Evaluation of extracts prepared from 16 plants used in Yao ethnomedicine as potential anticancer agents

Almosnid, Nadin; Zhou, Xiaolei; Jiang, Lufang; Ridings, Amy; Knott, Deborah L.T.; Wang, Shuo; Fan, Wei; Altman, Elliot; Gao, Ying; Jin, Miao

doi:10.1016/j.jep.2017.09.032

Cited by 12 publications

(6 citation statements)

References 53 publications

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“…Recent studies on the use of natural herbal-derived anticancer agents have shown that natural products could prove promising as anticancer agents with fewer side effects [ 7 ]. Among the natural agents studied, plant-derived secondary metabolites have been shown to exhibit potent anticancer activity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Girinimbine Inhibits the Proliferation of Human Ovarian Cancer Cells In Vitro via the Phosphatidylinositol-3-Kinase (PI3K)/Akt and the Mammalian Target of Rapamycin (mTOR) and Wnt/β-Catenin Signaling Pathways

Xin

Muer

2018

Med Sci Monit

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BackgroundWorldwide, ovarian cancer is increasing in prevalence and has a high mortality rate. Girinimbine is a carbazole alkaloid isolated from Murraya koenigii (the curry tree) and is used in Chinese herbal medicine. The aim of this study was to evaluate the effects of girinimbine on cell proliferation, cell migration, and apoptosis in human ovarian cancer cells in vitro.Material/MethodsA human ovarian cancer cell line panel, which included SKOV3 cells and the SV40 immortalized normal human ovarian cell line, were treated with increasing doses of girinimbine. Cell proliferation was evaluated using the MTT assay. Confocal immunofluorescence using 4′,6-diamidino-2-phenylindole (DAPI), Annexin-V, and propidium iodide (PI) were used to measure cell apoptosis. Cell migration and invasion were determined by transwell assays. Protein expression was determined by Western blot.ResultsGirinimbine inhibited SKOV3 ovarian cancer cell proliferation in a dose-dependent manner. The half maximal inhibitory concentration (IC50) of grinimbine was 15 μM for the SKOV3 cells, and 120 μM for the SV40 cells. Grinimbine treatment resulted in apoptosis of SKOV3 cells, from 2.2% in untreated cells to 58.8% at a dose of 30 μM, which was associated with an increase in the Bax/Bcl-2 ratio. Girinimbine inhibited cell migration and invasion of the SKOV3 cancer cells in vitro and inhibited the PI3K/AKT/mTOR and Wnt/β-catenin signaling pathways.ConclusionsGirinimbine, a carbazole alkaloid used in Chinese herbal medicine, inhibited the proliferation and cell migration of human ovarian cancer cells in vitro, in a dose-dependent manner, via the PI3K/Akt/mTOR and Wnt/β-catenin signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Girinimbine Inhibits the Proliferation of Human Ovarian Cancer Cells In Vitro via the Phosphatidylinositol-3-Kinase (PI3K)/Akt and the Mammalian Target of Rapamycin (mTOR) and Wnt/β-Catenin Signaling Pathways

Xin

Muer

2018

Med Sci Monit

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“…In previous studies, the anti-growth activity of extracts was determined through an antigrowth assay with expressed results as IC 50 values. [10,11] Moreover, some studies have also reported the antioxidant, antibacterial, antimalarial, or enzyme inhibitory properties of the selected plants. [5,14,21] In this study, we went beyond simply revealing the growth inhibitory activity of the selected plants to understand how this anti-growth activity was manifested.…”

Section: Anti-growth Activity Of the Extractsmentioning

confidence: 99%

“…Several studies have explored whether the resulting cell death is induced by apoptosis. [10,11] Anatolia boasts an impressive floristic diversity, with over 12,000 natural vascular plant species and a remarkable endemism rate of more than 30 %. [12] Despite the rich flora, studies investigating the anticancer properties of a multitude of plant species are relatively scarce.…”

Section: Introductionmentioning

confidence: 99%

“…reported the results of a collaborative screening of 7,500 South African plants, while Mazzio and Soliman [9] reported tumoricidal activity of 374 natural extracts, mostly from plants used worldwide as anticancer complementary and alternative medicines. Several studies have explored whether the resulting cell death is induced by apoptosis [10,11] …”

