Performance of juvenile mice in a reach-to-grasp task

Marques, Joana Montezano; Olsson, I. Anna S.

doi:10.1016/j.jneumeth.2010.08.016

Cited by 7 publications

(9 citation statements)

References 17 publications

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“…Standard rodent pellets and water were provided ad libitum except during test period (W5-postnatal day 26) when food restriction was introduced. We used a training box of the same dimensions and followed procedures as established by Marques and Olsson ( 2010 ). Briefly, food restriction schedule provided enough food to maintain approximately 85 % of body weight.…”

Section: Methodsmentioning

confidence: 99%

“…We recorded all sessions with a conventional camcorder for further analysis. Performance was analyzed according to the quantitative measures established by Marques and Olsson ( 2010 ): Latency to the first reaching attempt (the single first time the mouse performed a reaching movement), reaching accuracy = (number of pellets retrieved/number of reaches) × 100, latency to retrieve one pellet (the latency of the first successful grasp on each testing day) and time of performance = latency to retrieve 5 pellets − latency to retrieve 1 pellet.…”

Section: Methodsmentioning

confidence: 99%

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The neostriatum: two entities, one structure?

et al. 2015

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The striosome (or patch) was first identified with anatomical techniques as neurons organized in a three-dimensional labyrinth inserted in and interdigitating the rest of neostriatum: the matrix. Striosome and matrix rapidly became known as two neuronal compartments expressing different biochemical markers, embryonic development and afferent and efferent connectivity. In spite of extensive intrinsic neuronal axonal and dendritic extensions supposed to exchange information between matrix and striosomes, evidence suggested the presence of independent areas. Here, we report that indeed these two areas do not exchange synaptic information. We used genetic expression of channel rhodopsin 2 carried by adeno-associated virus serotype 10 (AAVrh10) that only expresses in neurons of the matrix compartment. Whole-cell patch-clamp recordings of matrix neurons activated by light pulses consistently produced inhibitory postsynaptic currents (IPSCs), but the same manipulation did not evoke IPSCs in striosome neurons. The matrix contains both direct and indirect striatal output pathways. By targeting striatal matrix expression of designer receptors exclusively activated by a designer drug (DREADD) hM3di carried by AAVrh10, we were able to inhibit the matrix neuronal compartment of the dorsolateral striatum during performance of a learned single-pellet reach-to-grasp task. As expected, inhibition of matrix neurons by systemic administration of DREADD agonist clozapine-n-oxide interfered with performance of the learned task.Electronic supplementary materialThe online version of this article (doi:10.1007/s00429-015-1000-4) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The neostriatum: two entities, one structure?

et al. 2015

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“…We examined coordinated motor function of the reach-tograsp test that is more sensitive and clinically relevant (Marques and Olsson, 2010). In sham control and stroke animals, the suc-4 (Figure legend continued.) in responding to CTZ were sites 41 and 44, which were located in the same cortical column judged from the brain slice orientation in F. Several responding sites were 400 -600 m away from the stimulation site (*), implicating neurons in a different cortical column.…”

Section: Intranasally Delivered Ctz Stimulated Grafted Lmo3-ips-npcs mentioning

confidence: 99%

Optochemogenetic Stimulation of Transplanted iPS-NPCs Enhances Neuronal Repair and Functional Recovery after Ischemic Stroke

Tung

Wei

et al. 2019

J. Neurosci.

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Cell transplantation therapy provides a regenerative strategy for neural repair. We tested the hypothesis that selective excitation of transplanted induced pluripotent stem cell-derived neural progenitor cells (iPS-NPCs) could recapitulate an activity-enriched microenvironment that confers regenerative benefits for the treatment of stroke. Mouse iPS-NPCs were transduced with a novel optochemogenetics fusion protein, luminopsin 3 (LMO3), which consisted of a bioluminescent luciferase, Gaussia luciferase, and an opsin, Volvox Channelrhodopsin 1. These LMO3-iPS-NPCs can be activated by either photostimulation using light or by the luciferase substrate coelenterazine (CTZ). In vitro stimulations of LMO3-iPS-NPCs increased expression of synapsin-1, postsynaptic density 95, brain derived neurotrophic factor (BDNF), and stromal cell-derived factor 1 and promoted neurite outgrowth. After transplantation into the ischemic cortex of mice, LMO3-iPS-NPCs differentiated into mature neurons. Synapse formation between implanted and host neurons was identified using immunogold electron microscopy and patch-clamp recordings. Stimulation of transplanted cells with daily intranasal administration of CTZ enhanced axonal myelination, synaptic transmission, improved thalamocortical connectivity, and functional recovery. Patch-clamp and multielectrode array recordings in brain slices showed that CTZ or light stimulation facilitated synaptic transmission and induced neuroplasticity mimicking the LTP of EPSPs. Stroke mice received the combined LMO3-iPS-NPC/CTZ treatment, but not cell or CTZ alone, showed enhanced neural network connections in the peri-infarct region, promoted optimal functional recoveries after stroke in male and female, young and aged mice. Thus, excitation of transplanted cells via the noninvasive optochemogenetics treatment provides a novel integrative cell therapy with comprehensive regenerative benefits after stroke.

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“…There also are occasional evidences about a possibility of elaboration of the manipulatory foodprocuring skill in mice older than one month [6].…”

Section: Resultsmentioning

confidence: 99%

“…The terms of elaboration of inborn motor reactions in developing rats have been studied sufficiently well [1,2]. However, there are only a few works that deal with detection of possibilities of learning of rat pups [3][4][5] and mouse pups [6] of behavior tasks at the early terms of postnatal development. At the same time, the data are absent in literature about terms and dynamics of development of the lateral preference in using the anterior paws in rats at the period of early postnatal ontogenesis.…”

Section: Introductionmentioning

confidence: 99%

Formation of lateralization of manipulatory food-procuring behavior in the white rat early ontogenesis

Volnova

Kurzina

2013

J Evol Biochem Phys

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Study of peculiarities of formation of preference in use of one of forepaws at learning of the manipulatory food-procuring skill has been carried out at different stages of early ontogenesis of white rats. It was established that as early as at the 3-week age the rat pups were already able to learn getting food from a narrow test-tube; however, no expressed preference in using one of the extremities was revealed. After repeated testing of adult rats at the age of 4 months the number of ambidexters was significantly higher than in the control animal group. At learning of the 30-day old rat pups of this manipulatory skill, in the majority of the rat pups, no significant lateralization also was found in using the forepaw. At repeated testing of adult rats the amount of ambidexters did not differ from that in the control group of animals. The obtained results indicate the existence of critical periods in formation of lateral preference in performance of the manipulatory food-procuring skill. There also was shown effect of learning in early ontogenesis of the capability for lateralization of this skill in the adult state in rats.

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Performance of juvenile mice in a reach-to-grasp task

Cited by 7 publications

References 17 publications

The neostriatum: two entities, one structure?

The neostriatum: two entities, one structure?

Optochemogenetic Stimulation of Transplanted iPS-NPCs Enhances Neuronal Repair and Functional Recovery after Ischemic Stroke

Formation of lateralization of manipulatory food-procuring behavior in the white rat early ontogenesis

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