Evaluation of effect of four types of fissure sealant on growth inhibition of streptococcusmutans and lactobacillus acidophilus )In vitro(

Abstract

Background and Aim: Since some sealants can control cariogenic microorganisms, therefore the evaluation of antibacterial effect of different sealants are the subject of this study. Materials and Methods: in this experimental study, the plates were divided into 5 groups.Group I control:two plates including Blood Agar with Mutans Streptococcus (SM) & the other MRS with Lactobacillus Acidophilus (LA).Group II:6 plates Blood Agar culture with two wells and inoculation of SM with addition of two fissure sealants; Clinpro 3M with fluoride &flowable composite 3M without fluoride.In group III all the steps were the same as group IIexcept that in which fissure sealants used were Conseal F with flouride& Eco-s without fluoride.In groups IV & V all the steps were the same as groups II & III respectively except that MRS culture media with inoculation of Lactobacillus Acidophilus was used.The diameter of the growth inhibition halo (zone of inhibition) was measured using calipers. The experiment was repeated for three consecutive days with 26 plates and the results were recorded every 48 hours.The data were analyzed using Two-way ANOVA Results: The diameter of growth inhibition halo for Clinpro 3M in the medium where Streptococcus mutans was cultured was 27/29+ 3/59mm&where Lactobacillus Acidophilus was cultured was 12/37+ 1/46mm which differed from other three materials significantly. (p<0.000) Conclution: It seems, Clinpro 3M had the most inhibitory effect on Streptococcus mutans and , Lactobacillus acidophilus growth.