Evaluation of early changes of cartilage biomarkers following arthroscopic meniscectomy in young Egyptian adults

Koryem, Hamdy Khamis; Wanas, Mohamed Adel Abd El Qawy; Rizk, Mohamed M.; Kotb, Hesham Taha; Naguib, Abir; Shafei, Mohamed Mahmoud Abdel Hamid El; Naby, Hoda Mohammed Aly Abd El

doi:10.1016/j.ajme.2014.06.005

Cited by 3 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meniscus damage and loss results in both local biological and mechanical responses in the meniscus and joint tissues (16–19), which may also be reflected systemically. These biological changes can be quantified by measuring biomarkers that reflect the processes of extracellular matrix (ECM) synthesis and degradation and inflammation, presumably reflecting joint remodeling and disease progression (16, 20).…”

Section: Introductionmentioning

confidence: 99%

“…These biological changes can be quantified by measuring biomarkers that reflect the processes of extracellular matrix (ECM) synthesis and degradation and inflammation, presumably reflecting joint remodeling and disease progression (16, 20). Therefore, a further understanding of biomarker profiles and their relationship to joint injury may identify patients at risk for meniscus injury and PTOA following meniscus injury.…”

Section: Introductionmentioning

confidence: 99%

“…In order to identify candidate biomarkers relevant to joint injury and potentially OA progression, synovial fluid, serum, and urine samples from healthy individuals and OA patients have been evaluated for biomarkers of both joint tissue metabolism and inflammation (21–28). While many of these biomarkers have been researched in subjects with anterior cruciate ligament (ACL) tears and intra-articular fractures (28–35), limited biomarker data are available on meniscus tear subjects (16, 18, 21). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Matrix metalloproteinase activity and prostaglandin E2 are elevated in the synovial fluid of meniscus tear patients

Liu

Goode

Carter

et al. 2017

Connective Tissue Research

View full text Add to dashboard Cite

Purpose Meniscus tears are a common knee injury and are associated with the development of post-traumatic osteoarthritis (OA). The purpose of this study was to evaluate potential OA mediators in the synovial fluid and serum of meniscus tear subjects compared to those in the synovial fluid of radiographic non-OA control knees. Materials and Methods Sixteen subjects with an isolated unilateral meniscus injury and six subjects who served as reference controls (knee Kellgren-Lawrence grade 0–1) were recruited. Twenty-one biomarkers were measured in serum from meniscus tear subjects and in synovial fluid from both groups. Meniscus tear subjects were further stratified by tear type to assess differences in biomarker levels. Results Synovial fluid total matrix metalloproteinase (MMP) activity and prostaglandin E2 (PGE2) were increased 25-fold and 290-fold, respectively, in meniscus tear subjects as compared to reference controls (p<0.05). Synovial fluid MMP activity and PGE2 concentrations were positively correlated in meniscus tear subjects (R=0.83, p<0.0001). In meniscus tear subjects, synovial fluid levels of MMP activity, MMP-2, MMP-3, sGAG, COMP, IL-6, and PGE2 were higher than serum levels (p<0.05). Subjects with complex meniscus tears had higher synovial fluid MMP-10 (p<0.05) and reduced serum TNFα and IL-8 (p<0.05) compared to other tear types. Conclusions Given the degradative and pro-inflammatory roles of MMP activity and PGE2, these molecules may alter the biochemical environment of the joint. Our findings suggest that modulation of PGE2 signaling, MMP activity, or both following a meniscus injury may be targets to promote meniscus repair and prevent OA development.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Matrix metalloproteinase activity and prostaglandin E2 are elevated in the synovial fluid of meniscus tear patients

Liu

Goode

Carter

et al. 2017

Connective Tissue Research

View full text Add to dashboard Cite

show abstract

“…In young subjects, intact meniscal tissue may be ruptured by trauma; meniscal tearing often induces fluid accumulation, perceived as swelling and a sense of knee fullness. This type of damage triggers local biological and mechanical responses in the meniscus and joint tissues [20,29]. A normal meniscus may suffer acute tearing creating trauma, or degenerative tearing after abnormal meniscal loading.…”

Section: Introductionmentioning

confidence: 99%

“…Meniscal tearing is a major risk factor for the development of post-traumatic arthritis and increases, by 50%, the probability of symptomatic osteoarthritis (OA) within 10-20 years of post-injury repair [26]. Such biological changes can be quantified by measuring biomarkers reflecting extracellular matrix (ECM) synthesis and degradation, and inflammation, presumably acting as surrogates of joint remodelling and disease progression [20,27]. The balance between matrix metalloproteinase (MMP)-mediated matrix synthesis and inhibition is crucial in terms of meniscal repair [7].…”

