Evaluation of drug-resistant Enterobacteriaceae in retail poultry and beef

Kilonzo‐Nthenge, Agnes; Rotich, Emily; Nahashon, Samuel N.

doi:10.3382/ps.2012-02581

Cited by 49 publications

(37 citation statements)

References 57 publications

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“…They could emerge as foodborne pathogens as they were previously isolated from outbreaks. An earlier study reported the isolation of multidrug-resistant Morganella morganii in US retail poultry and beef [37], and Serratia spp. (14.3%) were among the most prevalent contaminants.…”

Section: Discussionmentioning

confidence: 99%

Foodborne bacterial pathogens recovered from contaminated shawarma meat in northern Jordan

Nimri

Al-Dahab

Batchoun

2014

J Infect Dev Ctries

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Introduction: The purpose of the study was to isolate, identify, and determine the antimicrobial resistance of the bacterial pathogens recovered from shawarma (donair) sandwiches served to the public in Jordan. Methodology: Bacterial contamination of 100 shawarma sandwiches with pathogenic bacteria was studied by culture on selective media, serology, PCR assay, and antimicrobial susceptibility testing. Results: One hundred and forty-five bacterial isolates were identified. The predominant species was Escherichia coli (28.3%), with six isolates of serotype O157:H7, followed by Salmonella spp. (25.5%). Higher contamination rates were found in chicken sandwiches. The majority of these bacteria expressed high resistance to several antimicrobials, especially tetracycline and streptomycin. Citrobacter freundii was isolated from 15.9% and Staphylococcus aureus was isolated from 8.3% of the sandwiches. The presence of these pathogens is of primary concern because some strains are capable of producing a heat-stable enterotoxin that causes food poisoning in humans, and should therefore be taken into account in risk assessment. Conclusions: Results signify the importance of sustained surveillance of foodborne pathogens in shawarma sandwiches to minimize the risk of contamination. Availability of data on the isolated pathogens and modes of transmission in food from different countries would provide a common ground for reaching international agreement on food safety regulations.

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Section: Discussionmentioning

confidence: 99%

Foodborne bacterial pathogens recovered from contaminated shawarma meat in northern Jordan

Nimri

Al-Dahab

Batchoun

2014

J Infect Dev Ctries

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“…There are a number of reports of antimicrobial resistance in bacteria found in meat products [28,29]. However, there are relatively fewer such reports on produce [30].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Resistance in Enterobacteriaceae and Coliforms Isolated on Farm, Packaged and Loose Vegetables in Kentucky

Topè¹,

Hitter²,

Patel³

2016

J Food Microbiol Saf Hyg

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Fresh produce normally carry epiphytic microorganisms; however, it can be contaminated with pathogenic bacteria. Categorized as 'ready-to-eat', most vegetables are consumed raw, thus, may present a food safety risk. Over the last three decades, global consumption of fresh vegetables has increased considerably, and the market has expanded by more than 20%. Concomitantly, the number of outbreaks involving fresh vegetables has increased significantly. Enterobacteriaceae members are involved in most of the bacterial outbreaks linked to fresh produce. There is a worldwide concern about the increased use of antimicrobials in agriculture. Antimicrobial resistant bacteria enter the food chain from the farm, often due to the use of animal manure. In the current study, the presence of entero-coliform bacteria and their antimicrobial susceptibilities in fresh vegetables sampled from small farms to retail was evaluated. Samples of vegetables were (i) collected directly from small farms from central Kentucky (n=59) and (ii) from four supermarkets in Frankfort, KY including loose and pre-packaged produce (n=72), analyzed for isolation of entero-coliform species. Members of the Enterobacteriaceae were detected on 25% of farm and 40% of retail produce, respectively. Approximately, 61% of the packaged produce and 19.4% of the loose produce had entero-bacterial presence, respectively. Their resistance to fourteen common antimicrobials was tested using Kirby-Bauer method. Approximately, 63% of isolates from farm and 70% of isolates from retail produce displayed resistance to at least three antimicrobial agents, while 18% of the isolates from farm and 41% from retail samples displayed resistance to at least ten antimicrobial agents. We conclude that 'ready-to-eat' fresh vegetables can be a source of exposure to pathogens with multiple drug resistance (MDR), defined as resistance to at-least three antimicrobial agents, leading to greater risks in immunocompromised individuals, and may serve as reservoirs for resistance gene transfers in human colon.

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“…Contamination of Enterobacteriaceae in chicken carcass came from contents of intestinal tract during evisceration 6 . Enterobacteriaceae is used as hygiene indicator in food industry.…”

Section: Discussionmentioning

confidence: 99%

“…Enterobacteriaceae is used as hygiene indicator in food industry. The study on contamination of Enterobacteriaceae in meat from retail stores in Tennessee, USA 6 found the contamination level of Enterobacteriaceae in raw meats ranged from 3.26 log10 cfu/g to 4.94 log10 cfu/g. The contamination level of Enterobacteriaceae in duck meat in this study was relatively same.…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial resistance is increasing in several species of Enterobacteriaceae and this become a major concern in human and animal health. The widespread use of antibiotics in livestock has implicated to emergence of antibiotic resistant bacteria 6 . Enterobacteriaceae showed antibiotic resistant mainly to beta lactam antibiotics including carbapanem, especially genera such as Escherichia, Klebsiella, Citrobacter, Serratia, Salmonella, Enterobacter, and Proteus 7 .…”

Section: Discussionmentioning

confidence: 99%

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Contamination of Antibiotic Resistant Enterobacteriaceae

Lukman¹,

Latif²,

Pisestyani³

et al. 2018

Proceedings of the 1st International Conference in One Health (ICOH 2017)

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This study was aimed to determine Enterobacteriaceae contamination that were resistant against aantibiotics in duck meat which was related with food safety for the consumers. Total of 52 samples of ducks were taken from 5 subdistricts in Bogor District, i.e., Ciomas, Gunung Sindur, Klapanunggal, Jasinga, and Parung Panjang. This sample size was calculated based on the assumption of 95% confidence level, 50%predicted prevalence, and 10% standard error. The ducks were collected from small-scale farmers which supplied duck meat to markets, so the ducks were slaughtered by them. The samples of duck meat were handled aseptically and transported in cool box to the laboratory. The total of Enterobacteriaceae was examined using plate count method with violet red bile glucose agar according to Kornacki and Johnson (2001). The examination of antibiotic resistance was done using disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) Guidelines (CLSI 2012). The data was analysed descriptively. The results showed that the total of Enterobacteriacea were 79679 ± 260463 cfu/g. The majority of Enterobacteriaceae isolated from duck meat (84.6%) showed resistant against 5 of antibiotic (gentamycin, kanamycin, tetracycline, trimethoprim, and ampicillin). The Enterobacteriaceae had resistance against tetracycline (93.2%), trimethoprim (88.6%), and ampicillin (81.8%). Nevertheless, this family was still sensitive againts kanamycin (34.1%) and gentamycin (15.9%). The isolated bacteria (69.2%) showed multi-antibiotic 251

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Evaluation of drug-resistant Enterobacteriaceae in retail poultry and beef

Cited by 49 publications

References 57 publications

Foodborne bacterial pathogens recovered from contaminated shawarma meat in northern Jordan

Foodborne bacterial pathogens recovered from contaminated shawarma meat in northern Jordan

Evaluation of Antimicrobial Resistance in Enterobacteriaceae and Coliforms Isolated on Farm, Packaged and Loose Vegetables in Kentucky

Contamination of Antibiotic Resistant Enterobacteriaceae

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