Evaluation of diverse antioxidant activities of Galium aparine

Bokhari, Jasia; Khan, Muhammad Rashid; Shabbir, Maria; Rashid, Umer; Jan, Shumaila; Zai, Jawaid Ahmed

doi:10.1016/j.saa.2012.09.056

Cited by 39 publications

(31 citation statements)

References 25 publications

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“…Similar results were observed [24] in their study on Fagonia olivieri whole plant. However, not in agreement with the study of Bokhari et al [25], where they observed maximum DPPH scavenging activity in aqueous fraction of Gallium aparine .…”

Section: Discussioncontrasting

confidence: 83%

HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad

Shah

Khan

Sattar

et al. 2014

BMC Complement Altern Med

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BackgroundAsparagus gracilis subspecie of Asparagus capitatus Baker, is described as food and medicine for various ailments. In this study we investigated, its phenolic constituents, in vitro antioxidant potential against various free radicals and anti-urease potential.MethodsAsparagus gracilis aerial parts collected from District Islamabad, Pakistan were extracted with crude methanol which was further fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fraction. Total phenolic and flavonoid contents were estimated for extract and all the derived fractions. Diverse in vitro antioxidants assays such as DPPH, H2O2, •OH, ABTS, β-carotene bleaching assay, superoxide radical, lipid peroxidation, reducing power, and total antioxidant capacity were studied to assess scavenging potential. Antiurease activity of methanol extract and its derived fractions was also investigated. HPLC-DAD analysis of crude methanol extract was performed by using different phenolic standards.ResultsEthyl acetate fraction expressed maximum content of flavonoids (240.6 ± 6.1 mg RE/g dry sample), phenolics (615 ± 13 mg GAE/g dry sample) and best antioxidant potential among different fractions of crude methanol extract. Hydrogen peroxide assay and hydroxyl, supeoxide, nitric oxide free radicals antioxidant assays as well as beta carotene assay showed significant correlation with flavonoid content while hydrogen peroxide, ABTS and lipid peroxidation assay displayed significant correlation with phenolic content. HPLC analysis showed the presence of important phenolics i.e. catechin (4.04 ± 0.02 μg/mg sample), caffeic acid (0.89 ± 0.003 μg/mg sample), rutin (24.58 ± 0.1 μg/mg sample), myricetin (1.13 ± 0.07 μg/mg sample) and quercetin (14.91 ± 0.09 μg/mg sample). Ethyl acetate fraction expressed lowest IC50 in antiurease activity. Correlation analysis of antiurease activity expressed significant correlation with flavonoids (P < 0.004) and phenolics (P < 0.02) proposing multipotent activity of fractions.ConclusionThese results revealed the presence of some bioactive compound in the ethyl acetate fraction having both antioxidant as well as antiurease potential.

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Section: Discussioncontrasting

confidence: 83%

HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad

Shah

Khan

Sattar

et al. 2014

BMC Complement Altern Med

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“…Comparing the results obtained for the different extracts of G. aparine with those of trolox (positive control), the hydromethanolic extract of the freeze-dried sample was the only one revealing a lower EC 50 value (13.5 and 23 μg/ mL for the extract and the positive control, respectively), in the TBARS inhibition assay. In a study performed with several G. aparine extract fractions, the butanol, methanol, and aqueous fractions revealed DPPH scavenging activity at 61.7, 75, and 58.3 μg/mL, respectively, which represent concentrations ten times lower than those obtained in the present study (Bokhari et al, 2013). On the other hand, in a study performed by Abbasi et al (2015), water and acetone extracts of G. aparine showed approximately 69 and 55% DPPH scavenging activity, respectively, in concentrations of 100 mg/mL dw, but once again, it was not possible to compare these results to those achieved herein because of the methodology discrepancies along with the differences on the results analysis and presentation.…”

Section: Antioxidant Activity and Cytotoxicitycontrasting

confidence: 84%

“…These authors also studied three other Galium species, namely Galium verum, Galium mollugo, and Galium odoratum and despite G. aparine presented the largest variety of phenolic compounds, G. verum was the species revealing the highest total phenolic concentration, followed by G. mollugo, G. aparine, and G. odoratum (894, 31, 21, and 17 mg/100 g dw, respectively). Other studies also evaluated G. aparine water extracts (Abbasi et al, 2015) and aqueous fractions (Bokhari et al, 2013) in terms of total phenolics and the water extracts contained 24 mg GAE/100 g fw, whereas the aqueous fractions revealed a slightly lower amount of 22.1 mg GAE/g of dried fraction.…”

Section: Phenolic Compoundsmentioning

confidence: 99%

Dehydration process influences the phenolic profile, antioxidant and antimicrobial properties of Galium aparine L.

Senio

Pereira

Vaz

et al. 2018

Industrial Crops and Products

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A B S T R A C TGalium aparine L. is a very disseminated plant in temperate zones, commonly known as clivers or bedstraw, belonging to the Rubiaceae family and it is traditionally used for its medicinal applications. In this study, G. aparine hydromethanolic extracts and infusions were prepared from air-dried and freeze-dried samples in order to assess their phenolic profile, antioxidant, antimicrobial, and cytotoxic properties. All the studied extracts revealed a similar phenolic profile, but the hydromethanolic extract obtained from the freeze-dried sample presented the highest concentration of phenolic compounds, followed by the respective infusion and the airdried sample hydromethanolic extract. The major compound detected in the extracts was 5-O-caffeoylquinic acid (from 145 to 163 mg/g extract). Regarding the bioactivity, in general, the extracts presenting higher phenolic concentrations also revealed enhanced bioactive properties. The EC 50 values obtained in the antioxidant activity assays ranged from 13.5 to 884 μg/mL, with the freeze-dried sample hydromethanolic extract presenting the highest activity (13.5-555 μg/mL). Similar conclusions could be made in terms of antimicrobial properties, with this extract showing the lowest MIC (1.85-15 mg/mL), MBC (3.75-7.5 mg/mL), and MFC (3.75-20 mg/mL) values. None of the extracts revealed cytotoxicity. The results obtained in this study suggested that G. aparine extracts can be a good source of phenolic compounds with antioxidant and antimicrobial properties.

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“…The reducing power capacity is a useful method for measuring antioxidant activities [51]. In this assay, hydroxyl radicals are generated by the reaction of Fe 2+ and H 2 O 2 , and the antioxidant agents reduce the generation of hydroxyl radicals by chelating Fe 2+ [52]. The results of this activity (Table 3) showed that the EOs of the two plants have a comparable reducing capacity against the ferric ion; 14.73±0.25 mg/ml for T. blecherianus and 11.86±0.15 mg/ml for T. riatarum which is lowest than T. serpyllum and T. algeriensis EO (IC 50 = 0.66 µg/ml and 0.68 µg/ml, respectively) [49] and lowest than wild and cultivated T. maroccanus with IC 50 = 139.31±1.08 and 149.41±1.13 µg/ml, respectively [17].…”

Section: Ferric Reducing/antioxidant Power (Frap) Capacitymentioning

confidence: 99%

Chemical Composition, Antimicrobial, and Antioxidant Activities of Two Moroccan Thymus Essential Oils

Aouam

Atki

Taroq

et al. 2019

Asian J Pharm Clin Res

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Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.

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Evaluation of diverse antioxidant activities of Galium aparine

Cited by 39 publications

References 25 publications

HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad

HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad

Dehydration process influences the phenolic profile, antioxidant and antimicrobial properties of Galium aparine L.

Chemical Composition, Antimicrobial, and Antioxidant Activities of Two Moroccan Thymus Essential Oils

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