Evaluation of Dot-ELISA and enzyme-linked immuno-electrotransfer blot assays for detection of a urinary hydatid antigen in the diagnosis of cystic echinococcosis

Swarna, S. R.; Parija, Subhash Chandra

doi:10.4103/2229-5070.97238

Cited by 10 publications

(5 citation statements)

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“…In the current study, ELISA and IHA were used to diagnose CE in a sub-set of patients (n = 48). However, the usefulness of serology is limited due to poor sensitivity and the possibility of cross-reactions occurring with other parasitic infections (Chaya and Parija 2013;Dubey et al 2011;Schantz et al 1980;Swarna and Parija 2012;Wuestenberg et al 2014). Because of these reasons, patients are typically not diagnosed based on serology alone.…”

Section: Discussionmentioning

confidence: 99%

Surgically managed human cystic echinococcosis in north-eastern Iran: a single center’s experience from 2001 to 2008

et al. 2017

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Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the tapeworm . Although CE has a large geographic distribution, data are lacking on the frequency of infection and epidemiology of CE in many endemic areas of the world, including the Middle East. Demographic and clinical information on surgically managed human CE cases were evaluated from a referral hospital in north-eastern Iran for the years 2001-2008. Of the 400 CE cases, 218 (54.5%) were male. The median age of patients was 35 years (range 2-83 years). The lungs (41.0%) and liver (37.7%) were the most commonly infected organs. However, 12.7% of patients had multiple organ involvement. The majority of cases (54.3%) were diagnosed using ultrasound, with only 12.0% diagnosed with the help of serology. Total white blood cell count was elevated in 26.8% (107/400) of patients, neutrophil count was elevated in 24.0% (96/400) of patients, and eosinophil count was elevated in 13.3% (53/400) of patients. Lymphocyte count was the only complete blood count (CBC) value that differed based on organ location ( = 0.001). Despite some successes in the control of CE, the number of surgical CE cases in north-eastern Iran remains high. Although not diagnostic alone, CBC values allow for clinicians to obtain a more complete clinical picture of CE before, during, and after treatment. While serology has its place, the use of diagnostic imaging continues to be the most commonly used tool for the diagnosis of CE cases.

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Section: Discussionmentioning

confidence: 99%

Surgically managed human cystic echinococcosis in north-eastern Iran: a single center’s experience from 2001 to 2008

et al. 2017

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“…1 There exist diverse serological tests such as indirect hemagglutination, enzyme-linked immunosorbent assay (ELISA), latex agglutination test among others. 7 These tests are infrequently used due to their low sensitivity and low specificity with up to 30% false-negative and up to 25 % false positive respectively. 2 This was vindicated in a serological study of Hydatid disease where out of the suspected 40 cases for Hydatid disease of the pancreas, only 15 cases showed seropositive for CE.…”

Section: Introductionmentioning

confidence: 99%

The Contribution of Hydatid Serology in the Diagnosis and Monitoring of Hydatid Cyst in the Parasitology and Mycology Department at the Avicenna Military Hospital of Marrakesh, Morocco

Oussaga¹,

ET-TAHOURI²,

Lmimouni³

et al. 2022

IJMPS

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Introduction: Hydatid disease is also known as cystic echinococcosis (CE) is a zoonotic disease caused by a parasitic infection. CE is caused by the larval stage of Echinococcus granulosus. Humans are accidental intermediate hosts and they get infected following the accidental ingestion of Echinococcus granulosus eggs. Usually, the disease is diagnosed regarding clinical history, serologyand imaging. Aims: To evaluate the contribution of Hydatid serology in the diagnosis and monitoring of Hydatid cysts in the parasitology and mycology department at the AMH of Marrakesh, Morocco. Material and Methods: This study was conducted to evaluate the contribution of Hydatid serology in the diagnosis and monitoring of Hydatid cysts in the parasitology and mycology department at the Avicenna Military Hospital (AMH) of Marrakesh, Morocco. A retrospective study among 62 suspected cases from January 2018-December 2020 was conducted. Results: The ELISA test was used for serology. Out of the 62 cases, 23 (37%) were seropositive. Most of the seropositive cases had an average age of 38 years and were from rural areas, 86.5%. The liver was the most affected organ, 56.5%, and hypochondriac pain was the most prevalent symptom, 62.7%. Otherwise, the false-negative cases for the ELISA test were high implying the test had low sensitivity and specificity. Otherwise, the ELISA test can be used for the diagnosis and monitoring of Hydatid disease. Conclusion: Hydatid cyst is relatively common in Morocco with significant morbidity and mortality and timely detection is imperative towards management and preventive efforts.

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“…This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. logical tests (7,8). Serology-based methods have been innovated for the total screening of population in endemic areas but the sensitivity and specificity of the diagnostic antigens are of importance (9,10).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Hydatidosis in Kaboodarahang, Hamadan Province, Iran, in 2016 - 2017

Barati

Sharifi‐Sarasiabi

Hamedi

et al. 2018

Hormozgan medical journal

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Background: Cystic echinococcosis (CE) or hydatid cyst as a significant zoonotic disease caused by Echinococcus spp. is endemic in Iran. The present study was carried out to clarify the status of hydatidosis in Kaboodarahang, Hamadan province. Objectives: This descriptive cross-sectional study aimed to investigate the prevalence of the hydatid cyst seroepidemiology in Hamadan province, Iran. Hamadan province was chosen due to its wide range of livestock breeding and human exposure to stray dogs. We studied the seroprevalence of the parasite and infection risks. Methods: In this cross-sectional study, overall 1232 serum samples (524 from males and 708 from females) were collected by randomized cluster sampling in 2016-2017. The sera were analyzed by the enzyme-linked immunosorbent assay (ELISA) using IgG ELISA kits. Before taking the specimens, a questionnaire was filled out for each enrolled patient. The data were analyzed using the Chi-square test and multivariate logistic regression for risk factors analysis. Results: The seropositivity was 1% (12 cases including four males and eight females). No significant difference was seen according to gender (P = 0.51). There was no significant association between CE seropositivity and age group, occupation, and region, but the association between keeping a dog (in the house or workplace) and a positive IgG test was statistically significant (P = 0.02). Conclusions: Although the seroprevalence of CE was relatively low in Hamadan province, yet, due to the importance of the disease and zoonotic feature of the organism, all preventive measures should be taken into consideration.

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Evaluation of Dot-ELISA and enzyme-linked immuno-electrotransfer blot assays for detection of a urinary hydatid antigen in the diagnosis of cystic echinococcosis

Cited by 10 publications

References 8 publications

Surgically managed human cystic echinococcosis in north-eastern Iran: a single center’s experience from 2001 to 2008

Surgically managed human cystic echinococcosis in north-eastern Iran: a single center’s experience from 2001 to 2008

The Contribution of Hydatid Serology in the Diagnosis and Monitoring of Hydatid Cyst in the Parasitology and Mycology Department at the Avicenna Military Hospital of Marrakesh, Morocco

Seroprevalence of Hydatidosis in Kaboodarahang, Hamadan Province, Iran, in 2016 - 2017

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