Section: Introductionmentioning

confidence: 99%

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Plants from Northwestern Anatolia Display Selective Cytotoxicity and Induce Mitotic Catastrophe: A Study on Anticancer and Genotoxic Activities

Sarimahmut

Çelikler

2023

Chemistry & Biodiversity

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Anatolia is rich in floristic diversity with a high rate of endemism. Eight plant species from northwestern Anatolia were evaluated for their anti‐growth properties in two malignant (MCF‐7 and MDA‐MB‐231) and a non‐malignant (MCF‐10A) breast cell line. The two most active extracts, Achillea multifida (AME) and Astragalus sibthorpianus (ASE), induced apoptotic cell death in all cell lines. The major phenolic compounds in AME were identified as chlorogenic acid, and catechins in ASE. ASE displayed selective cytotoxicity against breast cancer cells, with DNA damage repair in non‐malignant cells contributing to its selectivity. Conversely, AME induced DNA damage in a time‐dependent manner and displayed a dual dose‐dependent biological activity, resulting in mitotic catastrophe and apoptosis at different doses. Most plant species exhibited moderate to strong cytotoxicity, highlighting their medicinal and economic potential and the need for their protection.

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“…M. leucadendra, commonly known as Cajuput, is a tree native to Australia and Southeast Asia. Traditionally, the leaves are used in the treatment of colds, fever, skin lesions, and to relieve rheumatism [16][17][18]. Cajuput oil is utilized in hygienic and medicinal products to relieve cough, colds, and as a disinfectant.…”

Section: Introductionmentioning

confidence: 99%

Protective Role of Casuarinin from Melaleuca leucadendra against Ethanol-Induced Gastric Ulcer in Rats

et al. 2019

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Gastric ulcer is a major health problem. Current treatment options of gastric ulcer, including antagonists of histamine H2 receptor and inhibitors of the proton pump, do not cure gastric ulcers, but only provide temporary relief of symptoms and can be associated with severe side effects. The lack of effective and safe medications for this global health problem urges for the discovery of novel classes of compounds with potent activity and an acceptable safety profile. Ethanol-induced ulceration in rats was used to evaluate the gastroprotective activity of casuarinin, an ellagitannin isolated from Melaleuca leucadendra. Casuarinin (25, 50, and 100 mg/kg) reduced the ulcer area by 45, 78, and 99%, respectively, compared with the ulcer group. Casuarinin (100 mg/kg) increased mucin content by 1.8-fold and reduced acidity by 42%. At the same dose, it also increased the levels of reduced glutathione by 194%, catalase by 586%, and prostaglandin E2 to its normal level. In contrast, it attenuated the ethanol-increased levels of malondialdehyde by 56%, TNF-α by 58%, and caspase-3 by 87%. Histological findings demonstrated that casuarinin exhibited a protective effect against tissue alterations in response to the ethanol-induced ulcer. Casuarinin suppressed the immunoexpression of nuclear factor-kappa B, cyclooxygenase-2, and inducible nitric oxide synthase to their normal values. It also induced the expression of heat shock protein-70, reaching up to 4.9-fold in comparison with the ulcer group. The potent gastroprotective effect of casuarinin was thus attributed to its anti-inflammatory, antioxidant, and antiapoptotic effects. Our results suggest the potential application of casuarinin as an antiulcer agent from natural sources.

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Evaluation of extracts prepared from 16 plants used in Yao ethnomedicine as potential anticancer agents

Cited by 12 publications

References 53 publications

Girinimbine Inhibits the Proliferation of Human Ovarian Cancer Cells In Vitro via the Phosphatidylinositol-3-Kinase (PI3K)/Akt and the Mammalian Target of Rapamycin (mTOR) and Wnt/β-Catenin Signaling Pathways

Girinimbine Inhibits the Proliferation of Human Ovarian Cancer Cells In Vitro via the Phosphatidylinositol-3-Kinase (PI3K)/Akt and the Mammalian Target of Rapamycin (mTOR) and Wnt/β-Catenin Signaling Pathways

Plants from Northwestern Anatolia Display Selective Cytotoxicity and Induce Mitotic Catastrophe: A Study on Anticancer and Genotoxic Activities

Protective Role of Casuarinin from Melaleuca leucadendra against Ethanol-Induced Gastric Ulcer in Rats

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