Evaluation of faecal bile acid profiles by HPLC after using disposable solid-phase columns

Vescina, M.C.; Mamianetti, A; Vizioli, Nora Matilde; Lucangioli, Silvia; Rodríguez, V.; Orden, A La; Garrido, Delia; Carducci, C. N.

doi:10.1016/0731-7085(93)80120-p

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…To isolate the sodium salts of taurine conjugates as crystalline products, the reaction mixtures diluted with water were loaded on Sep-Pak C 18 cartridges for reversed-phase solid extraction (13)(14)(15). The cartridges were washed successively with water and 20% aqueous EtOH to remove excess reagents, contaminants, and inorganic salts.…”

Section: Resultsmentioning

confidence: 99%

An improved synthesis of taurine‐ and glycine‐conjugated bile acids

Momose

Tsubaki

Iida

et al. 1997

Lipids

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A simple and efficient method for the synthesis of taurine- and glycine-conjugated bile acids is described. The condensation reaction was achieved by the simple mixing of unconjugated bile acid (1.0 eq.), taurine (2.0 eq.) (or glycinate ester), diethyl phosphorocyanidate (1.2 eq.) in the presence of triethylamine at room temperature for 30-60 min. Sample clean-up was effected by the use of a prepacked Sep-Pak C18 cartridge for reversed-phase solid extraction or by direct recrystallization, yielding the desired taurine and glycine conjugates in 89-93 and 92-96% isolated yields, respectively.

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Section: Resultsmentioning

confidence: 99%

An improved synthesis of taurine‐ and glycine‐conjugated bile acids

Momose

Tsubaki

Iida

et al. 1997

Lipids

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“…In this sense, high-performance liquid chromatography (HPLC) has been a popular technique applied in the individual analysis of BA in biological www.intechopen.com Cholestasis 8 fluids. The HPLC-methods reported involve two chromatographic systems to determine the complete BA profile, one for free BA and the other system for BA derivatives (Vescina et al, 1993). Unfortunately, the combination of HPLC with UV-detection, limited the sensitivity of the analysis due to the fact that BA have little chromophorore groups in their molecules (Fig.…”

Section: Separative Techniquesmentioning

confidence: 99%

Intrahepatic Cholestasis of Pregnancy: The Usefulness of Serum Bile Acid Profile for Diagnosis and Treatment

Lucangioli¹,

Tripodi²

2012

Cholestasis

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Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

Duff

2000

Encyclopedia of Analytical Chemistry

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Within the realm of pharmaceutical drug analyses, two techniques currently dominate the field. High‐performance liquid chromatography (HPLC) is at the forefront, followed somewhat distantly by gas chromatography (GC). HPLC is more prevalent than GC owing mainly to the heat‐labile nature of the majority of drug substances. Still GC continues to play a very active role. In comparison to HPLC and GC, combined, the frequency of use of other techniques is quite small. Perhaps the major reason for this is that chromatography not only involves qualitative and quantitative detection of targeted species, but also provides a medium for isolating these analytes from potential interfering impurities prior to measurement. The proficiency of this combination is unparalleled by any other blending of means to clean up a sample and detect the target compound. These more prominent techniques are often complementary, although each has its own strengths and limitations. General guidelines are given to assist the chromatographer to elect HPLC and/or GC for a particular separation. Considerations for choosing hardware type and sizes, solid support and bonded phase are presented. Particular emphasis is placed on the latest, which is of primary importance for obtaining rugged methods. The reader is directed towards optimum phase selection based on the compound of interest's functional group type, or by its drug activity classification (e.g. barbiturate, cardiovascular, etc.). Assistance in developing new methods frequently arises when starting with an existing published method for a compound with similar structure. This article provides 514 references for more in‐depth separation information. In addition, background knowledge on bonded‐phase chemistry, chromatographic modes of separation, approaches to chromatographic chiral drug discrimination and processes for developing rugged methods is contributed.

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Evaluation of faecal bile acid profiles by HPLC after using disposable solid-phase columns

Cited by 4 publications

References 22 publications

An improved synthesis of taurine‐ and glycine‐conjugated bile acids

An improved synthesis of taurine‐ and glycine‐conjugated bile acids

Intrahepatic Cholestasis of Pregnancy: The Usefulness of Serum Bile Acid Profile for Diagnosis and Treatment

Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

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