Evaluation of entomopathogenic Bacillus sphaericus isolated from Lombok beach area against mosquito larvae

Suryadi, Bambang; Yanuwiadi, Bagyo; Ardyati, Tri; Suharjono, Suharjono

doi:10.1016/j.apjtb.2015.10.013

Cited by 13 publications

(9 citation statements)

References 15 publications

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“…Recent research has determined that 4 out of 20 isolates from B. sphaericus were moderately toxic to larvae of the mosquito pathogen (Suryadi et al, 2016). We believe the use of heat shock proteins from two reference species that are mosquito pathogens used in our study will be a new area of use in biological intervention.…”

Section: Discussionmentioning

confidence: 95%

Determination of heat shock proteins in certain Bacillus species

KONUK

2022

Frontiers in Life Sciences and Related Technologies

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This study investigated the effects of four different temperatures on the sporulation and development of 6 Bacillus species, 2 native and 4 reference. The SDS PAGE analysis showed that two different proteins, 40 and 39 kilodaltons, were produced by B. sphaericus ATCC 2362 after 24 and 48 hours at 48 °C, by local isolate 4 after 24 and 48 hours at 42 °C and by local isolate 31 after 24 and 48 hours at 48 °C. Additionally B. firmus ATCC 14573 produced a 40 kDa protein after 48 hours at 52 °C and B. thuringiensis var. israelensis produced a 42 kDA protein after 48 hours at 42 °C. At temperatures of 48 and 52 °C, after 12, 24 and 48 hours incubation, vegetative and heat resistant spore counts were determined to reduce by 104-106 fold according to bacterial counts. In conclusion, the data obtained in this study showed that at 48 and 52 °C spore vitality fell by a significant degree. Additionally the SDS PAGE analysis results revealed different heat shock proteins providing high temperature resistance with a 40 kDA protein produced by B. firmus ATCC 14573, 40-39 kDa proteins produced by B. sphaericus ATCC 2362, and local isolates 4 and 31 and 42kDA protein produced by B. thuringiensis var. israelensis.

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Section: Discussionmentioning

confidence: 95%

Determination of heat shock proteins in certain Bacillus species

KONUK

2022

Frontiers in Life Sciences and Related Technologies

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“…However soil samples have shown greater richness in the diversity of Bacillus species and related genera compared to other sources making it the most preferred substrate for isolation (González et al, 2013;Lobo et al, 2017;Suryadi et al, 2016). In this study, 453 Bacillus strains and related genera were isolated from 100 soil samples collected from random Strain PCR results 16s rRNA binA binB mtx1 mtx2 mtx3 cry48A cry49A cry4A cry11A cyt1A Ls C3-41 Positive; -, Negative. sites in Kenya.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and evaluation of mosquito entomopathogenic Bacillus species and related genera from randomly selected sites in Kenya

Karungu¹,

Huang²,

Atoni³

et al. 2018

Afr. J. Microbiol. Res.

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This study was carried out to identify specific mosquitocidal Bacillus species and related genera for future development of biopesticides in local mosquito control program in Kenya. Bacterial isolation was conducted from 100 soil samples through pasteurization method and preliminary identification conducted through phenotypical analysis. Toxicity analysis was performed through bioassays and lethal concentrations (LC) were determined using probit analysis. Toxic isolates were further identified through analysis of the 16s rRNA and screening of toxin genes through PCR. Expression of toxin proteins was performed using SDS-PAGE. Out of 453 isolates, 7 of them were found to yield highly potent toxicity (>50% mortality) against Culex quinquefasciatus during the initial toxicity assays. Among them, two isolates KDHa3 and SKDHb5, with LC50 values of 0.007mg/L and 0.008mg/L, respectively, were the most toxic against the target. Phylogenetic analysis based on 16s rRNA showed high homology to Lysinibacillus sphaericus (six isolates) and Bacillus thuringiensis (one isolate). Various toxin genes encoding BinA, BinB, Mtx (1, 2 and 3), Cry48A, Cry49A, Cry4A, Cry11A and Cyt1A were detected among the isolates. The protein profiles using SDS-PAGE were consistent with the standard strains Lysinibacillus sphaericus C3-41 and B. thuringiensis var. israelensis. Native toxic Bacillus species and related genera were identified with this study being the first to report highly toxic strains of L. sphaericus and B. thuringiensis strains from Kenyan soil samples.

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“…Since importing strain-based biopesticide/bacterial agents from foreign countries is an expensive and timeconsuming process, development of these agents on the local scale and promoting local industrial capability is very important (Suryadi et al 2016). Yadav et al improved a production system with medium formulation to breed potential bacterial agents using local and low-cost ingredients (2011).…”

Section: Discussionmentioning

confidence: 99%

Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi

Iskender¹,

Örtücü²,

Aksu³

2017

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi

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The fall webworm (Hyphantria cunea) is a polyphagous pest with numerous host plants. In the present study, the bacterial flora of H.cunea was investigated to identify new organisms that can be used as microbial control agent against the pest. Six bacteria were isolated and cultured from H. cunea. Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits) of bacterial isolates were determined. In addition, 16S rRNA gene region was sequenced. As a result of the studies conducted, bacterial isolates were identified as Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) and Microbacterium oxydans (Abk8). All bacterial isolates were tested for 12 days against third-fourth instar larvae of H. cunea. The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p<0.05). These results indicate that L. sphaericus (Abk1) can be taken into account in the microbial pest control of H. cunea. In the future, further studies will be conducted by using pathogenicity enrichment strategies of L. sphaericus (Abk1) (ex. combining with other entomopathogens or insecticides) in order to increase the effectiveness on H. cunea. Özet

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Evaluation of entomopathogenic Bacillus sphaericus isolated from Lombok beach area against mosquito larvae

Cited by 13 publications

References 15 publications

Determination of heat shock proteins in certain Bacillus species

Determination of heat shock proteins in certain Bacillus species

Isolation, identification and evaluation of mosquito entomopathogenic Bacillus species and related genera from randomly selected sites in Kenya

Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi

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