2015
DOI: 10.5296/jfs.v4i1.8362
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Comparative Study on Total Lipid Quantification and Oxidative Rancidity Determination in Freshwater Fish Species with Different Fat Levels

Abstract: The aim of this study was to investigate the efficiency of different extraction methods for total lipid determination and oxidative rancidity evaluation in freshwater fish with different fat levels. Total lipid content was determined by Bligh and Dyer with slight modifications and Soxhlet methods. Malondialdehyde (MDA) quantification was determined by analytical methods with and without heat application. In both freshwater fish species, Bligh and Dyer method extracted greater total lipid content (P <0.05) than… Show more

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Cited by 3 publications
(3 citation statements)
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References 42 publications
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“…To determine the lipid oxidation, the secondary products of lipid oxidation were measured using the UV-1800 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 532 nm by quantification of substances reactive to thiobarbituric acid (TBARS) at the various time points during storage using the methodology modified by Alcântara et al [32]. The results were expressed as mg of malonaldehyde (MDA) per gram of meat, based on a calibration curve.…”
Section: Methodsmentioning
confidence: 99%
“…To determine the lipid oxidation, the secondary products of lipid oxidation were measured using the UV-1800 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 532 nm by quantification of substances reactive to thiobarbituric acid (TBARS) at the various time points during storage using the methodology modified by Alcântara et al [32]. The results were expressed as mg of malonaldehyde (MDA) per gram of meat, based on a calibration curve.…”
Section: Methodsmentioning
confidence: 99%
“…Lipid oxidation was carried out during storage (d 0, 3, 6, and 9) of conventional and antibiotic-free chicken patties samples. The substances reactive to thiobarbituric acid ( TBARS ) were quantified by a spectrophotometer UV-1800 (Shimadzu Corporation, Kyoto, Japan) at 532 nm during several points of storage ( Alcântara et al, 2015 ). The results were expressed as mg of malonaldehyde ( MDA ) per kilogram of meat, based on a calibration curve.…”
Section: Methodsmentioning
confidence: 99%
“…After sterilization process, cooling process should be immediately done to prevent over cooking [5]. TBA number increases as the increase of temperature and time, it caused by fat oxidation in food product [21], [22]. TBA number for decayed NU is 0.5 mg/kg based on previous study.…”
Section: -4mentioning
confidence: 99%