This experiment aimed to examine the potential of distillers dried grains soluble (DDGS) meal as a substitute for soybean meal (SBM) in the Paci c shrimp diet. The study aimed to investigate how DDGS affected disease resistance, antoxidation, and gut microbiota. 840 shrimp were given varied diets with varying concentrations of DDGS. D1 (0%), D2 2%, D3 4%, D4 6%, D5 8%, D6 10%, and D7 12% were the DDGS levels. Each group had three replicates of the 56-day experiment. Initially, the weight was 0.19 ± 0.00 g. The survival rate was consistently 100% for shrimp-fed DDGS, except for group 7 (14%) and the control group, where shrimp mortality was observed. The total protein (TP) content was improved as the inclusion level of DDGS increased. The glucose (GLU) exhibited a downward trend with higher DDGS inclusion.Similarly, the TG and CHO levels in the serum decreased as the DDGS levels increased. The activities of ALT (alanine transaminase) and AST (aspartate aminotransferase) decreased as the dietary DDGS increased, with the control group showing signi cantly higher ALT and AST activities than those fed DDGS diets (P < 0.05). Furthermore, the SOD (superoxide dismutase) activity signi cantly increased with higher DDGS levels, while the MDA (malondialdehyde) activity showed the opposite trend. Notably, the control exhibited the highest MDA activity value. The most prevalent phylu found in the gut microbiota were Proteobacteria, and Bacteroidetes. Upon completion of a 7-day bacteria challenge, it was observed that shrimp fed with the control diet had a lower mortality rate compared to those fed the DDGS diet. Based on the parameters measured in this study, it was determined that replacing 10% of SBM with DDGS in the feed of Paci c shrimp is feasible.
The calculation for shrimp survivalSurvival rate % = [100× End number of shrimp/ Initial number of shrimp]
Serum biochemical analysisThis study set out to look into the antioxidant activity and serum biochemical parameters of Paci c shrimp. The levels of TG, CHO, GLU, TP, ALT, AST, MDA, and SOD were among the many activities that our team examined. We used assay kits from the prestigious Nanjing Jiancheng Bioengineering Institute in China to guarantee precise and trustworthy results. We used commercial assay kits from the Nanjing Jiancheng Institute in Nanjing, China, to measure ALT and AST levels. The biuret method was used to determine TP levels. SOD activity was measured using the nitro blue tetrazolium reaction, with the color extracted into butanol and measured at 550 nm. The TBA (thiobarbituric acid) test was used to measure the amount of MDA in the hepatopancreas. Lipid hydroperoxide breakdown was also measured using TBA, resulting in red chemicals at the 532 nm absorbance peak.
The analysis of digestive enzymeTo conduct a thorough analysis, we studied how dietary DDGS affects the activity of digestive enzymes, speci cally amylase, lysine, and protease, in shrimp. We selected three shrimp for each group and carefully removed and dissected their intestines. The intestine...
