Evaluation of feeding and mixing conditions for fractional precipitation of paclitaxel from plant cell cultures

Lee, Da-Hye; Kim, Seul-Gi; Mun, Sungyong; Kim, Jin-Hyun

doi:10.1016/j.procbio.2010.04.006

Cited by 9 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Large-scale bioreactors are being considered for stable and long-term production and accumulation of Taxol (Lee et al, 2010). Khosroushahi et al (2006) employed a two-stage suspension culture method using Gamborg's B5 medium as basal medium and explored different inducing factors, similar to the current study in attempts to improve Taxol production in suspension culture of T. baccata.…”

Section: Effects Of Changes In the Concentrations Of Each Macronutrientmentioning

confidence: 96%

“…Using biotechnological advances, semi-or total synthesis of Taxol has been carried out by culturing endophytes and in cell suspension cultures. Yielding Taxol in bioreactors without destroying plant in its natural habitat for continuous and abundant production is being considered the best long-term alternative approach for taxoid production (Lee et al, 2010). Many scientists prefer two-phase culture along with the use of elicitors to increase the productivity along with maintaining the integrity of the cells (Dornenburg and Knorr, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Gauchan¹,

Bhuju²,

Lamichhane³

et al. 2020

J Appl Pharm Sci

View full text Add to dashboard Cite

Taxus wallichiana, an endangered Taxus species native to Nepal, is a source of Taxol (paclitaxel), an anticancer drug, which if produced via plant cell culture in vitro not only reduces the pressure on natural habitat but also allows continuous production throughout the seasons. Gamborg's B5 medium was the culture medium to develop callus and suspension culture. Quantification of Taxol was carried out via high performance liquid chromatography (HPLC) using C18 column, water-acetonitrile mobile phase, and 1 ml/minute flowrate at 227 nm wavelength. Genetic variation among population was determined using the POPGEN32, version 1.31. Best callus response was in medium with 1 mg/l 2,4-D for both stem and needle explants. For cell suspension culture, callus from needle explants showed better response compared to stem. Fructose and sucrose proved to be the best carbon sources. Cell count increased as carbon concentration increased. 2,4-D at low concentration (<2 mg/l) and naphthaleneacetic acid at high concentration (>5 mg/l) showed higher cell growth rate. Inoculum volume was directly proportional to cell count. Extra addition of macronutrients had inhibitory effect on cell count, but the effect was reversed for MgSO 4 . Using HPLC, for bark, the highest Taxol yield was 0.057 mg/g distilled water (DW), while for needle was 0.056 mg/g DW. For suspension cultured HPLC samples, Taxol yield ranged at 0.004%. Size range of amplified Deoxyribonucleic acids was between 200 and 2,000 bp. Thus, this investigation generated a concrete data for Taxus population of Nepal providing efficient protocols for establishment of callus and cell suspension culture with the aim of producing Taxol in in vitro conditions.

show abstract

Section: Effects Of Changes In the Concentrations Of Each Macronutrientmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Gauchan¹,

Bhuju²,

Lamichhane³

et al. 2020

J Appl Pharm Sci

View full text Add to dashboard Cite

show abstract

“…Therefore, investigating the alternative and non-destructive methods especially biotechnological approaches to improve Taxol productivity has been increased recently. Nowadays, large-scale plant cell culture is considered as one of the most promising, stable and long-term methods to produce taxoids [6,7]. In the most previous studies, media optimizitions for improving biomass production in the cell cultures of various Taxus species have been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the basal medium for improving production and secretion of taxanes from suspension cell culture of Taxus baccata L

2012

View full text Add to dashboard Cite

Background and purpose of the studyTaxol is one of the most effective anticancer drugs that isolated from Taxus sp. due to the slow growth of Taxus trees and low concentration of Taxol in the tissues, the biotechnological approaches especially plant cell culture have been considered to produce Taxol in commercial scale.MethodsWe investigated the effects of basal medium type used in culture media on production of Taxol and other taxane compounds from cell suspension culture of T. baccata L. Briefly, five commonly basal media including Gamborg, Murashige and Skoog, Woody Plant, Schenk and Hildebrandt, and Driver and Kuniyuki medium were used for preparing separate suspension culture media. The intra- and extra-cellular yields of taxanes were analyzed by using HPLC after 21 days period of culturing.ResultsThe yields of taxanes were significantly different for the cultures prepared by different basal media. Moreover, the effects of basal medium on the yield of products differed for varius taxane compounds. Maximum yields of Baccatin III (10.03 mgl-1) and 10-deacetyl baccatin III (4.2 mgl-1) were achieved from the DKW basal media, but the yield of Taxol was maximum (16.58 mgl-1) in the WPM basal media. Furthermore, the secretion of taxanes from the cells into medium was also considerably affected by the type of basal medium. The maximum extra-cellular yield of Taxol (7.81 mgl-1), Baccatin III (5.0 mgl-1), and 10-deacetyl baccatin III (1.45 mgl-1) were also obtained by using DKW basal medium that were significantly higher than those obtained from other culture media.

show abstract

Enhanced taxane production and secretion from Taxus baccata cell culture by adding dimethylsulfoxide

Kajani

Moghim

Mofid

2012

Biotech and App Biochem

View full text Add to dashboard Cite

The ability of an aprotic solvent, dimethylsulfoxide (DMSO), to induce taxane synthesis and release from cell suspension culture of Taxus baccata was examined. The results showed that applying DMSO in optimal conditions not only led to enhancement in taxane excretion from the cells but also led to improvement in taxol synthesis. Maximum yields for taxol [3.34 mg/g dry cell weight (DCW)] were achieved by adding 5% DMSO to the culture at the late-exponential phase of cell growth (day 14) and culturing for 21 days, which was 2.26-fold of that for the control (1.48 mg/g DCW). However, adding 5% DMSO did not affect the yield of 10-deacetyl baccatin III and baccatin III. This condition also resulted in maximum extracellular taxane (4.86 mg/L); this value was 2.82-fold higher than that in the control (1.72 mg/L). We demonstrated that the late-exponential phase of cell growth could be the best time to add elicitor for maximizing the yield of taxol. Adding DMSO at earlier times (days 1 and 7) or in other concentrations (1% and 3%) had negative effects on taxane synthesis. Overall results suggest that DMSO has good potential to enhance synthesis and release taxol from cell suspension culture of T. baccata L.

show abstract

Evaluation of feeding and mixing conditions for fractional precipitation of paclitaxel from plant cell cultures

Cited by 9 publications

References 14 publications

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Optimization of the basal medium for improving production and secretion of taxanes from suspension cell culture of Taxus baccata L

Enhanced taxane production and secretion from Taxus baccata cell culture by adding dimethylsulfoxide

Contact Info

Product

Resources

About