SPHA (optimized spherical aberration), all the way up to the level envisioned in this paper [3] .…”

Section: Objective Lens Designmentioning

confidence: 99%

Design of optical path and mechanical structure for non-contact confocal scanning imaging system

liu,

Liao,

Liu

et al. 2024

International Conference on Optics and Machine Vision (ICOMV 2024)

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This paper designs a non-contact laser confocal system and its mechanical structure. A relatively simple optical structure was sought to be used while ensuring that the design specifications were accomplished. The article designed the lens set and analyzed its imaging quality using Zemax software. The results show that the focusing dispersion spot diameter of the microfocusing system is less than 3 μm, and the RMS radius is less than 1 μm; the focusing dispersion spot diameter of the detection and acquisition system is less than 15 μm, and the RMS radius is less than 5 μm; and the MTF curves of all subsystems are close to the diffraction limit, which indicates that the optical transmission quality is good. In addition, the mechanical structure of the optical path system is designed in this paper to ensure the compactness and simplicity of the whole system.Finally, it can be seen from the physical experiment and software simulation that the designed confocal optical path system has good optical imaging performance

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Evaluation of fouling mechanisms in asymmetric microfiltration membranes using advanced imaging

Cited by 12 publications

References 34 publications

Evaluating Protein Fouling on Membranes Patterned by Woven Mesh Fabrics

Evaluating Protein Fouling on Membranes Patterned by Woven Mesh Fabrics

Intramodule pressure profiles and protein accumulation during tangential flow filtration

Design of optical path and mechanical structure for non-contact confocal scanning imaging system

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