Evaluation of DNA Damage in Peripheral Blood Lymphocytes Exposed to Gamma Radiation

Rady, Aya; Dakrory, Amira; koraa, Sohier; Elshemey, Wael M.

doi:10.21608/jsrs.2020.96478

Cited by 2 publications

(2 citation statements)

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“…TM represents the center of the comet's head to the center of the comet's tail. At the same time, OTM shows the multiplication result of % T.DNA with TM [21].…”

Section: Discussionmentioning

confidence: 96%

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Adaptive Response of Peripheral Blood Lymphocytes in Medical Radiation Workers using the Comet Assay: A Preliminary Studyomet Assay: A Preliminary Study

Kisnanto

Darlina

Musthafa³

et al. 2022

Indonesian Journal of Cancer

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Background: Gamma irradiation can cause DNA damage in single and double-strand breaks (SSBs & DSBs), especially in peripheral blood lymphocytes. Radiotherapy medical radiation workers can be exposed to gamma radiation related to their daily work. The comet assay is a sensitive method for analyzing DNA damage, especially SSBs. This study explores DNA damage in medical radiation workers’ peripheral blood lymphocytes as an adaptive response using the comet assay.Methods: Blood samples were obtained from four radiotherapy medical radiation workers as a case study (MRW) and two non-medical radiation workers as controls, and then irradiated with various doses of 0, 1, 1.5, and 2 Gy. Lymphocytes were isolated by histopaque and processed by comet assay on the slide under alkaline conditions. The imaging results were analyzed using the Casplab_1.2.3b2 software. The comet assay parameters observed were Tail Length (TL), % Tail DNA (T.DNA), Tail Moment (TM), and Olive Tail Moment (OTM). The one-way ANOVA method was used to analyze statistically between treatment groups. Results: Based on the study results, an increase in TL, T.DNA, TM, and OTM values in all samples was directly proportional to the increase in radiation dose. However, there was no significant difference (P > 0.05) between the MRW group and the control group on each parameter of the comet assay.Conclusions: From this study, it can be concluded that the level of DNA damage of lymphocyte cells as part of the adaptive response in the MRW and control groups was relatively similar after exposure at doses of 0, 1, 1.5, and 2 Gy.

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“…TM represents the center of the comet's head to the center of the comet's tail. At the same time, OTM shows the multiplication result of % T.DNA with TM [21].…”

Section: Discussionmentioning

confidence: 96%

“…In addition, % T.DNA is directly proportional to DNA damage [4]. In their research, Rady et al [21] stated that TM and OTM also provide a positive…”

Section: Declarations Discussionmentioning

confidence: 98%

Adaptive Response of Peripheral Blood Lymphocytes in Medical Radiation Workers using the Comet Assay: A Preliminary Studyomet Assay: A Preliminary Study

Kisnanto

Darlina

Musthafa³

et al. 2022

Indonesian Journal of Cancer

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The potential effect of temulawak (Curcuma xanthorrhiza Roxb.) and garlic (Allium sativum L.) as a radioprotective agent against 6 Gy total body irradiation in rats

Kisnanto,

Tetriana,

Yusuf

et al. 2024

Radioprotection

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The main impact of gamma radiation on a biological system is the formation of Reactive Oxygen Species (ROS) and free radicals. The study aimed to explore the potential effect of temulawak (Curcuma xanthorrizha Roxb) and garlic (Allium sativum L) against 6 Gy total body irradiation (TBI) in rats by observing malondialdehide (MDA), glutathione (GSH) and comet assay. Twenty male rats were divided into five groups: control, 6 Gy, temulawak extract+6 Gy, garlic extract+6 Gy, and n-acetyl cysteine (NAC)+6 Gy. MDA and GSH were measured on liver and spleen tissue homogenates, while comet assay was on lymphocyte cells. Gamma irradiation at 6 Gy significantly increased the MDA level and comet assay compared to the control group, while the GSH level decreased (p < 0.05). Temulawak extract significantly reduced MDA levels and comet assay compared to the 6 Gy group, while GSH levels in the liver increased. Garlic extract significantly drops comet assay while increasing GSH levels in the liver. NAC decreases MDA levels in the liver and comet assay while increasing GSH levels in the spleen. It could be concluded that temulawak extract has a better radioprotective agent than garlic extract and is almost identical to NAC as a standard antioxidant.

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Evaluation of DNA Damage in Peripheral Blood Lymphocytes Exposed to Gamma Radiation

Cited by 2 publications

References 0 publications

Adaptive Response of Peripheral Blood Lymphocytes in Medical Radiation Workers using the Comet Assay: A Preliminary Studyomet Assay: A Preliminary Study

Adaptive Response of Peripheral Blood Lymphocytes in Medical Radiation Workers using the Comet Assay: A Preliminary Studyomet Assay: A Preliminary Study

The potential effect of temulawak (Curcuma xanthorrhiza Roxb.) and garlic (Allium sativum L.) as a radioprotective agent against 6 Gy total body irradiation in rats

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