Evaluation of fibrin-gelatin hydrogel as biopaper for application in skin bioprinting: An in-vitro study

Hakam, Mohammad Sadjad; Imani, Rana; Abolfathi, Nabiollah; Fakhrzadeh, Hossein; Sharifi, Ali Mohammad

doi:10.3233/bme-161617

Cited by 27 publications

(18 citation statements)

References 19 publications

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“…Similarly, the use of fibrin microspheres seeded with stem cells also provides an opportunity for future investigation. Finally, hybrid bioinks containing fibrin have shown promise for use in 3D printing functional tissues from stem cells [85].…”

Section: Fibrinmentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

111

113

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Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues.

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Section: Fibrinmentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

111

113

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“…For example, alginate has been previously blended with gelatin as alginate gelates easily in the presence of Ca 2+ and has a good mechanical strength, and gelatin offers RGD sequences for cell adhesion and migration [ 29 ]. Biomaterials that are commonly blended also include collagen blended with nanofibrous [ 80 ], alginate blended with honey [ 149 ], gelatin blended with fibrin [ 104 ] and methacrylate gelatin blended with polyethylene glycol [ 158 ]. Crosslinking of the blended bioink, through thermal, chemical and photo-crosslinking, is critical for the maintenance of the 3D structure that provides the proper mechanical properties and microenvironment for cellular activities [ 166 ].…”

Section: Discussion and Future Perspectivementioning

confidence: 99%

“…Therefore, as an initial hydrogel for wound closure, fibrin plays major roles in reducing wound contraction and supporting wound repair. A fibrin-gelatin blended hydrogel has been reported to be used as a bio-paper for skin bioprinting, showing that it provides a natural scaffold for fibroblast embedding and culturing [ 104 ].…”

Section: Bioink Componentsmentioning

confidence: 99%

“…A newly developed bioink composed of gelatin, alginate, fibrinogen and fibroblasts has been reported to closely model the structure of human skin at the macroscopic and molecular level [ 152 ] ( Figure 6 A,B). Similarly, a fibrin-gelatin hybrid bioink has also been prepared to fabricate a hydrogel with inoculated fibroblasts for skin bioprinting [ 104 ]. Furthermore, 3D skin structures have also been printed using a collagen/gelatin hydrogel, where the gelatin pattern was printed into the collagen groove under 20 °C, and the gelatin was selectively liquefied and removed under 40 °C in an incubator to form a fluid channel scaffold ( Figure 6 C) [ 153 ].…”

Section: Current Bioink Products For Skin Bioprintingmentioning

confidence: 99%

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Advances in the Research of Bioinks Based on Natural Collagen, Polysaccharide and Their Derivatives for Skin 3D Bioprinting

Zheng

et al. 2020

Polymers

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The skin plays an important role in protecting the human body, and wound healing must be set in motion immediately following injury or trauma to restore the normal structure and function of skin. The extracellular matrix component of the skin mainly consists of collagen, glycosaminoglycan (GAG), elastin and hyaluronic acid (HA). Recently, natural collagen, polysaccharide and their derivatives such as collagen, gelatin, alginate, chitosan and pectin have been selected as the matrix materials of bioink to construct a functional artificial skin due to their biocompatible and biodegradable properties by 3D bioprinting, which is a revolutionary technology with the potential to transform both research and medical therapeutics. In this review, we outline the current skin bioprinting technologies and the bioink components for skin bioprinting. We also summarize the bioink products practiced in research recently and current challenges to guide future research to develop in a promising direction. While there are challenges regarding currently available skin bioprinting, addressing these issues will facilitate the rapid advancement of 3D skin bioprinting and its ability to mimic the native anatomy and physiology of skin and surrounding tissues in the future.

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“…Though progressive loss of the uncrosslinked gelatin molecules in the long-term in vitro cultured gelatin/alginate/fibrin constructs has been detected, the living 3D constructs can be maintained properly over four weeks until the new tissue/organ generation [11,12,13]. The representative literature on the gelatin-based hydrogels as ‘bioinks’ in tissue/organ 3D bioprinting is summarized in Table 3 except those appeared in Table 2 [97,98,99,100,101,102,103,104,105,106].…”

Section: Natural Polymers For Tissue/organ 3d Bioprintingmentioning

confidence: 99%

Natural Polymers for Organ 3D Bioprinting

et al. 2018

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Three-dimensional (3D) bioprinting, known as a promising technology for bioartificial organ manufacturing, has provided unprecedented versatility to manipulate cells and other biomaterials with precise control their locations in space. Over the last decade, a number of 3D bioprinting technologies have been explored. Natural polymers have played a central role in supporting the cellular and biomolecular activities before, during and after the 3D bioprinting processes. These polymers have been widely used as effective cell-loading hydrogels for homogeneous/heterogeneous tissue/organ formation, hierarchical vascular/neural/lymphatic network construction, as well as multiple biological/biochemial/physiological/biomedical/pathological functionality realization. This review aims to cover recent progress in natural polymers for bioartificial organ 3D bioprinting. It is structured as introducing the important properties of 3D printable natural polymers, successful models of 3D tissue/organ construction and typical technologies for bioartificial organ 3D bioprinting.

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Evaluation of fibrin-gelatin hydrogel as biopaper for application in skin bioprinting: An in-vitro study

Abstract: The suggested optimized hydrogel was a suitable candidate as a biopaper for skin bioprinting technology.

Cited by 27 publications

References 19 publications

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Advances in the Research of Bioinks Based on Natural Collagen, Polysaccharide and Their Derivatives for Skin 3D Bioprinting

Natural Polymers for Organ 3D Bioprinting

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