Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications

Campos, Fernando; Bonhome-Espinosa, Ana B.; Chato‐Astrain, Jesús; Sánchez-Porras, David; García-García, Óscar Darío; Carmona, Rafael Marfil; López–López, Modesto T.; Alaminos, Miguel; Carriel, Víctor; Rodríguez, Ismael Ángel

doi:10.3389/fbioe.2020.00596

Cited by 47 publications

(41 citation statements)

References 73 publications

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“…Currently, technical progress in tissue engineering allows to design and generate highly complex 3D substitutes for tissue repair and replacement [ 52 , 53 ]. However, most of them are based on the use of synthetic biomaterials which are less biocompatible and lack pro-regenerative properties [ 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

García-García

Soury

González-Quevedo

et al. 2021

IJMS

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Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.

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Section: Discussionmentioning

confidence: 99%

Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

García-García

Soury

González-Quevedo

et al. 2021

IJMS

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“…Self-gelling properties and adjustable mechanical stability [220,221] of agarose gels are crucial for their use. For example, non-toxic [222] and biodegradable agarose gels have been effectively used in implantation surgery [219], wound healing, cartilage [223], cardiac, bone and nervous system [224], and regeneration as well as skin tissue engineering [225,226]. These directions are based on tunable features of agarose, which can result in adjustable characteristics similar to native tissues [225].…”

Section: Patentsmentioning

confidence: 99%

Progress in Modern Marine Biomaterials Research

et al. 2020

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The growing demand for new, sophisticated, multifunctional materials has brought natural structural composites into focus, since they underwent a substantial optimization during long evolutionary selection pressure and adaptation processes. Marine biological materials are the most important sources of both inspiration for biomimetics and of raw materials for practical applications in technology and biomedicine. The use of marine natural products as multifunctional biomaterials is currently undergoing a renaissance in the modern materials science. The diversity of marine biomaterials, their forms and fields of application are highlighted in this review. We will discuss the challenges, solutions, and future directions of modern marine biomaterialogy using a thorough analysis of scientific sources over the past ten years.

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“…Among the different biomaterials that showed preclinical and clinical usefulness for the generation of human tissue substitutes, fibrin-agarose hydrogels show excellent biocompatibility and biomechanical properties and its porous fibrillar pattern allows diffusion and interchange of oxygen and nutrients [ 2 , 3 , 5 , 6 ]. Fibrin-agarose was previously used to generate bioartificial substitutes of the human cornea [ 7 ], sclera [ 8 ], oral mucosa [ 9 ], palate [ 10 ], nerve [ 11 , 12 ], cartilage [ 13 ] and skin [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering

et al. 2020

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Background Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. Conclusions These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.

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Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications

Cited by 47 publications

References 73 publications

Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

Progress in Modern Marine Biomaterials Research

Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering

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