Evaluation of extraction method on the chemical composition in Apeiba tibourbou Aubl′s extracts

Martins, Frederico Severino; Conceição, Edemilson Cardoso da

doi:10.4103/0973-1296.153091

Cited by 10 publications

(8 citation statements)

References 11 publications

(21 reference statements)

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“…The most widely used method for extracting capsaicin is based on extraction with hexane, which is very toxic and produces residual solvent. [ 10 11 12 ]…”

Section: Introductionmentioning

confidence: 99%

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)

Martins¹,

Borges²,

Ribeiro³

et al. 2017

Phcog Mag

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Introduction:The objective of this study was to compare three capsaicin extraction methods: Shoxlet, Ultrasound-assisted Extraction (UAE), and Shaker-assisted Extraction (SAE) from Habanero pepper, CNPH 15.192.Materials and Methods:The different parameters evaluated were alcohol degree, time extraction, and solid–solvent ratio using response surface methodology (RSM).Results:The three parameters found significant (p < 0.05) were for UAE and solvent concentration and extraction time for SAE. The optimum conditions for the capsaicin UAE and SAE were similar 95% alcohol degree, 30 minutes and solid–liquid ratio 2 mg/mL. The Soxhlet increased the extraction in 10–25%; however, long extraction times (45 minutes) degraded 2% capsaicin.Conclusion:The extraction of capsaicin was influenced by extraction method and by the operating conditions chosen. The optimized conditions provided savings of time, solvent, and herbal material. Prudent choice of the extraction method is essential to ensure optimal yield of extract, thereby making the study relevant and the knowledge gained useful for further exploitation and application of this resource.SUMMARY Habanero pepper, line CNPH 15.192, possess capsaicin in higher levels when compared with others speciesHigher levels of ethanolic strength are more suitable to obtain a higher levels of capsaicinBox-Behnken design indicates to be useful to explore the best conditions of ultrasound assisted extraction of capsaicin. Abbreviations used: Nomenclature UAE: Ultrasound-assisted Extraction; SAE: Shaker-assisted Extraction.

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“…The most widely used method for extracting capsaicin is based on extraction with hexane, which is very toxic and produces residual solvent. [ 10 11 12 ]…”

Section: Introductionmentioning

confidence: 99%

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)

Martins¹,

Borges²,

Ribeiro³

et al. 2017

Phcog Mag

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“…Ultrasonic-assisted extraction is an efficient way to obtaining rosmarinic acid enriched alcohol extracts from plant material. [ 30 ]…”

Section: Discussionmentioning

confidence: 99%

Inhibition of glycation-induced cytotoxicity, protein glycation, and activity of proteolytic enzymes by extract from Perovskia atriplicifolia Roots

Miroliaei¹,

Aminjafari²,

Ślusarczyk³

et al. 2017

Phcog Mag

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Background:Protein glycation and glycotoxicity belong to the main oxidative-stress related complications in diabetes. Perovskia species are used in Asian folk medicine as antidiabetic herbs.Objective:The aim of this study was to verify the ability of the methanolic extract from Perovskia atriplicifolia Benth. roots to diminish glycation of albumin and to prevent cell damage in vitro. Furthermore, we tested the extract for in vitro antioxidant activity and inhibition of elastase and collagenase.Material and Methods:The aqueous methanol extract was analyzed by UHPLC-MS for the content of polyphenols and terpenoids. The prevention of glycated albumin-induced cell damage was tested in four mammalian cell lines (peripheral blood mononuclear cells, human embryonic kidney cells – HEK293, normal human fibroblasts, and Chinese hamster ovary cells) with the 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazoly)-3-(4-sulfophenyl) tetrazolium assay.Results:Glycated albumin is significantly more toxic than native human serum albumin (LC50 from 35.00 to 48.34 μg/mL vs. 5.47–9.10 μg/mL, respectively). The extract, rich in rosmarinic acid (344.27 mg/g dry mass), mitigated the glycated albumin toxicity, and increased glycated albumin-treated cell survival by more than 50%. The inhibition of advanced glycation endproduct formation was confirmed by monitoring conformational changes. The free radical scavenging activity was higher than Trolox and metal reducing power was one-third to half that of ascorbic acid. The activity of elastase and collagenase was inhibited by 54.75% ± 6.87% and 60.03% ± 7.22%, respectively.Conclusions:The results confirm antiglycative and antiglycotoxic potential of Perovskia root and its traditional antidiabetic use. The high activity can be attributed to rosmarinic acid abundance.SUMMARY Perovskia is a small genus of aromatic shrubby plants growing in arid regions of Central and South Asia. Different parts are used in folk medicine as antiparasitic, anti-infectious and antidiabetic remedy. Here, we have studied the extract from roots for inhibition of: glycation-induced cytotoxicity, human serum albumin glycation, inflammation-related enzymes, as well as for antioxidant activity. Result: the extract from P. atriplicifolia roots inhibited protein glycation and AGE-induced toxicity in cell cultures. The mechanism is likely to rely on the antioxidant activity of high content of rosmarinic acid. Abbreviations used: AGE: advanced glycation end-products; DPPH: 2,2-diphenyl-1-picrylhydrazyl; HSA: human serum albumin.

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“…Furthermore, the vigorous homogenization process performed in Bligh and Dyer (1959) method making the lipid extraction more efficient (Brum et al, 2009). Another advantage of this method includes less time required (Martins et al, 2015). On the other hand, the Soxhlet method (1879) is based on use of a single solvent resulting only in non-polar lipid extraction (Lu et al, 2015).…”

Section: Total Lipid Contentmentioning

confidence: 99%

Comparative Study on Total Lipid Quantification and Oxidative Rancidity Determination in Freshwater Fish Species with Different Fat Levels

Alcântara¹,

Azevedo²,

Mársico³

et al. 2015

JFS

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The aim of this study was to investigate the efficiency of different extraction methods for total lipid determination and oxidative rancidity evaluation in freshwater fish with different fat levels. Total lipid content was determined by Bligh and Dyer with slight modifications and Soxhlet methods. Malondialdehyde (MDA) quantification was determined by analytical methods with and without heat application. In both freshwater fish species, Bligh and Dyer method extracted greater total lipid content (P <0.05) than Soxhlet method. In addition, the heat method revealed higher MDA values (P <0.05) compared to cold method in both lean fish (Cichla ocellaris) and high-fat fish species (Piaractus brachypomus). We concluded that, regardless of the fat content in the freshwater fish species, the Bligh and Dyer method and the cold method used for oxidative rancidity were more efficient than Soxhlet method and heat technique, respectively.

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Evaluation of extraction method on the chemical composition in Apeiba tibourbou Aubl′s extracts

Cited by 10 publications

References 11 publications

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)

Inhibition of glycation-induced cytotoxicity, protein glycation, and activity of proteolytic enzymes by extract from Perovskia atriplicifolia Roots

Comparative Study on Total Lipid Quantification and Oxidative Rancidity Determination in Freshwater Fish Species with Different Fat Levels

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