Section: Introductionmentioning

confidence: 99%

Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears

Atiç

Deveci

2019

Folia Morphol

View full text Add to dashboard Cite

Background: A meniscus tear is a serious trauma that develops during swinging motion of the fixed foot. Meniscus tears may also be accompanied by divergence of the lateral ligaments of the knee joint. Materials and methods: We enrolled 45 males and 35 females with meniscal tears in the present study. Patients with local joint pain, swelling, difficulty climbing stairs, patellar creeping, difficulties with daily living activities, local pain on palpation, and walking and running complaints, were included. We performed preoperative magnetic resonance imaging. Synovial fluid (5 mL) was aspirated from the lateral suprapatellar pouch of each knee with meniscal pain with the patient in the supine position. Blood samples were taken and biochemical parameters were analysed. The Harris haematoxylin and eosin staining protocol was used to evaluate tissue samples, and the levels of anti-matrix metalloproteinase (MMP)-9 and anti-tumour necrosis factor alpha (TNF-α) antibodies were measured immunohistochemically. Results: Increased numbers of lymphocytes and neutrophils, hyperplastic erythrocytes, and fibroblasts were observed in the joint fluid of females. In males, the fibroblast cells were hyperplastic and plasma cell numbers were increased. MMP-9 expression was elevated in plasma cells, fibroblasts, and neutrophils; and TNF-α expression was observed in lymphocytes and polymorphic nucleated cells. We suggest that increased fluid levels in inflamed joints with meniscal tears, and the associated inflammation, disrupt the cartilage matrix and elevate the production of cytokines such as TNF-α and MMP-9 via release from cells such as fibroblasts that synthesise these mediators. Conclusions: Anti-TNF-α treatment may prevent meniscal tears and prevent or slow the development of osteoarthritis.

show abstract

Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu

Baloğlu

Özkorkmaz

2019

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi

View full text Add to dashboard Cite

Menisküs zorlanmalara karşı emici özelliği ile eklem stabilitesini sağlar. Menisküs yırtığı ise yaşam kalitesini düşüren ciddi bir travmadır. Bu çalışmada, menisküs yırtığı durumunda sinoviyal sıvıdaki hücrelerde meydana gelen farklılıkların immünohistokimyasal yöntemlerle incelenmesi amaçlanmıştır. Gereç ve Yöntem: Menisküs yırtığı olan 28 erkek ve 32 kadın hasta çalışmaya dahil edilmiştir. Hastaların fizik muayenede lokal ağrı hissi, şişme, günlük aktivitelerini yerine getirmekte ve koşma-yürümede güçlük gibi şikayetleri olduğu belirlendi. Hastaların manyetik rezonans görüntülemeleri (MRG) değerlendirildi. Menisküs ağrısı olan dizlerin yanal suprapatellar poş kısmından enjektör ile 5 cc sinoviyal sıvıları aspire edildi. Örneklerin rutin histolojik takipleri yapıldıktan sonra parafine gömüldü ve mikrotom ile yarı-ince kesitleri alındı. İmmunohistokimyasal analiz için matriks metaloprotein (MMP-2) ve nükleer faktör kappa (NF-kβ) primer antikorları kullanıldı. Kesitlerin şık mikroskobu ile fotoğrafları çekildi ve değerlendirildi. Bulgular: MRG sonucu eklem içerisinde mayi artışı ve parçalı yırtık izlendi. Kadın hastaların eklem sıvılarında lenfosit ve nötrofil hücrelerinde ve hiperplastik eritrositler ile fibroblast sayısında artış gözlendi. Erkek hastalarda, fibroblast hücreleri hiperplastik olarak izlendi ve plazma hücrelerinin arttığı görüldü. MMP-2 ekspresyonunun plazma hücrelerinde, fibroblastlarda ve nötrofillerde arttığı ayrıca, NF-kβ ekspresyonunun lenfosit ve polimorfik çekirdeğe sahip hücrelerde pozitif olduğu izlendi. Sonuçlar: Menisküs yırtığı ve enflamasyonu olan eklemlerde artmış sıvının kıkırdak matriksinde hasara neden olduğu ve buna bağlı olarak MMP-2 ve NF-kβ gibi sitokinlerin ekspresyonunun arttığı, fibroblast hücreleri gibi sentez yapan hücrelerin yıkıma uğradığı düşünülmektedir.

show abstract

Evaluation of early changes of cartilage biomarkers following arthroscopic meniscectomy in young Egyptian adults

Cited by 3 publications

References 23 publications

Matrix metalloproteinase activity and prostaglandin E2 are elevated in the synovial fluid of meniscus tear patients

Matrix metalloproteinase activity and prostaglandin E2 are elevated in the synovial fluid of meniscus tear patients

Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears

Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu

Contact Info

Product

Resources

About