Evaluation of Efficacy of Fungicides Against Fusarium oxysporum f. sp. lentis in Vitro at Lamjung, Nepal

Abstract

Fusarium is a soil borne pathogen which is common in almost all types of soil causing heavy losses in the crop production. Long term survival of pathogen in the soil as chlamydospores has increased its threat making it a highly devastating disease. High accessibility and simple application process of chemical control method has made it an effective and highly adopted approach of eliminating disease causing organisms. The present study was conducted in IAAS, Lamjung Campus to test the efficacy of fungicides in-vitro by poisoned food technique in PDA medium against Fusarium oxysporum f. sp. lentis. The fungicides tested were Carbendazim (50% WP), Chlorothalonil (75% WP) and Dithane M-45 (75% WP) at three different concentrations (100 ppm, 150 ppm and 200 ppm). The treatments were arranged in complete randomized design and replicated five times. The measurement of diameter of the fungal mycelium was taken 8 times at 48 hours interval until the fungus nearly covered the plate in control treatment and inhibition percent of the chemicals were calculated. All the fungicides inhibited the fungal growth significantly, among which carbendazim was highly effective in all the concentrations reducing 100% of mycelial growth followed by chlorothalonil. Dithane M-45 showed least inhibition i.e. 26.62% in 200 ppm (day 13). The chemicals exhibited increased tendency of inhibition with increased concentration.

Saccharothrix Algeriensis NRRL B-24137 Potentiates Chemical Fungicide Carbendazim in Treating Fusarium Oxysporum f.sp. Vasinfectum-Induced Cotton Wilt Disease

“…It was observed that inhibition of the pathogen growth also increased with increasing concentration of all fungicides which are in complete agreement with those of Dahal et al 41 In the current research, carbendazim was found to be the most potent chemical in all concentration against the wilt pathogen as it almost totally inhibited the fungus mycelium growth. This result was in congruent with the findings of Patil et al, 42 Maheshwari et al, 43 Srivastava et al 44 and Dahal et al 41 Khanzada et al 45 and Narayanan et al 46 who found complete inhibition of F. oxysporum by carbendazim. Rajput et al 27 reported best result with carbendazim to reduce the mycelial growth of F. oxysporum f. sp.…”

“…There was a positive association between concentration and inhibition of the pathogen growth. It was observed that inhibition of the pathogen growth also increased with increasing concentration of all fungicides which are in complete agreement with those of Dahal et al 41 …”

Pathogenic Variability and Fungicidal Sensitivity of Fusarium spp. Causing Wilt in Chilli in Karnataka

“…Variations in sensitivity against fungicides in different Fusarium spp. and isolates have also been reported by Rajput et al, 2006;Amini et al, 2010;Andrabi et al, 2011;Maitlo et al, 2014;Dahal and Shrestha 2018.…”

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

“…To maintain the level of faba bean yield both quantitatively and qualitatively, farmers often rely heavily on the use of chemical fungicides to control the disease [ 11 ]. Conversely, the application of chemical fungicides in faba bean growing field results in the development of resistance by the plant pathogens, hazardous to human health, pollute the environment and harm biodiversity of the ecosystem [ 12 ].…”