Evaluation of DNA Methylation Changes by CRED–RA Analysis Following Prednisone Treatment of Endophyte, Fusarium oxysporum

Harshitha, K; Nair, R. Aswati

doi:10.1007/s12088-020-00857-8

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…A similar level of oxidative stress induction up to +1.5-fold increase in the IC 50 (half maximal inhibitory concentration) set was found in the Schizosaccharomyces pombe treated with the plant-derived essential oil compound Previous studies related to the determination of GTS values in regard with the specific treatment of chemical compounds on Fusarium spp. revealed GTS% of 66.27% to 90.9% (Gazdağlı et al, 2018;Yörük, 2018;Harshitha, Nair, 2020;Teker et al, 2021;Albayrak et al, 2023). The GTS value of 90.47% for the RH treatment appears to be a relatively lower level of genomic instability in the F. graminearum genome.…”

Section: Resultsmentioning

confidence: 95%

Rutin hydrate induces autophagic cell death and oxidative stress response in phytopathogenic fungus Fusarium graminearum

Yörük

2023

Zemdirbyste-Agriculture

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Fusarium graminearum infects cereal crops of high commercial and nutritional value such as barley, wheat, and maize, and causes various diseases. Due to the increasing world population and changing climatic conditions, the importance of controlling this pathogen is increasing. The aim of this study was to investigate the potential antifungal effect of the flavonoid compound rutin hydrate (RH) on the F. graminearum PH-1 reference strain at different levels. The EC 50 (half effective concentration) value was determined by the agar dilution method on the PDA (potato dextrose agar) medium containing different concentrations of rutin hydrate. To examine the effect of rutin hydrate on cell viability, the WST-1 toxicity test was performed. To determine the effect of rutin hydrate on genomic stability and methylation profiles, the CRED-RA (coupled restriction enzyme digestionrandom amplification) analysis was performed. To determine the expression levels of atg5, cat, kmet1, sporulation (mgv1), and apoptosis (mst20) related genes, the qRT-PCR (quantitative real-time polymerase chain reaction) was performed. To detect the presence of oxidative stress and autophagy, the DCF-DA (2',7'-dichlorofluorescein) and MDC (monodansylcadaverine) staining tests were used. The EC 50 value of rutin hydrate was determined as 580 µg mL −1 . The WST-1 analysis revealed that rutin hydrate caused cytotoxicity (58.53%) in F. graminearum (p < 0.05). In the CRED-RA analysis, genomic template stability (GTS) decreased by 9.53%, and the methylation levels for HapII and MspI enzymes were 11.6% and 5.24%, respectively. There was a significant difference in the atg5 (2.66 ± 0.48; p < 0.01), cat (3.00 ± 0.46; p < 0.01), and kmet1 (6.29 ± 2.24; p < 0.05) expression in the rutin hydrate-treated samples. Autophagy and oxidative stress response with a significant difference (p < 0.05) in the rutin hydrate-treated F. graminearum was confirmed by DCF-DA and MDC tests. This is the first report demonstrating the antifungal effect of rutin hydrate on F. graminearum at physiological, transcript, and epigenetic levels.

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Section: Resultsmentioning

confidence: 95%

Rutin hydrate induces autophagic cell death and oxidative stress response in phytopathogenic fungus Fusarium graminearum

Yörük

2023

Zemdirbyste-Agriculture

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“…Furthermore, the changes observed in the GTS rates highlight the significance of varying Cu stress concentrations. Additionally, it could also help in devising effective strategies for mitigating the impact of pollution on plants [ 45 , 46 , 47 , 49 , 50 , 51 ]. The results obtained from this study support the previous research on Cu toxicity conducted on plants such as hydrilla [ 52 ], seagrass [ 53 ] and moss Scopelophila cataractae [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Modeling DNA Methylation Profiles and Epigenetic Analysis of Safflower (Carthamus tinctorius L.) Seedlings Exposed to Copper Heavy Metal

