In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

Abstract

Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

Efficacy of the biocontrol agent Trichoderma hamatum against Lasiodiplodia theobromae on macadamia

“… Padder and Sharma (2011) reported that culture filtrate of T. hamatum had a significant inhibitory effect on spore germination of Colletotrichum lindemuthianum , and the inhibitory activity of culture filtrates of Trichoderma sp. have also been reported against other fungal pathogens, including Fusarium solani ( Dugassa et al, 2021 ) and F. oxysporum ( Shanmugam et al, 2008 ). In the current study, the cell-free culture filtrate of T. hamatum at a concentration ranging from 0.5 to 10% markedly inhibited the mycelial growth of L. theobromae , with inhibitory activity increasing with concentration.…”

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

“…Several authors have suggested that combinations of introduced microbial agents have to be compatible with each other for better and more consistent disease suppression ( Chien and Huang, 2020 ; Dugassa et al, 2021 ). Bacillus species, particularly B. amyloliquefaciens and B. subtilis , can produce a wide range of antifungal compounds such as lipopeptide antibiotics (e.g., iturin, surfactin, and fengycin) and lytic enzymes (e.g., catalase, protease, and cellulase) ( Xiong et al, 2015 ).…”

Harnessing Trichoderma in Agriculture for Productivity and Sustainability

“…This was shown by the increase of Archaeorhizomyces and Trichoderma while decreasing Ophiosphaerella abundance. In one in vitro study, Trichoderma was seen to inhibit the growth of Fusarium solani, but no inhibition area was observed when it was cultured together with Pseudomonas strains [105]. Trichoderma tends to be compatible and live mutually with beneficial microbes that contribute to plant growth rather than harmful ones.…”