Evaluation of Estrogen Receptor β Binding of Pruni Cortex and Its Constituents

Abstract

The bark of Prunus jamasakura Siebold (Pruni Cortex) has long been used in Japan as a folk remedy and is one of ingredients of the Kampo formula, Jumi-haidoku-to (JHT). JHT is used for treatment of skin diseases such as acne (acne vulgaris). According to Kampo medicinal sources, Quercus Cortex can be used in place of Pruni Cortex. In this study, we found that water extracts of Pruni Cortex, not Quercus Cortex showed a binding eŠect on estrogen receptor b (ERb). Thus, their chemical analysis was carried out by GC-MS and found thatˆve unique constituents (i.e., sakuranetin, naringenin, genistein, genkwanin and arctigenin) were detected in Pruni Cortex. The ERb binding capacity of these constituents was examined using 70 ng/ml 17b-estradiol, as the positive control with 100％ ERb binding. Among them, genistein (60％ at 10 ng/ml) showed the strongest binding capacity than naringenin (60％ at 1000 ng/ml) and sakuranetin (40％ at 1000 ng/ml). These results suggested that Pruni Cortex in JHT could play an important role in treatment of acne. In addition, those of Pruni Cortex from diŠerent harvest places were also examined in their chemical contents and ERb binding capacity. The extracts of Pruni Cortex from Kyushu in Japan and Anhui Province in China showed higher contents of genistein and stronger ERb binding capacity than that of Pruni Cortex from Tokushima Prefecture in Japan. Key words-Pruni

Pruni Cortex Extract Accelerates Wound Healing in a Mouse Model of Atopic Dermatitis

“…The stimulatory effect of PC on estrogen secretion may be related to this increase in the number of fibroblasts, since estrogen is known to activate fibroblasts through the estrogen receptor found on fibroblasts. It has been reported that PC binds to estrogen receptor β in vitro and increases estrogen secretion from skin fibroblasts [1]. At 4 weeks after AD induction, which is the chronic phase of AD, the PC-treated mice showed a decrease in the number of inflammatory cells in the dermis and only a few fibroblasts were seen.…”

“…Pruni Cortex (PC) is obtained from Prunus jamasakura or other related plants in the family Rosaceae as a crude drug extracted from bark without the periderm [1]. Since ancient times, it has been used widely in Japanese folk medicine, including for detoxification, the treatment of eczema, and as an antitussive [2].…”

“…It is also included in Jumihaidokuto, which is a Chinese herbal medicine that has been applied for the treatment of various skin diseases including acne vulgaris and atopic dermatitis (AD) [3]. The pharmacological actions of PC include antibacterial and antioxidant activities, the stimulation of estrogen secretion, and the inhibition of sebum synthesis and neutrophil activity; therefore, PC is expected to have various indications for skin diseases [1,[4][5][6][7].…”

Effect of Grapefruit Intake on Postprandial Plasma Glucose

“…Naringenin is repor ted to have a similar molecular structure to estrogen and to bind to estrogen β receptor 29) . When orchidectomized rats were fed GF, urinal deoxypridinoline, which is increased by orchidectomy, decreased to the original level 30) .…”

012 Jumihaidokuto (Shi-Wei-Ba-Du-Tang), a Kampo Formula, decreases the disease activity of palmoplantar pustulosis

“…Kampo medicines, such as orengedokuto (Huang-Lian-Jie-Du-Tang), unseiin (WenQing-Yin), and keishibukuryogan (Gui-Zhi-Fu-Ling-Wan) have been reported to be effective for PPP [2]. Jumihaidokuto (JHT, Shi-Wei-Ba-Du-Tang in Chinese) is a traditional herbal medicine that is composed of ten medicinal plants (Kikyo (Platycodi Radix), Saiko (Bupleuri Radix), Senkyu (Chuanxiong Rhizoma), Bukuryo (Sclerotium Poriae Cocos), Dokkatsu (Angelicae pubescentis Radix), Bofu (Saposhnikoviae Radix), Kanzo (Glycyrrhizae Radix), Keigai (Schizonepetae Herba), Shokyo (Zingiberis Rhizoma), and Bokusoku (Quercus cortex)) [3]. According to Kampo medicinal sources, Bokusoku (Quercus cortex) can be used in place of Ouhi (Pruni Cortex).…”