Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation

Genetic differences by platelet-specific antigens used for monitoring allomyelotransplant engraftment

“…In addition, there is a lag between recovery of hemopoiesis evaluated by peripheral blood erythrocyte markers and HSC engraftment in the bone marrow determined at the molecular level by genes encoding glycosyltransferase synthesis. Hemopoiesis recovery in the bone marrow anticipates the emergence of donor phenotype erythrocytes in the peripheral blood by 1-3 months [21].…”

Massive Immune Hemolysis After Non-myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation with Minor ABO-incompatibility

“…On day þ 21, chimerism analysis using multiplex PCR amplification of short tandem repeat-makers demonstrated that peripheral blood mononuclear cells and neutrophils were predominately the donor type (over 90%). In addition, ABO genotype of burst forming-unit erythroid from peripheral blood showed O/O, indicating that hemopoietic progenitors were the donor type [10]. On day þ 32, high fever and grade I GVHD in the skin occurred.…”

Persistent Expression of Recipient-type ABH Antigen after ABO-incompatible Allogeneic Hematopoietic Stem Cell Transplantation

“…In the setting of allogeneic hematopoietic stem cell transplantation (HSCT), RBCs are produced by donor-derived hematopoietic stem cells after HSCT, which express carbohydrates including sugars based on the donor's ABO gene. Therefore, in general, the recipient's ABO blood type ultimately changes to the donor's ABO blood type after ABO-incompatible HSCT (1)(2)(3). However, we experienced a 26-year-old male patient with acute myelogenous leukemia (AML) who underwent ABO-incompatible HSCT twice and persistently showed his original blood type even with AML in complete remission (CR) and proven complete donor-type chimerism.…”