Evaluation of Epstein-Barr virus antigen-based immunoassays for serological diagnosis of nasopharyngeal carcinoma

Tang, Julian W.; Rohwäder, Edda; Chu, Ida Miu-Ting; Tsang, Raymond K. Y.; Steinhagen, Katja; Yeung, Albert T.; To, Ka‐Fai; Chan, Paul K.S.

doi:10.1016/j.jcv.2007.09.006

Cited by 24 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection of antibodies against HP-NAP in serum by ELISA Recombinant HP-NAP was prepared and purified, and ELISA was carried out as previously described [24][25][26][27] . Briefly, immunoplates (Nunc, Denmark) were coated with rNAP (5µg/well) and incubated overnight at 4℃.…”

Section: Screening For Seropositive Individuals With Igg Antibodies Amentioning

confidence: 99%

See 1 more Smart Citation

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Long¹,

Luo²,

Li³

et al. 2009

WJG

View full text Add to dashboard Cite

AIM: To detect and evaluate the antibodies againstHelicobacter pylori (H pylori ) neutrophil-activating protein (HP-NAP) in patients with gastric cancer and other gastroduodenal diseases. METHODS:Recombinant HP-NAP was prepared from a prokaryotic expression system in Escherichia coli . Serum positivity and level of HP-NAP-specific antibodies in sera from 43 patients with gastric cancer, 28 with chronic gastritis, 28 with peptic ulcer, and 89 healthy controls were measured by rHP-NAP-based ELISA. rHP-NAP-stimulated production of interleukin-8 (IL-8) and growth-related oncogene (GROα) cytokines in the culture supernatant of SGC7901 gastric epithelial cells was also detected. RESULTS:The serum positivity and mean absorbance value of HP-NAP-specific antibodies in the gastric c a n c e r g r o u p ( 9 7.7 % a n d 1 . 0 1 ± 0 . 2 4 ) w e r e significantly higher than those in the chronic gastritis group (85.7% and 0.89 ± 0.14, P < 0.005) and healthy control group (27.7% and 0.65 ± 0.18, P < 0.001). The sensitivity and specificity of ELISA for the detection of HP-NAP-specific antibodies were 95.5% and 91.5%, respectively. HP-NAP could slightly upregulate IL-8 production in gastric epithelial cell lines but had no effect on GROα production. CONCLUSION:Infection with virulent H pylori strains secreting HP-NAP is associated with severe gastroduodenal diseases, and HP-NAP may play a role in the development of gastric carcinoma. rHP-NAPbased ELISA can be used as a new method to detect H pylori infection. The direct effect of HP-NAP on gastric epithelial cells may be limited, but HP-NAP may contribute to inflammatory response or carcinogenesis by activating neutrophils.

show abstract

Section: Screening For Seropositive Individuals With Igg Antibodies Amentioning

confidence: 99%

“…A receiver operating cur ve (ROC) was plotted to calculate the cut-off values for antibodies against HP-NAP at a 95% accuracy level [26] . In addition, the cut-off values were determined by mean plus 2 × SD and mean plus 3 × SD, derived from P values in healthy individuals.…”

Section: Selection Of Cut-off Values For Antibodies Against Hp-nap Inmentioning

confidence: 99%

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Long¹,

Luo²,

Li³

et al. 2009

WJG

View full text Add to dashboard Cite

show abstract

“…Combined detection of anti-NA1, anti-early antigen (EA), anti-viral capsid antigen and anti-replication and transcription activator increases the sensitivity and has been considered as a useful serological method for NPC screening and diagnosis (16)(17)(18)(19)(20). Salivary IgA antibody titers were previously reported to be increased in response to EA, gp340 or NA1-p107 in healthy individuals compared with patients with NPC.…”

Section: Discussionmentioning

confidence: 99%

Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma

Jiang

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. Nuclear antigen-1 (NA1) protein of Epstein-Barr virus (EBV) is expressed in EBV-infected cells in the microenvironment of cancer. Since immune cells infiltrate abundantly in nasopharyngeal carcinoma (NPC) tumor tissues, we hypothesized that the local tumor microenvironment may perform an important role in the production of antibodies directed at NA1. Furthermore, we hypothesized that anti-NA1 antibody originating in the local microenvironment could be secreted into the saliva of patients with NPC. In the present study, 20 healthy controls and 39 patients with NPC treated with intensity-modulated radiation therapy were recruited for the study. Saliva and serum samples were collected from the NPC patients, and nasopharyngeal tissue samples from the patients with NPC. The titers of anti-NA1 antibody [immunoglobulin A (IgA)] were determined by ELISA. Expression of NA1, human leukocyte antigen-antigen D related (HLA-DR), cluster of differentiation (CD)80, CD86, CD3, CD4, CD19 and IgA was detected by immunohistochemical staining on paraffin-embedded nasopharyngeal tissue sections. Anti-NA1 antibodies were detected in the serum and saliva samples of the patients with NPC. In infiltrating cells, expression of HLA-DR, CD80, CD86, CD3, CD4, CD19 and IgA was detected, indicating that dendritic cells, T lymphocytes and B lymphocytes were all present in the local tumor tissues. Furthermore, expression of EBNA1 protein was detected on the membrane of the NPC tumor cells. Therefore, the NPC tumor microenvironment has the potential to initiate a humoral response to EBNA1 by producing IgA antibodies.

show abstract

“…An ELISA was performed according to previously published documents using prepared and purified recombinant HP-NapA31323334. Briefly, the purified NapA protein was diluted to 4 μg/ml and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Serum Helicobacter pylori NapA antibody as a potential biomarker for gastric cancer

Liu

Zhang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) infection is strongly associated with gastric cancer. However, only a minority of infected individuals ever develop gastric cancer. This risk stratification may be in part due to differences among strains. The relationship between neutrophil-activating protein (NapA) and gastric cancer is unclear. The purpose of this study is to evaluate the significance of NapA as a biomarker in gastric cancer. We used enzyme linked immunosorbent assay (ELISA) to determine the status of H. pylori infection. Indirect ELISA method was used for detection of NapA antibody titer in the serum of H. pylori infected individuals. Unconditional logistic regressions were adopted to analyze the variables and determine the association of NapA and gastric cancer. The results of study indicated serum H. pylori NapA antibody level were associated with a reduced risk for development of gastric cancer. It may be used in conjugation with other indicators for gastric cancer detection.

show abstract

Evaluation of Epstein-Barr virus antigen-based immunoassays for serological diagnosis of nasopharyngeal carcinoma

Cited by 24 publications

References 9 publications

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma

Serum Helicobacter pylori NapA antibody as a potential biomarker for gastric cancer

Contact Info

Product

Resources

About