Evaluation of Fatty Acid Composition and Antimicrobial Activity of Eight Medicinal Plants from Kashmir

Nengroo, Zubair Rehman; Rauf, Abdur; Danish, Mohammad; Dar, Muzammil Sharief

doi:10.13005/ojc/360107

Cited by 10 publications

(2 citation statements)

References 24 publications

(25 reference statements)

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“…The results of this assay are presented in Table 4. All most all the methanol and ethanol extract were showed significant Biofilm reduction as compared to negative control except for ethanol and methanol extract of Taza The Indian Tea ++ +++ DISCUSSION: Green tea is one of the most popular beverages worldwide due to its healthpromoting properties, which reflect the presence of antioxidants and also antimicrobial substances that are active against various Gram-positive and Gramnegative bacteria [12][13][14][15][16] . Both activities are mainly due to polyphenols, the most abundant of which is the catechins, particularly epigallocatechin gallate (EGCG), representing 50-80% of the total catechin content 17 .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2022

IJPSR

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The emergency to find a solution to multidrug resistance of bacteria due to the abuse of antibiotics leads to the search for new antibacterial pathways. Quorum sensing (QS), or bacterial cell to cell communication, is a cell-density-dependent bacterial response. It is mediated by hormone-like compounds called autoinducers (AIs). QS-dependent regulation of gene expression controls various phenotypes, including bioluminescence, biofilm formation, drug resistance, virulence factors expression, and motility. Therefore, the inhibition of QS is considered a new promising target of antimicrobial pathways as anti-virulence compounds that can repress the gene expression of QS. Green tea is one of the most commonly consumed teas in the world. Green tea extract is also a great source of compounds like polyphenols, amino acids, enzymes, pigments, and carbohydrates. Hence, green tea has recently received considerable attention as a new source of safe and effective QS inhibitory substances. In the present study, methanolic and ethanolic various tea extracted were prepared and tested to inhibit quorum sensing mediated bacterial virulence factors such as anti-swarming, and antibiofilm potential against organism Pseudomonas aeruginosa. Quorum sensing inhibition against Chromobacterium violaceum MTCC 2656 has been carried out. The study's outcome shows that most of the methanolic and ethanolic extracts of various tea products show significantly Anti-quorum sensing Potential up to 83% swarming inhibition with Pseudomonas aeruginosa, inhibition violaceum pingment and biofilm disruption were also quite high in most of the Tea extract of ethanol and methanol. INTRODUCTION:The problem of multidrugresistant bacteria is becoming a worldwide concern nowadays. This pathogenic organism became resistant to a large range of antibiotics that possess a problem with treating various diseases 1 . Antibacterial drugs are the foremost effective of all QUICK RESPONSE CODE

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Section: Resultsmentioning

confidence: 99%

Untitled

2022

IJPSR

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“…Linoleic acid (C18:2) and eicosadienoic acid (C20:2) are a kind of polyunsaturated fatty acid that are present in the AAE. Linoleic acid has antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli [ 50 , 54 ]. Eicosadienoic acid methyl ester, another unsaturated fatty acid present in the AAE, also has antibacterial activity towards Gram-positive bacteria ( Bacillus subtilis, B. cereus, and Staphylococcus aureus ) and Gram-negative bacteria ( E. coli, P. aeruginosa , and S. typhi ) [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Unveiling the Potential of Algal Extracts as Promising Antibacterial and Antibiofilm Agents against Multidrug-Resistant Pseudomonas aeruginosa: In Vitro and In Silico Studies including Molecular Docking

El-Sapagh,

El-Shenody,

Pereira

et al. 2023

Plants

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Multidrug-resistant Pseudomonas aeruginosa poses a global challenge due to its virulence and biofilm-forming ability, leading to persistent infections. This study had a dual focus: first, it aimed to investigate the biofilm activity and antibiotic resistance profiles of Pseudomonas aeruginosa isolates obtained from a fish-rearing farm. Second, it explored the potential of algal extracts as effective antibacterial and antibiofilm agents. The study analyzed 23 isolates of P. aeruginosa from the farm, assessing antibiotic resistance and biofilm formation. The antimicrobial and antibiofilm activities of two algal extracts, Arthrospira platensis (cyanobacteria) acetone extract (AAE) and Polysiphonia scopulorum (Rhodophyta) methanol extract (PME), were tested individually and combined (COE). The effects on biofilm-related gene expression were examined. AAE, PME, and COE were evaluated for antimicrobial and antibiofilm properties. Biofilm-related gene expression was measured and the extracts were analyzed for physicochemical properties and toxicity. Most P. aeruginosa isolates (86.9%) were antibiotic-resistant and formed biofilms. AAE, PME, and COE displayed promising antibacterial and antibiofilm effects, with COE being particularly effective. COE reduced a key biofilm-related gene expression. The fatty acid content (56% in AAE and 34% in PME) correlated with the effects. Specific compounds, such as phytol, bromophenol, and dihydroxy benzaldehyde, contributed to the activities. The extracts showed favorable characteristics and interactions with FabZ protein amino acids. This study suggests the potential of algal extracts as antibacterial and antibiofilm agents against drug-resistant infections. Further exploration in clinical applications is warranted.

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