Evaluation of Dose Dependent Chemopreventive Effect of Diosmin on Induced Oral Squamous Cell Carcinoma

Abstract

Purpose: This study aimed to evaluate the chemoprevention efficacy of diosmin on experimentally induced hamster buccal pouch (HBP) squamous cell carcinoma utilizing Ki 67 as a proliferative marker. Material and Methods: Seventy Syrian male hamsters were used as experimental animals, these hamsters were divided into five groups, Group I (negative control): 5 hamsters were left untreated. Group II (DMBA treated group): 20 hamsters were divided equally into two subgroups, painted with 0.5% DMBA where subgroup IIa: for 8 weeks while subgroup IIb: for 14 weeks. Group III: 5 hamsters were orally administrated diosmin 150 mg/kg for 14 weeks. Group IV: 20 hamsters were divided equally into two subgroups, treated with diosmin 100mg/kg and DMBA where subgroup IVa: for 8 weeks while subgroup IVb: for 14 weeks. Group V: 20 hamsters were divided equally into two subgroups, treated with diosmin 150mg/ kg and DMBA where subgroup Va: for 8 weeks while subgroup Vb: for 14 weeks.Results: Comparing all groups and subgroups revealed that the mean values have been arranged with the following descending pattern subgroup IIa, subgroup Va, subgroup IIb, subgroup Vb, subgroup IVb, subgroup IVa, group III and group I. ANOVA test revealed a statistically significant difference between groups. Conclusion: Diosmin 100mg/kg is considered a promising chemopreventive agent in preventing induced HBP squamous cell carcinoma.

Diosmin nanocrystal gel alleviates imiquimod-induced psoriasis in rats via modulating TLR7,8/NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu, and Tregs/Th17 balance

“…Additionally, our results revealed significant elevation in the expression of PCNA in the skin of untreated psoriatic rats, indicating an increased level of cell proliferation. Previous studies demonstrated the effect of diosmin of different proliferation markers in different types of cancers such as hepatocellular carcinoma (Tahir et al 2013 ), oral squamous cell carcinoma (Osman et al 2022 ), colon (Zeya et al 2022 ) and gastric cancer (Hu et al 2013 ). Moreover, AKT indirectly induces the transcription of anti-apoptotic genes such as BCL2 via NF-κB factors (Mercurio et al 2021 ; Ozes et al 1999 ; Kane et al 1999 ).…”

“…The current study is considered the first study to investigate the efficacy of different diosmin formulations to halt the immune pathogenesis and inflammatory environment of psoriasis. Previous studies reported the anti-inflammatory and immunomodulatory effect of diosmin on the skin keratinocytes in other dermatological diseases such as atopic dermatitis via modulation of Th2 cells activity and the inflammatory cytokines such as IL6 and IL1 (Osman et al 2022 ; Feldo et al 2022 ). In addition, Li et al recently showed that hesperidin, the citrus flavonoid that diosmin is derived from, has the ability to reduce the psoriatic like skin lesions in mice and inhibit the keratinocytes proliferation (Lee et al 2021 ).…”