Bolukbasi

Karakaş

2023

Toxics

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Heavy metals are chemical elements with high density that can be toxic or poisonous even at low concentrations. They are widely distributed in the environment due to industrial activities, mining, pesticide use, automotive emissions and domestic wastes. This study aimed to investigate the toxic effects of copper (Cu) heavy metal on safflower plants in terms of genetic and epigenetic parameters. Safflower seeds were exposed to different concentrations of Cu heavy metal solution (20, 40, 80, 160, 320, 640, 1280 mg L−1) for three weeks, and changes in the genomic template stability (GTS) and methylation pattern in the root tissues were analyzed using PCR and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques. The results indicated that high doses of Cu have genotoxic effects on the genome of safflower plants. Epigenetic analysis revealed four different methylation patterns, with the highest total methylation rate of 95.40% observed at a 20 mg L−1 concentration, and the lowest rate of 92.30% observed at 160 mg L−1. Additionally, the maximum percentage of non-methylation was detected at 80 mg L−1. These results suggest that changes in the methylation patterns can serve as an important mechanism of protection against Cu toxicity. Furthermore, safflower can be used as a biomarker to determine the pollution in soils contaminated with Cu heavy metal.

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“…DNA methylation, which adds the methyl group to the C-5 cytosine (5meC) position, is a common epigenetics marker in many eukaryotes and occurs predominantly in CpG dinucleotides (Liu et al 2021). Restriction enzymes with CpG detection sequences can distinguish between methylated and non-methylated states (Harshitha and Nair, 2020). CRED-RA is one of the DNA methylation detection techniques that has been studied by many researchers (Bakir andGüleray, 2021, Falahi et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Diversity of individuals' methylation patterns to different moisture regimes in Einkorn wheat revealed by CRED-RA technique

Hosseini,

Zarei,

Cheghamirza

2023

Genet Resour Crop Evol

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Among the wild relatives of wheat, diploid species with desirable characteristics have high potential in crop modi cation. One of the important epigenetic changes that is associated with the pattern of gene expression and causes genetic instability is DNA methylation. The coupled restriction enzyme digestionrandom ampli cation (CRED-RA) technique and ve RAPD primers were used to study methylation changes in two Einkorn wheat genotypes including S10 and H9 genotypes and four soil water availability treatments included control, mild, moderate and severe stress (irrigation at 100%, 75%, 50% and 25% of eld capacity, respectively) in two stages (14 days after water de ciency and 14 days after re-watering). The experiment was laid out as 2×4 factorial experiment within completely randomized design replicated three times. Measured physiological traits included relative leaf water content, Fv / Fm and stomatal conductance. After 14 days of exposure to drought stress, the results showed that the lowest relative water content of leaves, Fv/Fm and stomatal conductance were related to severe stress treatment. Also, S10 genotype was superior to H9 after application of moisture stress treatments and after re-watering. Different patterns of methylation were observed in different replications of each treatments in response to severe water de ciency, which can be due to the diversity of individuals' response to stress and the speci city of epigenetics changes. Most of the fragments that showed a change in methylation pattern due to water de ciency, after stress removal, returned to the state before stress, which indicates the reversibility of methylation changes in the genome.

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Evaluation of DNA Methylation Changes by CRED–RA Analysis Following Prednisone Treatment of Endophyte, Fusarium oxysporum

Cited by 6 publications

References 25 publications

Rutin hydrate induces autophagic cell death and oxidative stress response in phytopathogenic fungus Fusarium graminearum

Rutin hydrate induces autophagic cell death and oxidative stress response in phytopathogenic fungus Fusarium graminearum

Modeling DNA Methylation Profiles and Epigenetic Analysis of Safflower (Carthamus tinctorius L.) Seedlings Exposed to Copper Heavy Metal

Diversity of individuals' methylation patterns to different moisture regimes in Einkorn wheat revealed by CRED-RA technique

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