Evaluation of exploration and risk assessment in pre-weaning mice using the novel cage test

Marques, Joana Montezano; Olsson, I. Anna S.; Ögren, Sven Ove; Dahlborn, K.

doi:10.1016/j.physbeh.2007.08.006

Cited by 27 publications

(17 citation statements)

References 29 publications

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“…Firstly, as noted by Bruce et al (2009), we did not observe significant reductions in birth weight in offspring who demonstrated a moderate (approximately 15%), yet significant reduction in H19 ICR methylation, but did observe postnatal growth deficiencies. Secondly, a growth restricted phenotype similar to SRS, was correlated with hypomethylation of the H19 ICR CTCF binding sites that was associated with reduced offspring growth in the period following weaning (Marques et al, 2008; Nicholson et al, 2009). Lastly, weight recovery was observed during the post-weaning period, similar to findings of Blissett et al (2001).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Preconception Paternal Alcohol Exposure on Epigenetic Remodeling of the H19 and Rasgrf1 Imprinting Control Regions in Mouse Offspring

Knezovich¹,

Ramsay²

2012

Front. Gene.

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Imprinted loci play a critical role in fetal development. Their expression is often regulated by CCCTC-binding factor (CTCF) protein binding at imprinting control regions (ICRs). Prenatal alcohol exposure has been shown to reduce global DNA methylation in the developing mouse fetus. This study explored the effect of preconception paternal alcohol exposure on DNA methylation at two paternally methylated ICRs (H19 and Rasgrf1) in the sperm of exposed males and somatic DNA of sired offspring. Significant reductions at the H19 CTCF 1 (p = 0.0027) and CTCF 2 (p = 0.0009) binding sites were observed in the offspring of ethanol-treated sires, which was significantly correlated with reduced weight at postnatal days 35–42 (p < 0.05). As birth weight was unaffected and growth was only delayed during the postnatal weaning period, with subsequent re-convergence, we hypothesize that this may be the result of a mental deficit causing delayed establishment of independent feeding following weaning and would explain why this effect is transient. No difference in DNA methylation was observed in the sperm of alcohol-exposed males, indicating that the transmission of the epigenetic signal at conception is not due to altered methylation, but may be the result of an RNA-mediated mechanism or altered chromatin remodeling.

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Section: Discussionmentioning

confidence: 99%

The Effect of Preconception Paternal Alcohol Exposure on Epigenetic Remodeling of the H19 and Rasgrf1 Imprinting Control Regions in Mouse Offspring

Knezovich¹,

Ramsay²

2012

Front. Gene.

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“…With this non-prejudiced multivariate design, several measures can be monitored, providing a behavioral profile in a single test situation, rather than focusing on any particular behavior (Meyerson et al, 2006). The MCSF test has an ethoexperimental and multivariate approach, and has been used for behavioral profiling in a number of studies (Alsio et al, 2009;Augustsson et al, 2005;Augustsson and Meyerson, 2004;Karlsson et al, 2009;Marques et al, 2008;Roman and Colombo, 2009;Roman et al, 2006Roman et al, , 2007. ''Ethoexperimental'' means that native forms of behavior, derived from the natural environment of the species studied, is taken into account.…”

Section: Introductionmentioning

confidence: 99%

The Multivariate Concentric Square Field Test Reveals Behavioral Profiles of Risk Taking, Exploration, and Cognitive Impairment in Mice Subjected to Traumatic Brain Injury

Ekmark‐Lewén

Lewén

Meyerson

et al. 2010

Journal of Neurotrauma

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There is a need for more efficient tests to evaluate functional outcome following experimental traumatic brain injury (TBI), reflecting deficits in cognitive, sensory, and motor functions that are seen in TBI patients. The Multivariate Concentric Square Field (MCSF) test is a relatively new behavioral model that measures exploration, risk taking, risk assessment, and shelter seeking, all of which are evolutionarily-conserved strategies for survival. The multivariate design enables scoring of different functional domains in a single test situation, with a free choice of optional environmental settings. Furthermore, repeated trials permits cognitive effects to be measured. In the present study, 11 anesthetized C57BL6 mice received controlled cortical injury (CCI) (0.5 mm and 3.3 m/sec) over the right parietal cerebral cortex or sham surgery (n = 12). Naïve mice (n = 12) not subjected to any surgical procedure were also included. The animals were evaluated in the MCSF test at 2 and 7 days post-surgery, and behavioral profiles were analyzed. The results revealed differences in risk taking and explorative behavior between the sham animals and the animals subjected to trauma. Animals subjected to trauma were characterized by taking more risks and had a higher level of exploration activity, but they sought less shelter. Repeated exposure to the MCSF caused a general decrease in activity in the naïve and sham group, while a more specific behavioral impairment was seen in injured mice, suggesting cognitive dysfunction. We submit that the MCSF test is a useful complementary tool for functional outcome evaluation in experimental TBI.

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“…While this may present an advantage in exploration-based paradigms, by introducing a chance to predict adult exploratory phenotypes (e.g. Marques et al, 2008), in tests which require animals to focus on one environmental cue, this higher tendency to explore may impair learning and performance. Therefore, reducing the novelty of the test environment is crucial when testing rodents at young ages.…”

Section: Discussionmentioning

confidence: 99%

Performance of juvenile mice in a reach-to-grasp task

Marques¹,

Olsson²

2010

Journal of Neuroscience Methods

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Evaluation of exploration and risk assessment in pre-weaning mice using the novel cage test

Cited by 27 publications

References 29 publications

The Effect of Preconception Paternal Alcohol Exposure on Epigenetic Remodeling of the H19 and Rasgrf1 Imprinting Control Regions in Mouse Offspring

The Effect of Preconception Paternal Alcohol Exposure on Epigenetic Remodeling of the H19 and Rasgrf1 Imprinting Control Regions in Mouse Offspring

The Multivariate Concentric Square Field Test Reveals Behavioral Profiles of Risk Taking, Exploration, and Cognitive Impairment in Mice Subjected to Traumatic Brain Injury

Performance of juvenile mice in a reach-to-grasp task

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